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排序方式: 共有154条查询结果,搜索用时 281 毫秒
31.
32.
Boroduleva Anna Yu. Manclús Juan J. Montoya Ángel Eremin Sergei A. 《Analytical and bioanalytical chemistry》2018,410(26):6923-6934
Analytical and Bioanalytical Chemistry - Fluorescence polarization immunoassays (FPIAs) for thiabendazole and tetraconazole were first developed. Tracers for FPIAs of thiabendazole and... 相似文献
33.
A fully automated method for leaching of TCP (3,5,6-trichloro-2-pyridinol, the major degradation product of the widely used chlorpyrifos insecticides) from soil and fruit with subsequent determination by ELISA is reported. The automation of the weighing and leaching steps enables unattended development of sample preparation. The determination of the target analyte, traditionally performed by liquid chromatography, has been substituted by specific immunoassay using a non-commercial monoclonal antibody which enables the determination of TCP within the range 0.01-7 ng 1(-1) with an R.S.D. of 8.6%. 相似文献
34.
Mauriz E Calle A Manclús JJ Montoya A Lechuga LM 《Analytical and bioanalytical chemistry》2007,387(4):1449-1458
Multi-analyte detection of environmentally relevant pesticides is performed by using a two-channelled surface plasmon resonance
(SPR) biosensor. The special design of the SPR instrument allows the determination of several analytes (DDT, chlorpyrifos
and carbaryl) via different immobilization formats. First, simultaneous pesticide monitoring is possible by flowing chlorpyrifos,
carbaryl or DDT samples separately over each channel of the SPR system, wherein their corresponding recognition element was
previously immobilized. The second approach is based on the multiple and combined immobilization of several analyte recognition
elements on the sensing surface of one individual flow cell. In this format, the analysis time for all three pesticides varied
from 40 to 60 min depending on the number of regeneration cycles. In most cases, similar detection limits were attained for
the target analyte irrespective of the assay format, with sensitivity values at the nanogram per litre level (18–50 ng L−1). The assay reproducibility was proved through the repeated use of the same sensor surface for over more than 200 assay cycles,
whereas the absence of biosensor response to non-related analytes showed the specificity and reliability of the analysis.
The SPR instrument, including optics, electronics and microfluidics, is already commercialised by the company SENSIA, SL. 相似文献
35.
A piezoelectric immunosensor for the determination of pesticide residues and metabolites in fruit juices 总被引:4,自引:0,他引:4
A quartz crystal microbalance (QCM) immunosensor was developed for the determination of the insecticide carbaryl and 3,5,6-trichloro-2-pyridinol (TCP), the main metabolite of the insecticide chlorpyrifos and of the herbicide triclopyr. The detection was based on a competitive conjugate-immobilized immunoassay format using monoclonal antibodies (MAbs). Hapten conjugates were covalently immobilized, via thioctic acid self-assembled monolayer (SAM), onto the gold electrode sensitive surface of the quartz crystal. This covalent immobilization allowed the reusability of the modified electrode surface for at least one hundred and fifty assays without significant loss of sensitivity. The piezoimmunosensor showed detection limits (analyte concentrations producing 10% inhibition of the maximum signal) of 11 and 7 μg l−1 for carbaryl and TCP, respectively. The sensitivity attained (I50 value) was around 30 μg l−1 for both compounds. Linear working ranges were 15-53 μg l−1 for carbaryl and 13-83 μg l−1 for TCP. Each complete assay cycle took 20 min. The good sensitivity, specificity, and reusability achieved, together with the short response time, allowed the application of this immunosensor to the determination of carbaryl and TCP in fruits and vegetables at European regulatory levels, with high precision and accuracy. 相似文献
36.
Vergel C Montoya R Mendiguchía C García-Vargas M Moreno C 《Analytical and bioanalytical chemistry》2012,404(3):665-670
In the last years, some analytical methodologies have been identified as a source of pollution, receiving increasing attention to decrease their impact on the environment. In this sense, the so-called solvent-less methodologies appear as a green alternative to reduce the volume of solvents used in many sample treatment procedures and, consequently, the volume of toxic wastes produced. Among these techniques, analytical methodologies based on liquid-phase microextraction are being continuously developed, although most applications are focused on organic compounds. In this work, a three-phase hollow-fibre liquid-phase microextraction (HF-LPME) system has been developed for the preconcentration of nickel in natural waters, prior to the analysis by atomic absorption spectrometry. Under optimum conditions, the new system allowed an enrichment factor of 29.80 to be obtained after 60 min of experiment, and it was successfully applied to the determination of nickel in both saline and non-saline water samples, at ppb and ppt levels. The results were compared with those obtained using a well-established methodology based on liquid solvent extraction showing no significant differences (α = 0.05) between both values. In addition, the new HF-LPME presents the advantages of a green analytical technique, as its greenness profile shows, with the additional reduction of sample manipulation and time cost. 相似文献
37.
Antonio Doménech Noemí Montoya Javier Alarcón 《Journal of Solid State Electrochemistry》2012,16(3):963-975
The voltammetry of nanoparticles and scanning electrochemical microscopy are applied to characterize praseodymium centers
in tetragonal and monoclinic zirconias, doped with praseodymium ions (Pr
x
Zr1−x
O2), prepared via sol–gel routes. Doped zirconia nanoparticles were synthesized by a sol–gel liquid-phase route and characterized
by different techniques, including X-ray diffraction powder pattern, ultraviolet–visible diffuse reflectance spectroscopy,
infrared spectroscopy, and transmission electron microscopy (TEM). Gels annealed at around 400 °C yielded tetragonal Pr
x
Zr1−x
O2 phases. The monoclinic forms of Pr-doped ZrO2 were obtained by annealing at temperatures higher than 1,100 °C. TEM micrographs proved that the size of the nanoparticles
produced was dependent on their crystalline form, around 15 and 60 nm for tetragonal and monoclinic, respectively. The electrochemical
study confirmed that a relatively high content of praseodymium cation was in the chemical state (IV), i.e., as Pr4+, in both zirconia host lattices. The catalytic and photocatalytic effects of Pr4+ centers located in the monoclinic zirconia lattice on nitrite reduction and oxygen evolution reaction were studied. 相似文献
38.
We study some properties of the quotient forcing notions ${Q_{tr(I)} = \wp(2^{< \omega})/tr(I)}$ and P I ?= B(2 ω )/I in two special cases: when I is the σ-ideal of meager sets or the σ-ideal of null sets on 2 ω . We show that the remainder forcing R I =?Q tr(I)/P I is σ-closed in these cases. We also study the cardinal invariant of the continuum ${\mathfrak{h}_{\mathbb{Q}}}$ , the distributivity number of the quotient ${Dense(\mathbb{Q})/nwd}$ , in order to show that ${\wp(\mathbb{Q})/nwd}$ collapses ${\mathfrak{c}}$ to ${\mathfrak{h}_{\mathbb{Q}}}$ , thus answering a question addressed in Balcar et?al. (Fundamenta Mathematicae 183:59–80, 2004). 相似文献
39.
Mara Mirasoli Angela Buragina Luisa Stella Dolci Massimo Guardigli Patrizia Simoni Angel Montoya Elisabetta Maiolini Stefano Girotti Aldo Roda 《Analytica chimica acta》2012
Simple, rapid and highly sensitive assays, possibly allowing on-site analysis, are required in the security and forensic fields or to obtain early signs of environmental pollution. Several bioanalytical methods and biosensors based on portable devices have been developed for this purpose. Among them, Lateral Flow ImmunoAssays (LFIAs) offer the advantages of rapidity and ease of use and, thanks to the high specificity of antigen–antibody binding, allow greatly simplifying and reducing sample pre-analytical treatments. However, LFIAs usually employ colloidal gold or latex beads as labels and they rely on the formation of colored bands visible by the naked eye. With this assay format, only qualitative or semi-quantitative information can be obtained and low sensitivity is achieved. Recently, the use of enzyme-catalyzed chemiluminescence detection in LFIA has been proposed to overcome these problems. In this work, we describe the development of a quantitative CL-LFIA assay for the detection of 2,4,6-trinitrotoluene (TNT) in real samples. Thanks to the use of a portable imaging device for CL signal measurement based on a thermoelectrically cooled CCD camera, the analysis could be performed directly on-field. A limit of detection of 0.2 μg mL−1 TNT was obtained, which is five times lower than that obtained with a previously described colloidal gold-based LFIA developed employing the same immunoreagents. The dynamic range of the assay extended up to 5 μg mL−1 TNT and recoveries ranging from 97% to 111% were obtained in the analysis of real samples (post blast residues obtained from controlled explosion). 相似文献
40.
Eduardo H. Montoya Pablo A. Mendoza Patricia S. Bedregal Oscar R. Baltuano Isaac M. Cohen 《Journal of Radioanalytical and Nuclear Chemistry》2012,291(1):175-178
A method that combines the use of non-destructive neutron activation analysis and high-resolution α spectrometry has been
developed for determination of the activities of 234U and 238U in geological samples of low uranium content. The 238U content is determined by k0-based neutron activation analysis, whereas the 234U/238U relationship is measured by α spectrometry after isolation and electrodeposition of the uranium extracted from a lixiviation
with 6 M HCl. The main advantage of the method is the simplicity of the chemical operations, including the fact that the steps
destined to assure similar chemical state for the tracer and the uranium species present in the sample are not necessary.
The method was applied to soil samples from sites of the North Peru Coast. Uranium concentration range 3–40 mg/kg and the
isotopic composition correspond to natural uranium, with about 10% uncertainty. 相似文献