全文获取类型
收费全文 | 1604篇 |
免费 | 103篇 |
国内免费 | 13篇 |
专业分类
化学 | 1191篇 |
晶体学 | 11篇 |
力学 | 35篇 |
数学 | 161篇 |
物理学 | 322篇 |
出版年
2023年 | 4篇 |
2022年 | 12篇 |
2021年 | 44篇 |
2020年 | 22篇 |
2019年 | 36篇 |
2018年 | 29篇 |
2017年 | 23篇 |
2016年 | 57篇 |
2015年 | 43篇 |
2014年 | 66篇 |
2013年 | 98篇 |
2012年 | 138篇 |
2011年 | 151篇 |
2010年 | 106篇 |
2009年 | 82篇 |
2008年 | 111篇 |
2007年 | 98篇 |
2006年 | 82篇 |
2005年 | 86篇 |
2004年 | 62篇 |
2003年 | 60篇 |
2002年 | 63篇 |
2001年 | 30篇 |
2000年 | 24篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 14篇 |
1995年 | 15篇 |
1994年 | 16篇 |
1993年 | 11篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 11篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1934年 | 1篇 |
排序方式: 共有1720条查询结果,搜索用时 15 毫秒
51.
p21Cip/WAF1, an important regulator of cell proliferation, is induced by both p53- and extracellular signal regulated kinase (ERK) pathways. The induction of p21Cip/WAF1 occurs by prolonged activation of the ERKs caused by extracellular stimuli, such as zinc. However, not all the cells appeared to respond to ERK pathway dependent p21Cip/WAF1 induction. Here we investigated the cause of such difference using colorectal cancer cells. p21Cip/WAF1 induction and concomitant reduction of bromodeoxyuridine (BrdU) incorporation were observed by zinc treatment within HT-29 and DLD-1. However, HCT-116 cells with high endogenous p21Cip/WAF1 levels did not show any additional increment of p21Cip/WAF1 levels by zinc treatment and did maintain high BrdU incorporation level. The p21Cip/WAF1 induction by zinc depended upon prolonged activation of extracellular signal regulated kinase (ERK) was not observed in HCT-116 cells. The percentage of BrdU positive cells was 50% higher in p21Cip/WAF1 -/- HCT-116 cells compared to p21Cip/WAF1 +/+ HCT- 116 cells, and no cells induced p21Cip/WAF1 incorporated BrdU in its nucleus, yet confirming the importance of p21Cip/WAF1 induction in anti- proliferation. These results again support that p21Cip/WAF1 induction is a determinant in the regulation of colonic proliferation by the ERK pathway. 相似文献
52.
Jung HW La SJ Kim JY Heo SK Kim JY Wang S Kim KK Lee KM Cho HR Lee HW Kwon B Kim BS Kwon BS 《Experimental & molecular medicine》2003,35(6):501-508
Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+) and CD8(+) T cells, CD19(+) B cells, CD14(+) monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders. 相似文献
53.
54.
[reaction: see text] Highly stereodivergent Woodward-Prevost reaction applied to iodoacetates derived from homochiral alpha-amino acids afforded enantiopure 3,4-cis- and 3,4-trans-pyrrolidinediol derivatives, with control over the protecting group, allowing for differential protection. 相似文献
55.
Aryl pyridyl ethers were prepared from phenols and active halopyridines under essentially neutral condition via fluoride ion assisted reaction. 相似文献
56.
57.
Kyeong‐Ryoon Lee Sung Heum Choi Jin‐Sook Song Hyewon Seo Yoon‐Jee Chae Hwang Eui Cho Jin Hee Ahn Sung‐Hoon Ahn Myung Ae Bae 《Biomedical chromatography : BMC》2013,27(7):846-852
In this study, we developed a method for the determination of PF‐04620110 (2‐{(1r,4r)‐4‐[4‐(4‐amino‐5‐oxo‐7,8‐dihydropyrimido[5,4‐f][1,4]oxazepin‐6(5H)‐yl)phenyl]cyclohexyl}acetic acid), a novel diacylglycerol acyltransferase 1 (DGAT‐1) inhibitor, in rat plasma and validated it using liquid chromatography–tandem mass spectrometry (LC‐MS/MS). Rat plasma samples were processed following a protein precipitation method by using acetonitrile and were then injected into an LC‐MS/MS system for quantification. PF‐04620110 and imipramine (internal standard) were separated using a Hypersil Gold C18 column, with a mixture of acetonitrile and 10 mm ammonium formate (90:10, v/v) as the mobile phase. The ion transitions monitored in positive‐ion mode [M + H]+ of multiple‐reaction monitoring were m/z 397.0 → 260.2 for PF‐04620110 and m/z 280.8 → 86.0 for imipramine. The detector response was specific and linear for PF‐04620110 at concentrations within the range 0.05–50 µg/mL and the signal‐to‐noise ratios for the samples were ≥10. The intra‐ and inter‐day precision and accuracy of the method matched the acceptance criteria for assay validation. PF‐04620110 was stable under various processing and/or handling conditions. PF‐04620110 concentrations in the rat plasma samples could be measured up to 24 h after intravenous or oral administration of PF‐04620110, suggesting that the assay is useful for pharmacokinetic studies in rats. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
58.
59.