Histochemical and ultrastructural changes of sternomastoid muscle in aged wistar rats

The aim of this study was to evaluate histochemically and ultrastructurally the sternomastoid muscle (SM) of adults and aged rats, employing histochemic (NADH-TR reaction) and transmission electron microscopic methods. It was used 20 rats, divided into two groups: adults (n = 10), animals with 4 months of age, and aged group (n = 10), animals with 24 months of age. Five animals from each group were anesthetized with an overdose of urethane (3 g/kg i.p.), and the muscles dissected after the samples processing for histochemical reaction (NADH-TR). Three types of fibers were identified by their metabolic characteristics: fibers with high oxidative capacity (O), intermediate oxidative capacity (OG) and low oxidative capacity (G). For transmission electron microscopic method, the animals were anesthetized and perfused by modified Karnovsky solution and the tissues were postfixed in 1% osmium tetroxide solution, dehydrated and embedded in Spurr resin. It was performed ultra-thin sections for transmission electron microscopic analysis. The SM showed heterogeneity in their composition according to the fiber types, with significant difference (p < 0.05) when comparing the fibers types between the superficial and deep regions and between the adult and aged groups. It was observe a decrease between the comparison of the total fibers density and GO fiber, and an increase of the O fiber in aged group. Ultrastructural characteristics of muscle cells in aged group showed typical morphological changes, characterizing muscular atrophy. We conclude based on physiological ageing process, changes in muscle fibers classification, and ultrastructuraly, morphological alterations on muscle cells, characterizing a muscular atrophy.     

Effects of low-level laser irradiation in ultrastructural morphology, and immunoexpression of VEGF and VEGFR-2 of rat masseter muscle

The present study evaluates by ultrastructural and immunohistochemical methods, the possible changes on muscular tissue affected by LLLI during a treatment, for example, in cases of temporomandibular joint disorders. Sixty male Wistar rats divided into 6 groups (n=10) received ten laser irradiations, with different energy densities (groups I-0; II-0.5; III-1.0; IV-2.5; V-5.0; and VI-20 J/cm(2)). Muscles were removed and processed for transmission electron microscopic and immunohistochemical (VEGF and VEGFR-2) analyses. Captured photomicrographs of immunohistochemistry and transmission electron microscopy were evaluated. It was observed in the irradiated muscles, mitochondria of different shapes and sizes, with increased plasticity evidenced by organelles in fusion, division and the presence of elongated structures with characteristics of mitochondria, proximity with the dilated sarcoplasmatic reticulum, suggesting organelles with large amounts of energy, and the presence of cytoplasmic protrusions in the capillaries with high dosages. All studied groups showed immunostainings for both markers (VEGF and VEGFR-2), but in general those who received higher doses also showed the markings more pronounced, suggesting dose-dependent biomodulation. It was concluded that the LLLI was able to modify the ultrastructural characteristics and immunohistochemical pattern of VEGF and VEGFR-2 in the masseter muscle of rats.