Synthesis of pyrroloquinolines as indole analogues of flavonols

7-Acetyl-4,6-dimethoxy-3-phenylindole 10 was converted into a range of 7-indolyl chalcones 13 by reaction with aryl aldehydes under basic conditions. Oxidation of the chalcones 13 with alkaline hydrogen peroxide gave the isolable epoxides 14, which were cyclized with further base treatment into the indole flavonols, or 5-hydroxy-6-oxopyrroloquinolines 15. The related compounds 25 and 26, examples of indole flavanones and flavones, respectively, were also synthesized. UV spectroscopic comparisons between flavonoids and indole flavonoids are discussed.     

Development and application of a novel immunoassay for measuring oxidative DNA damage in the environment

We developed a facile, cost-effective competitive binding assay for the analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in DNA, using a polyclonal rabbit antiserum raised against an 8-oxodGuo hapten coupled to bovine serum albumin and radiolabeled synthetic ligand containing multiple 8-oxodGuo residues. This radioimmunoassay (RIA) displays a high affinity for 8-oxodGuo in DNA, with a detection limit of approximately 1 adduct in 10(5) bases of DNA. 8-oxodGuo standards for RIA were quantified by high-performance liquid chromatography and electrochemical detection in DNA diluted in methylene blue and exposed to visible light. As an initial application we quantified 8-oxodGuo in dosimeters deployed at increasing depths in the Southern Ocean during the austral spring of the 1998 field season or at the surface at Palmer Station, Antarctica, throughout the 1999 field season. Cyclobutane pyrimidine dimers (CPD) were quantified using an established RIA. We found that the frequency of both photoproducts decreased with depth. However, CPD induction was attenuated at a faster rate than 8-oxodGuo, correlating with the differential attenuation of solar ultraviolet wavelengths in the water column. CPD induction was closely related with ultraviolet-B radiation (UVB) attenuation, whereas the lower attenuation of 8-oxodGuo suggests that oxidative damage is more closely related to ultraviolet-A radiation (UVA) irradiance. The ratio of 8-oxodGuo: CPD was also found to covary with changes in stratospheric ozone concentrations at Palmer Station. These data demonstrate the usefulness of these assays for environmental photobiology and the potential for their use in studying the relative impacts of UVB versus UVA, including ozone depletion events.     

Analysis of the Fenton-Karma model through an approximation by a one-dimensional map

The Fenton-Karma model is a simplification of complex ionic models of cardiac membrane that reproduces quantitatively many of the characteristics of heart cells; its behavior is simple enough to be understood analytically. In this paper, a map is derived that approximates the response of the Fenton-Karma model to stimulation in zero spatial dimensions. This map contains some amount of memory, describing the action potential duration as a function of the previous diastolic interval and the previous action potential duration. Results obtained from iteration of the map and numerical simulations of the Fenton-Karma model are in good agreement. In particular, the iterated map admits different types of solutions corresponding to various dynamical behavior of the cardiac cell, such as 1:1 and 2:1 patterns. (c) 2002 American Institute of Physics.     

Double-quantum dipolar recoupling at high magic-angle spinning rates

A full investigation of the possible homonuclear double-quantum recoupling sequences, based on the RN family of sequences with N < or = 20, is given. Several new RN sequences, R16(6)(5), R18(8)(5), and R18(10)(5), were applied at high magic-angle spinning rates and compared with theory. The R18(10)(5) technique can be used to recouple dipolar couplings at spinning rates up to 39 kHz, and the application of the sequence in an INADEQUATE experiment is shown for a spinning rate of 30 kHz.     

183-W, M(2) = 2.4 Yb:YAG Q-switched laser

We have fabricated a diode-array end-pumped Yb:YAG rod laser with output powers greater than 200 W cw and 195 W Q -switched at 5 kHz. At an output power of 183 W and a repetition rate of 5 kHz, the beam quality was measured to be M(2)=2.4 . The laser design incorporates a hollow lens duct to concentrate the diode pump light for delivery to the end of the laser rod while maintaining access to the laser beam. This configuration provides increased flexibility for the resonator design and permits the use of birefringence compensation in the cavity to yield polarized output with increased efficiency. Using the recently described birefringence compensation method of Clarkson et al. [in Conference on Lasers and Electro-Optics (Optical Society of America, Washington, D.C., 1998), paper CTuI3], we obtained 112 W of cw power with a polarized beam of M(2)=3.2.     

Oxidation of alcohols by transition metal complexes part V. Selective catalytic monoalkylation of arylacetonitriles by alcohols

Selective catalytic monoalkylation of arylacetonitriles by primary alcohols can be achieved at ≤ 100° using a catalyst prepared in situ from rhodium trichloride, triphenylphosphine and sodium carbonate. RuH₂(PPh₃)₄ is a more effective catalyst for this process.