Radiation Chemical Studies of Protein Reactions: Effect of Post-irradiation on the Breaking of Secondary Bonding in Protein

When protein was irradiated by prays from a ⁶⁰Co source, a post-irradiation effect of the breaking of secondary bonding in protein was caused. An empirical equation for the reactivity decay was obtained, and the phenomena were explained on the basis of molecular mechanism.     

FTIR-ATR evaluation of organic contaminant cleaning methods for SiO2 surfaces.

The effectiveness of cleaning organic contaminants from silicon dioxide (SiO2) surfaces was studied by conducting highly sensitive measurements using Fourier Transform Infrared Attenuated Total reflectance (FTIR-ATR) with a Si prism as the waveguide. To serve as an example, the surface of the prism was oxidized to an order of a few nanometers. The oxidized Si surface film was allowed to stand in the atmosphere and then wet-cleaned in a repeated manner; subsequently its thickness was measured by ellipsometry. Although, various wet-cleaning methods were tested, they only showed values of 0.1-0.2 nm larger than, but not equal to, the original thickness immediately after oxidation. FTIR-ATR measurements of the spectral change after exposure to air revealed that organic species, such as C-CH3 and -(CH2)n-, increased with time. Wet-cleaning the sample failed to remove the C-CH3 species, which indicates that they corresponded to the film thickness increment from the original.     

Capacitary Upper Estimates for Symmetric Dirichlet Forms

Upper estimates for capacities are studied for symmetric Dirichlet forms which do not necessarily admit intrinsic metric. Firstly, a general method of obtaining a sharp estimate from upper estimates for cut-off functions are provided in the local and non-local cases. Secondly, a capacitary upper estimate is established for the skew product of two symmetric Dirichlet forms for which suitable capacitary estimates are given. Several examples of capacitary upper inequalities in the local and non-local cases are also given. Especially, an upper estimate for the capacity is proved for the symmetric Dirichlet form associated to the Markov process subordinated (in the Bochner sense) to the skew product of two one-dimensional Brownian motions with respect to the local time of the first Brownian motion at the origin. This estimate is used in the recent work by the present author to establish the sharp criteria for recurrence and transience of the above-mentioned process.     

Visualizing recurrently migrating hydrogen in acetylene dication by intense ultrashort laser pulses

We demonstrate the visualization of ultrafast hydrogen migration in deuterated acetylene dication (C2D2{2+}) by employing the pump-probe Coulomb explosion imaging with sub-10-fs intense laser pulses (9 fs, 0.13 PW/cm{2}, 800 nm). It is shown, from the temporal evolution of the momenta of the fragment ions produced by the three-body explosion, C2D2{3+}-->D{+} + C{+} + CD{+}, that the migration proceeds in a recurrent manner: the deuterium atom first shifts from one carbon site to the other in a short time scale (approximately 90 fs) and then migrates back to the original carbon site by 280 fs, in competition with the molecular dissociation.     

On-line coupling of sequential injection extraction with restricted-access materials and post-column derivatization for sample clean-up and determination of propranolol in human plasma

The presented paper deals with a new methodology for direct determination of propranolol in human plasma. The methodology described is based on sequential injection analysis technique (SIA) coupled with solid phase extraction (SPE) column based on restricted access materials (RAM). Special RAM column containing 30 μm polymeric material—N-vinylacetamide copolymer was integrated into the sequential injection manifold. SIA–RAM system was used for selective retention of propranolol, while the plasma matrix components were eluted with two weak organic solutions to waste.

Due to the acid–basic and polarity properties of propranolol molecule and principles of reversed-phase chromatography, it was possible to retain propranolol on the N-vinylacetamide copolymer sorbent (Shodex MSpak PK-2A 30 μm (2 mm × 10 mm)). Centrifuged plasma samples were aspirated into the system and loaded onto the column using acetonitrile–water (5:95, v/v), pH 11.00, adjusted by triethylamine. The analyte was retained on the column while proteins contained in the sample were removed to waste. Interfering endogenous substances complicating detection were washed out by acetonitrile–water (15:85), pH 11.00 in the next step. The extracted analyte was eluted by means of tetrahydrofuran–water (25:75), pH 11.00 to the fluorescence detector (emission filter 385 nm). The whole procedure comprising sample pre-treatment, analyte detection and column reconditioning took about 15 min. The recoveries of propranolol from undiluted plasma were in the range 96.2–97.8% for three concentration levels of analyte. The proposed SIA–RAM method has been applied for direct determination of propranolol in human plasma.     

Fourier transform absorption spectra of H2O and H2O in the 3ν + δ and 4ν polyad region

Fourier transform absorption spectra of H₂¹⁸O-enriched and H₂¹⁷O-enriched water vapor in the 3ν + δ and 4ν polyad region have been analyzed. With the aid of theoretically calculated linelists, we have assigned 1014 lines attributed to H₂¹⁸O and 836 lines of 855 attributed to H₂¹⁷O. Seven new band origins are found for H₂¹⁷O and one for H₂¹⁸O.     

On some classes of unbounded operators

Hyponormality, normality and subnormality for unbounded operators on Hilbert space are investigated and quasi- similarity of such operators is discussed.     

Large deflection of a circular cantilever beam with uniformly distributed load

Summary In this paper, the bending of thin circular cantilever beam, convex downward, under a uniformly distributed load is discussedby the use of the Bernoulli-Euler equation. The solutions are obtained in the form of power series. Numerical results are also presented.The author wishes to thank Dr. S. Higuchi, Professor Emeritus of the Thoku University, for his suggestion and encouragement in the work. The author also wishes to thank Professor F. Numachi and Professor O. Tamate for their kind advice.