Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR

A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5'-end, the 3'-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective with a wide range of fluorescent dyes. The anthraquinone moiety increases the melting temperature of DNA duplexes, thus allowing shorter, more discriminatory probes to be used. The quencher has been used in Scorpion primers and TaqMan probes for human DNA sequence recognition and mutation detection.     

Synthesis of certain 5-substituted thieno[2,3-b]thiophene-2-sulfonamides and thieno[2,3-b]thiophene-2-sulfonamide 6,6-dioxides

A series of the title compounds were prepared for evaluation as inhibitors of carbonic anhydrase II. Oxidation of 5-substituted thieno[2,3-b]thiophene-2-sulfonamides provided the first examples of thiophene[2,3-b]thiophene-2-sulfonarnide 6,6-dioxides. These cyclic vinyl sulfones readily underwent addi tion to give predominately that 4,5-cis addition product.     

Uranium oligomerization in chloride-based high temperature melts: in situ XAS studies

In situ EXAFS spectroscopic studies of uranium compounds in high temperature alkali chloride melts indicate the presence of oligomeric species. An investigation into UCl(3) and UCl(4) dissolved in LiCl reveals long range ordering of uranium atoms in the molten state which is not maintained on quenching. Studies of uranium dioxide dissolved in LiCl-KCl eutectic with HCl exhibit long range ordering in both molten and quenched states, and the EXAFS data can be modeled using multiple coordination shells.     

A convenient precursor to 3,4-didehydropyridine

The preparation of 3-(3,3-dimethyltriazen-l-yl)pyridine-4-carboxylic acid (5), a convenient shelf-stable precursor to 3,4-didehydropyridine, is described.     

Morphology transformation of thermosensitive metronidazol by spray freeze-drying

Journal of Thermal Analysis and Calorimetry - Spray freeze-drying (SFD) is an effective method for reducing the size of thermosensitive organic substances that are sparingly soluble in water....     

Copper-linked hexaniobate lindqvist clusters-variations on a theme

Four new one-dimensional materials and one dimer complex based on the linkage of [Nb6O19] clusters and [CuLx] (L=ethylenediamine (en), NH3, H2O) assemble under ambient conditions. These phases include the following: Rb4[Cu(en)2(H2O)2]3[(Nb6O19H2)2Cu(en)2].24H2O (1), space group P; [Cu(en)2(H2O)2]2[(Nb6O19H2)Cu(en)2].14H2O (2), space group P; Rb2[Cu(NH3)2(H2O)4][Cu(NH3)4(H2O)2]2{[Nb6O19][Cu(NH3)]2}(2).6H2O (3), space group P; {[Nb6O19][Cu(NH3)2(H2O)]2[Cu(H2O)4]2}.3H2O (4), space group P2/n; and {[Nb6O19][Cu(NH3)2(H2O)]2[Cu(H2O)4]2} (5), space group C2/m. All structures have been solved by single-crystal methods, and compounds 1-5 were characterized by thermogravimetric analysis, Fourier transform IR, chemical analysis, and magnetic measurements. It has been demonstrated that the conformation, charge, and geometry of the [Nb6O19]-[CuLx] chains can be modulated by varying the type and amount of the [CuLx]2+ species. The charge balance is provided by mixed Rb+/[CuLx]2+ or [CuLx]2+ cations only for structures 1-3, whereas 4 and 5 are neutral chains with no counterions. There are weak antiferromagnetic Cu2+-Cu2+ interactions in all phases. Compounds 2-5 represent the first examples in which the [Nb6O19] Lindqvist ion forms extended solids rather than dimers or decorated monomers when reacted with transition-metal, cationic complexes.     

Supremizers of inner <Emphasis Type="Italic">γ</Emphasis>-convex functions

A real-valued function f defined on a convex subset D of some normed linear space is said to be inner γ-convex w.r.t. some fixed roughness degree γ > 0 if there is a such that holds for all satisfying ||x ₀ − x ₁|| = νγ and . This kind of roughly generalized convex functions is introduced in order to get some properties similar to those of convex functions relative to their supremum. In this paper, numerous properties of their supremizers are given, i.e., of such satisfying lim . For instance, if an upper bounded and inner γ-convex function, which is defined on a convex and bounded subset D of some inner product space, has supremizers, then there exists a supremizer lying on the boundary of D relative to aff D or at a γ-extreme point of D, and if D is open relative to aff D or if dim D ≤ 2 then there is certainly a supremizer at a γ-extreme point of D. Another example is: if D is an affine set and is inner γ-convex and bounded above, then for all , and if 2 ≤ dim D < ∞ then each is a supremizer of f.      

Representation of Bounded Convex Sets By Rational Convex Hull Of Its Gamma-Extreme Points

H. Minkowski proved C= convext C whenever C is a compact convex subset of a finite-dimensional linear space. If C is bounded but not closed, this representation does not hold anymore. In this case, we introduce the set of so-called γ-extreme points extγC of C and show C = convext γ C = raco extγ C, where raco M denotes the rational convex hull of M.