Spectroscopic properties of the photoproducts of pyridoxal-5′-P irradiation: Catalytic site recognition of ribonuclease A

Photoproducts of pyridoxal-5-P, i.e., 4-pyridoxic-5-P and bis-pyridoxal-5-P, have been studied by spectroscopic methods. The spectroscopic properties of bis-pyridoxal-5-P (bis-PLP) resemble those of pyridoxal-5-P (PLP) under similar experimental conditions. The coupling of methylen hydrogens to the phosphorus atom has been shown by NMR spectroscopy. The singlet in the³¹P-NMR spectra and the triplet in¹H-undecoupled experiments confirm the presence of the phosphate group in the 5 position of the structure of the vitamin. The effect of pH and solvent composition on the relative distribution of species of bis-pyridoxine-5-P (bis-PNP) has been investigated by absorption and fluorescence spectroscopy. The acid-base dissociation of the phosphate group is easily detected by emission spectroscopy. Bis-PNP and bis-PLP bind to the enzyme RNase A and they behave as competitive inhibitors with respect to the substrate cytidine-2-3-cyclic phosphate. The natural forms of vitamin B₆, pyridoxine, and pyridoxine-5-P have no effect on the catalytic activity of the protein. Experimental evidence derived from fluorescence and inhibition experiments is consistent with the hypothesis that bis-PNP recognizes the catalytic site of RNase A.     

Effect of acetate on ferrihydrite crystallization

Mössbauer spectroscopy was used to follow the aging of ferrihydrite with different amounts of acetate, to achieve more crystalline products. Mössbauer spectra of fresh samples did not show any magnetic components. After two years aging, the solids presented crystalline fractions. Hematite formation was inhibited as acetate content increased in solids. For an acetate/iron molar ratio equal to 0.63 goethite was formed instead of hematite. X-ray diffraction confirmed these results. This work shows that synthetic samples can be used for elucidating the anti-hematitic effect of organic matter already detected in soils.     

Multipliers of spherical harmonics and energy of measures on the sphere

We consider the operator,f(Δ) for Δ the Laplacian, on spaces of measures on the sphere inR ^d , show how to determine a family of approximating kernels for this operator assuming that certain technical conditions are satisfied, and give estimates for theL²-norm off(Δ)μ in terms of the energy of the measure μ. We derive a formula, analogous to the classical formula relating the energy of a measure onR ^d with its Fourier transform, comparing the energy of a measure on the sphere with the size of its spherical harmonics. An application is given to pluriharmonic measures.     

Operator–valued Fourier multiplier theorems on Besov spaces

We investigate analytical properties of a measure geometric Laplacian which is given as the second derivative w.r.t. two atomless finite Borel measures μ and ν with compact supports supp μ ? supp ν on the real line. This class of operators includes a generalization of the well‐known Sturm‐Liouville operator as well as of the measure geometric Laplacian given by . We obtain for this differential operator under both Dirichlet and Neumann boundary conditions similar properties as known in the classical Lebesgue case for the euclidean Laplacian like Gauß‐Green‐formula, inversion formula, compactness of the resolvent and its kernel representation w.r.t. the corresponding Green function. Finally we prove nuclearity of the resolvent and give two representations of its trace.     

An interior estimate for a nonlinear parabolic equation

In this paper the derivatives of the solution of an initial boundary value problem for a nonlinear uniformly parabolic equation in the interior with the total variation of the boundary data and the L^∞-norm of the initial condition are estimated.     

Canonical connections with an algebraic curvature tensor field on naturally reductive spaces

We study Ambrose-Singer connections with an algebraic curvature tensor on simply connected manifolds carrying a homogeneous Riemannian structure of class ₃ in the classification given by F. Tricerri and L. Vanhecke.This work was partially supported by MURST.     

Polygon triangulation inO(n log logn) time with simple data structures

We give a newO(n log logn)-time deterministic algorithm for triangulating simplen-vertex polygons, which avoids the use of complicated data structures. In addition, for polygons whose vertices have integer coordinates of polynomially bounded size, the algorithm can be modified to run inO(n log*n) time. The major new techniques employed are the efficient location of horizontal visibility edges that partition the interior of the polygon into regions of approximately equal size, and a linear-time algorithm for obtaining the horizontal visibility partition of a subchain of a polygonal chain, from the horizontal visibility partition of the entire chain. The latter technique has other interesting applications, including a linear-time algorithm to convert a Steiner triangulation of a polygon into a true triangulation.This research was partially supported by the following grants: NSERC 583584, NSERC 580485, ONR-N00014-87-0467, and by DIMACS, an NSF Science and Technology Center (NSF-STC88-09648).     

Elliptic Equations with Degenerate Coercivity： Gradient Regularity

In this paper, we prove higher integrability results for the gradient of the solutions of some elliptic equations with degenerate coercivity whose prototype is where for example, a(x,u)=(1+|u|)^−θ with θ ∈ (0,1). We study the same problem for minima of functionals closely related to the previous equation.     

Asymptotic behavior of solutions of variational equations

In questo articolo si considerano equazioni differenziali ordinarie del secondo ordine della forma $$\{ A(u')u'\} ' + \delta (r)A(u')u' + f(r,u) = 0,$$ dove cioè la nonlinearità è presente sia nella variabile soluzioneu che nella sua derivata. Si forniscono proprietà di monotonia, oscillazione e un accurato studio del comportamento asintotico all’ infinito delle soluzioni, quandoA, δ,f hanno crescite asintotiche di tipo algebrico.