Staining of Human Thyroarytenoid Muscle with Myosin Antibodies Reveals Some Unique Extrafusal Fibers, but no Muscle Spindles

This study describes the myosin composition of extrafusal and intrafusal muscle fibers found in the human thyroarytenoid (TA) and sternohyoid (control) muscles. We sought to determine the presence of muscle spindles in the TA muscle, and to identify unusual extrafusal fiber types, using the commonly accepted approach of tissue staining with myosin isoform specific antibodies. Extrafusal fibers are organized into motor units, which subsequently produce muscle movement, whereas intrafusal fibers compose muscle spindles, the primary stretch receptor that provides afferent (feed back) information to the nervous system for regulation of motor unit length and tonicity. Immunohistochemical identification of muscle spindles was confirmed in sternohyoid, but not in TA samples; however, some extrafusal fibers contained tonic myosin. These results indicate that human TA muscle functions similar to some mammalian extraocular muscle, performing unloaded (non-weight bearing) contractions without afferent information from native muscle spindles.     

Constructive existence theorems for problems of Thomas-Fermi Type

A minimal positive solution of the Thomas-Fermi problem ? = λt^?1/2 w^3/2, w(0) = 1, w(1) = w(1) is shown to exist for each λ > 0. It is proved that all positive solutions, for a given value of λ, are strictly ordered and that the minimal positive solution w_λ is a decreasing function of λ. Upper and lower analytic bounds for w _λ are given and these bounds are shown to initiate sequences of Picard and Newton iterates which converge monotonically to w _λ. A comparative analysis of the efficiency of the iteration schemes is presented. The methods used are of a general nature and can be applied to a variety of nonlinear boundary value problems of convex type [14].     

An ecological acoustic recorder (EAR) for long-term monitoring of biological and anthropogenic sounds on coral reefs and other marine habitats

Keeping track of long-term biological trends in many marine habitats is a challenging task that is exacerbated when the habitats in question are in remote locations. Monitoring the ambient sound field may be a useful way of assessing biological activity because many behavioral processes are accompanied by sound production. This article reports the preliminary results of an effort to develop and use an Ecological Acoustic Recorder (EAR) to monitor biological activity on coral reefs and in surrounding waters for periods of 1 year or longer. The EAR is a microprocessor-based autonomous recorder that periodically samples the ambient sound field and also automatically detects sounds that meet specific criteria. The system was used to record the sound field of coral reefs and other marine habitats on Oahu, HI. Snapping shrimp produced the dominant acoustic energy on the reefs examined and exhibited clear diel acoustic trends. Other biological sounds recorded included those produced by fish and cetaceans, which also exhibited distinct temporal variability. Motor vessel activity could also be monitored effectively with the EAR. The results indicate that acoustic monitoring may be an effective means of tracking biological and anthropogenic activity at locations where continuous monitoring by traditional survey methods is impractical.     

The Lattice of Ordered Compactifications of a Direct Sum of Totally Ordered Spaces

The lattice of ordered compactifications of a topological sum of a finite number of totally ordered spaces is investigated. This investigation proceeds by decomposing the lattice into equivalence classes determined by the identification of essential pairs of singularities. This lattice of equivalence classes is isomorphic to a power set lattice. Each of these equivalence classes is further decomposed into equivalence classes determined by admissible partially ordered partitions of the ordered Stone–ech remainder. The lattice structure within each equivalence class is determined using an algorithm based on the incidence matrix of the partially ordered partition. As examples, the ordered compactification lattices for the spaces [0,1)[0,1),[0,1)[0,1)[0,1),RR, and R/{0}R/{0} are determined.     

Tabu search for a class of scheduling problems

Scheduling problems are often modeled as resourceconstrained problems in which critical resource assignments to tasks are known and the best assignment of resource time must be made subject to these constraints. Generalization toresource scheduling, where resource assignments are chosen concurrently with times results is a problem which is much more difficult. A simplified model of the general resource scheduling model is possible, however, in which tasks must be assigned a singleprimary resource, subject to constraints resulting from preassignment ofsecondary, or auxiliary, resources. This paper describes extensions and enhancements of tabu search for the special case of the resource scheduling problem described above. The class of problems is further restricted to those where it is reasonable to enumerate both feasible time and primary resource assignments. Potential applications include shift oriented production and manpower scheduling problems as well as course scheduling where classrooms (instructors) are primary and instructors (rooms) and students are secondary resources. The underlying model is a type of quadratic multiple choice problem which we call multiple choice quadratic vertex packing (MCQVP). Results for strategic oscillation and biased candidate sampling strategies are shown for reasonably sized real and randomly generated, synthetic, problem instances. The strategies are compared with other variations using consistent measures of solution time and quality developed for this study.     

Cross-validation and evaluation of the performance of methods for the elemental analysis of forensic glass by μ-XRF, ICP-MS, and LA-ICP-MS

Elemental analysis of glass was conducted by 16 forensic science laboratories, providing a direct comparison between three analytical methods [micro-x-ray fluorescence spectroscopy (μ-XRF), solution analysis using inductively coupled plasma mass spectrometry (ICP-MS), and laser ablation inductively coupled plasma mass spectrometry]. Interlaboratory studies using glass standard reference materials and other glass samples were designed to (a) evaluate the analytical performance between different laboratories using the same method, (b) evaluate the analytical performance of the different methods, (c) evaluate the capabilities of the methods to correctly associate glass that originated from the same source and to correctly discriminate glass samples that do not share the same source, and (d) standardize the methods of analysis and interpretation of results. Reference materials NIST 612, NIST 1831, FGS 1, and FGS 2 were employed to cross-validate these sensitive techniques and to optimize and standardize the analytical protocols. The resulting figures of merit for the ICP-MS methods include repeatability better than 5 % RSD, reproducibility between laboratories better than 10 % RSD, bias better than 10 %, and limits of detection between 0.03 and 9 μg g^?1 for the majority of the elements monitored. The figures of merit for the μ-XRF methods include repeatability better than 11 % RSD, reproducibility between laboratories after normalization of the data better than 16 % RSD, and limits of detection between 5.8 and 7,400 μg g^?1. The results from this study also compare the analytical performance of different forensic science laboratories conducting elemental analysis of glass evidence fragments using the three analytical methods.