Abelmoschus esculentus (Okra) is an important vegetable crop, widely cultivated around the world due to its high nutritional significance along with several health benefits. Different parts of okra including its mucilage have been currently studied for its role in various therapeutic applications. Therefore, we aimed to develop and characterize the okra mucilage biopolymer (OMB) for its physicochemical properties as well as to evaluate its in vitro antidiabetic activity. The characterization of OMB using Fourier-transform infrared spectroscopy (FT-IR) revealed that okra mucilage containing polysaccharides lies in the bandwidth of 3279 and 1030 cm−1, which constitutes the fingerprint region of the spectrum. In addition, physicochemical parameters such as percentage yield, percentage solubility, and swelling index were found to be 2.66%, 96.9%, and 5, respectively. A mineral analysis of newly developed biopolymers showed a substantial amount of calcium (412 mg/100 g), potassium (418 mg/100 g), phosphorus (60 mg/100 g), iron (47 mg/100 g), zinc (16 mg/100 g), and sodium (9 mg/100 g). The significant antidiabetic potential of OMB was demonstrated using α-amylase and α-glucosidase enzyme inhibitory assay. Further investigations are required to explore the newly developed biopolymer for its toxicity, efficacy, and its possible utilization in food, nutraceutical, as well as pharmaceutical industries. 相似文献
Flurbiprofen loaded PCL/PVP blend microspheres were prepared by o/w solvent evaporation method using various concentrations
of gelatin as emulsifying agent. Microsphere recovery decreased with a decrease in the concentration of the emulsifier in
the dispersion. Encapsulation efficiency and drug loading of microspheres increased with decrease in concentration of emulsifying
agent. Hydration rate, encapsulation efficiency and drug loading of microspheres increased with increase in concentration
of PVP. Rheological properties showed free flowing nature of microspheres. SEM (Scanning electron microscope) revealed microspheres
were discrete, spherical and became porous with decrease in concentration of emulsifying agent but smooth with higher concentration
of emulsifying agent. FTIR (Fourier transform infrared spectroscopy) spectra of pure and encapsulated flurbiprofen in all
formulation showed no significant difference in characteristic peaks, suggesting stability of flurbiprofen during encapsulation
process. X-RD (X-ray powder diffractometry) of pure flurbiprofen shows sharp peaks, which decreases on encapsulation, indicating
dispersion at molecular level and hence decrease in the crystallinity of drug in microspheres. Microspheres showed an enteric
nature at pH 1.2 and a sustained release pattern at pH 6.8. Rapid drug release was observed in microspheres with higher concentration
of PVP (polyvinylpyrrolidone), PVP acts as channeling agent. Formulation with low concentration of emulsifying agent also
showed a fast release due to porous structure. Drug release kinetics followed zero order at pH 1.2 while at pH 6.8 Higuchi
model was best fitted and was found non fickian. 相似文献
The gas-phase thermal decomposition of cis-2-methylcyclopropane carboxylic acid was investigated in the temperature range
692–753 K and pressure between 10 and 70 Torr. Arrhenius parameters were determined for homogeneous, unimolecular formation
of the isomeric products and for the overall loss-rate of the reactant. The determined values are in accordance with the Arrhenius
parameters that were reported previously for the thermal unimolecular reactions of cyclopropane and other substituted cyclopropanes.
The formation of isomeric products and the observed Arrhenius parameters are consistent with a biradical mechanism. The effect
of surface on the reaction was studied at 732 K using the packed reaction vessel. It was observed that the rate of production
of all isomeric products and the total loss of cis-2-methylcyclopropane carboxylic acid were not affected by increasing surface
to volume ratio. 相似文献
We extend to multilinear Hankel operators the fact that some truncations of bounded Hankel operators are still bounded. We prove and use a continuity property of bilinear Hilbert transforms on products of Lipschitz spaces and Hardy spaces.
This papers describes the in vitro interaction of copper(II) acetylsalicylate and copper(II) salicylate with genomic DNA isolated from human blood. The two drug substances were found to bind to DNA after incubation with whole blood over night. Bonding was confirmed by detection of separated DNA electrophoresis bands for copper, copper(II) acetylsalicylate, copper(II) salicylate, acetylsalicylic acid and salicylic acid. Drug–DNA interactions were observed during electrophoresis in the form of fragmentation by formation of two bands when compared to controls. Gel permeation chromatography parameters also confirmed the occurrence of fragmentation. The use of gel permeation chromatography parameters as a measure of fragmentation of DNA is discussed. The fragmentation of genomic DNA after incubation with copper(II) acetylsalicylate and copper(II) salicylate suggested that these drug substances might be responsible for cytotoxicity in vivo.