全文获取类型
收费全文 | 401篇 |
免费 | 17篇 |
专业分类
化学 | 319篇 |
晶体学 | 1篇 |
力学 | 2篇 |
数学 | 50篇 |
物理学 | 46篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 9篇 |
2021年 | 13篇 |
2020年 | 17篇 |
2019年 | 13篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 15篇 |
2015年 | 10篇 |
2014年 | 14篇 |
2013年 | 25篇 |
2012年 | 28篇 |
2011年 | 37篇 |
2010年 | 13篇 |
2009年 | 16篇 |
2008年 | 32篇 |
2007年 | 24篇 |
2006年 | 25篇 |
2005年 | 28篇 |
2004年 | 21篇 |
2003年 | 16篇 |
2002年 | 12篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1997年 | 1篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1981年 | 3篇 |
1973年 | 2篇 |
排序方式: 共有418条查询结果,搜索用时 359 毫秒
31.
32.
33.
A Novel Streptavidin–luciferase Fusion Protein: Preparation,Properties and Application in Hybridization Analysis of DNA
下载免费PDF全文
![点击此处可从《Photochemistry and photobiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Daria V. Smirnova Maya Y. Rubtsova Vitaly G. Grigorenko Natalia N. Ugarova 《Photochemistry and photobiology》2017,93(2):541-547
A streptavidin–luciferase fusion protein comprising the thermostable mutant form of firefly luciferase Luciola mingrelica and minimal core streptavidin was constructed. The streptavidin–luciferase fusion was mainly produced in a tetrameric form with high luciferase and biotin‐binding activities. It was shown that fusion has the same Km values for ATP and luciferin and the bioluminescence spectra as initial luciferase. The linear dependence of the bioluminescence signal on the content of the fusion was observed within the range of 10?18–10?13 mol per well. Successful application of obtained fusion in a biospecific bioluminescence assay based on biotin–streptavidin interactions was demonstrated by the example of a specific DNA hybridization analysis. A DNA hybridization analysis for Escherichia coli cells identification was developed using unique for these cells gadB fragment encoding glutamate decarboxylase. The amplified biotinylated GadB fragments were hybridized with the immobilized oligonucleotide probes; then, the biotin in the DNA duplexes was detected using the streptavidin–luciferase fusion protein. To reach the high sensitivity of the assay, we optimized the conditions of the assay. It was shown that the use of Pluronic for plate modification resulted in a significant reduction in the DNA detection limit which finally was 0.4 ng per well. 相似文献
34.
Ladie Kimberly De La Cruz Xiaoxiao Yang Anna Menshikh Maya Brewer Wen Lu Minjia Wang Siming Wang Xingyue Ji Alyssa Cachuela Haichun Yang David Gallo Chalet Tan Leo Otterbein Mark de Caestecker Binghe Wang 《Chemical science》2021,12(31):10649
Carbon monoxide as an endogenous signaling molecule exhibits pharmacological efficacy in various animal models of organ injury. To address the difficulty in using CO gas as a therapeutic agent for widespread applications, we are interested in developing CO prodrugs through bioreversible caging of CO in an organic compound. Specifically, we have explored the decarboxylation–decarbonylation chemistry of 1,2-dicarbonyl compounds. Examination and optimization of factors favorable for maximal CO release under physiological conditions led to organic CO prodrugs using non-calorific sweeteners as leaving groups attached to the 1,2-dicarbonyl core. Attaching a leaving group with appropriate properties promotes the desired hydrolysis–decarboxylation–decarbonylation sequence of reactions that leads to CO generation. One such CO prodrug was selected to recapitulate the anti-inflammatory effects of CO against LPS-induced TNF-α production in cell culture studies. Oral administration in mice elevated COHb levels to the safe and efficacious levels established in various preclinical and clinical studies. Furthermore, its pharmacological efficacy was demonstrated in mouse models of acute kidney injury. These studies demonstrate the potential of these prodrugs with benign carriers as orally active CO-based therapeutics. This represents the very first example of orally active organic CO prodrugs with a benign carrier that is an FDA-approved sweetener with demonstrated safety profiles in vivo.1,2-Dicarbonyl compounds with FDA-approved sweeteners as leaving groups deliver CO for protection against acute kidney injury in mice. 相似文献
35.
We prove that the list chromatic index of a graph of maximum degree Δ and treewidth is Δ; and that the total chromatic number of a graph of maximum degree Δ and treewidth is . This improves results by Meeks and Scott. 相似文献
36.
Bulat Munavirov Oleg Gnezdilov Maya Rudakova Oleg N. Antzutkin Andrei Filippov 《Magnetic resonance in chemistry : MRC》2013,51(11):750-755
Polyanion‐coated lipid vesicles are proposed to have an appreciable potential for drug delivery because of their ability to control the permeability of lipid bilayers by environmental parameters such as pH and temperature. However, details of the interaction of this class of polymers with lipids and their mechanisms of induced permeability are still being debated. In this work, we applied 1H NOESY to study details of the interaction of polyacrylic acid (PAA) fractions of molecular weights 5 and 240 kDa with dimyristoylphosphatidylcholine vesicles. We showed that PAA of two different molecular masses modifies lipid bilayers increasing disorder and probability of close contact between polar and hydrophobic groups. PAA molecules adsorb near the interface of lipid bilayers but do not penetrate into the hydrophobic core of the bilayer and, thus, cannot participate in formation of transbilayer channels, proposed in earlier works. Increasing the molecular mass of PAA from 5 kDa to 240 kDa does not change the effect of PAA on the bilayer, although PAA240 forms a more compact structure (either intra‐molecular or inter‐molecular) and interacts more strongly with interface lipid protons. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
37.
Oluseye K. Onajole Maya M. Makatini Patrick Govender Thavendran Govender Glenn E. M. Maguire Hendrik G. Kruger 《Magnetic resonance in chemistry : MRC》2010,48(3):249-255
The synthesis and complete NMR elucidation of eight novel pentacycloundecane (PCU) derivatives are reported. These compounds are precursors in the synthesis of PCU‐based anti‐tuberculosis (TB) agents and potential human immunodeficiency virus (HIV) protease inhibitors. Two‐dimensional (2D) NMR techniques were used to assign the NMR spectra for these compounds. Substitution of the cage molecule at (C‐8/11) further complicates the assignment, since some of the substituted alkyl chain groups overlap with the cage proton signals. The side chain heteroatoms also introduce a rare through‐space deshielding effect to some of the carbon atoms of the cage skeleton. Ring strain in the rigid cage skeleton appears to induce drastic electronic changes in some parts of the cage framework. This observation is more dramatic for the C‐4 methylene group of the cage diols and the cage ethers. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
38.
Maya Jakobine Stein 《Journal of Graph Theory》2007,54(4):331-349
A theorem of Mader states that highly connected subgraphs can be forced in finite graphs by assuming a high minimum degree. We extend this result to infinite graphs. Here, it is necessary to require not only high degree for the vertices but also high vertex‐degree (or multiplicity) for the ends of the graph, that is, a large number of disjoint rays in each end. We give a lower bound on the degree of vertices and the vertex‐degree of the ends which is quadratic in k, the connectedness of the desired subgraph. In fact, this is not far from best possible: we exhibit a family of graphs with a degree of order 2k at the vertices and a vertex‐degree of order k log k at the ends which have no k‐connected subgraphs. Furthermore, if in addition to the high degrees at the vertices, we only require high edge‐degree for the ends (which is defined as the maximum number of edge‐disjoint rays in an end), Mader's theorem does not extend to infinite graphs, not even to locally finite ones. We give a counterexample in this respect. But, assuming a lower bound of at least 2k for the edge‐degree at the ends and the degree at the vertices does suffice to ensure the existence (k + 1)‐edge‐connected subgraphs in arbitrary graphs. © 2006 Wiley Periodicals, Inc. J Graph Theory 54: 331–349, 2007 相似文献
39.
An extended data set of extreme ultraviolet images of the solar corona provided by the SOHO spacecraft is analyzed using statistical methods common to studies of self-organized criticality (SOC) and intermittent turbulence (IT). The data exhibit simultaneous hallmarks of both regimes: namely, power-law avalanche statistics as well as multiscaling of structure functions for spatial activity. This implies that both SOC and IT may be manifestations of a single complex dynamical process entangling avalanches of magnetic energy dissipation with turbulent particle flows. 相似文献
40.
Textile/Metal–Organic‐Framework Composites as Self‐Detoxifying Filters for Chemical‐Warfare Agents
下载免费PDF全文
![点击此处可从《Angewandte Chemie (International ed. in English)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Elena López‐Maya Dr. Carmen Montoro Dr. L. Marleny Rodríguez‐Albelo Dr. Salvador D. Aznar Cervantes Dr. A. Abel Lozano‐Pérez Prof. José Luis Cenís Dr. Elisa Barea Prof. Jorge A. R. Navarro 《Angewandte Chemie (International ed. in English)》2015,54(23):6790-6794
The current technology of air‐filtration materials for protection against highly toxic chemicals, that is, chemical‐warfare agents, is mainly based on the broad and effective adsorptive properties of hydrophobic activated carbons. However, adsorption does not prevent these materials from behaving as secondary emitters once they are contaminated. Thus, the development of efficient self‐cleaning filters is of high interest. Herein, we report how we can take advantage of the improved phosphotriesterase catalytic activity of lithium alkoxide doped zirconium(IV) metal–organic framework (MOF) materials to develop advanced self‐detoxifying adsorbents of chemical‐warfare agents containing hydrolysable P? F, P? O, and C? Cl bonds. Moreover, we also show that it is possible to integrate these materials onto textiles, thereby combining air‐permeation properties of the textiles with the self‐detoxifying properties of the MOF material. 相似文献