排序方式: 共有72条查询结果,搜索用时 30 毫秒
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A novel amperometric glucose biosensor based on layer‐by‐layer (LbL) electrostatic adsorption of glucose oxidase (GOx) and dendrimer‐encapsulated Pt nanoparticles (Pt‐DENs) on multiwalled carbon nanotubes (CNTs) was described. Anionic GOx was immobilized on the negatively charged CNTs surface by alternatively assembling a cationic Pt‐DENs layer and an anionic GOx layer. Transmission electron microscopy images and ζ‐potentials proved the formation of layer‐by‐layer nanostructures on carboxyl‐functionalized CNTs. LbL technique provided a favorable microenvironment to keep the bioactivity of GOx and prevent enzyme molecule leakage. The excellent electrocatalytic activity of CNTs and Pt‐DENs toward H2O2 and special three‐dimensional structure of the enzyme electrode resulted in good characteristics such as a low detection limit of 2.5 μM, a wide linear range of 5 μM–0.65 mM, a short response time (within 5 s), and high sensitivity (30.64 μA mM?1 cm?2) and stability (80% remains after 30 days). 相似文献
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Since serious fire occurred frequently in recent years, fire safety of high-rise building hasattracted extensive attention. A National Basic Research Program (973 program) of China has been setup by Ministry of Science and Technology (MOST) of China in 2012 to meet the research requirementsof fire safety in high-rise buildings. This paper reviews the current state of art of research onfire dynamics of high-rise buildings, including the up-to-date progress of this project. Thefollowing three subjects on fire dynamics of high-rise buildings are addressed in this review: theejected flame and fire plumebehavior over facade out ofthe compartment window, the flame spread behavior over facade thermal insulation materials, and the buoyancy-driven smoke transportationcharacteristicsalong long vertical channels in high-rise buildings. Prospective future works are discussed andsummarized. 相似文献
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An enhanced amperometric biosensor based on incorporating one kind of unique nanobiocomposite as dopant within an electropolymerized polypyrrole film has been investigated. The nanobiocomposite was synthesized by self-assembling glutamate dehydrogenase (GLDH) and poly(amidoamine) dendrimer-encapsulated platinum nanoparticles (Pt-DENs) onto multiwall carbon nanotubes (CNTs). ζ-Potentials and high-resolution transmission electron microscopy (HRTEM) confirmed the uniform growth of the layer-by-layer nanostructures onto the carboxyl-functionalized CNTs. The size of Pt nanoparticles is approximately 3 nm. The (GLDH/Pt-DENs)n/CNTs/Ppy hybrid film was obtained by electropolymerization of pyrrole onto glassy carbon electrodes and characterized with scanning electron microscopy (SEM), cyclic voltammetry (CV) and other electrochemical measurements. All methods indicated that the (GLDH/Pt-DENs)n/CNTs nanobiocomposites were entrapped within the porous polypyrrole film and resulted in a hybrid film that showed a high electrocatalytic ability toward the oxidation of glutamate at a potential 0.2 V versus Ag/AgCl. The biosensor shows performance characteristics with high sensitivity (51.48 μA mM−1), rapid response (within 3 s), low detection limit (about 10 nM), low level of interference and excellent reproducibility and stability. 相似文献
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This review presents a comprehensive survey of recent progress on electrochemiluminescence (ECL) detection coupled with capillary
electrophoresis (CE). The fundamental theories involved in CE-ECL, e.g., the mechanism involving both coreactant-based and
inhibitor-based ECL, as well as the possible analytes to be detected by CE-ECL are summarized. Different schemes for the construction
of CE-ECL apparatus, including methods for preparing the working electrode, approaches for addition of ECL reagents, ways
to fabricate electrical decouplers, and factors affecting ECL efficiency are reviewed. Discussion of the literature related
to the application of CE-ECL from January 2005 to September 2010 is sorted by the corresponding analyte matrixes, namely,
the standard solution, urine, serum and plasma, and other matrixes. Finally, possible trends for CE-ECL in the near future
are discussed. 相似文献
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Z Wang L Tang LH Tan J Li Y Lu 《Angewandte Chemie (International ed. in English)》2012,51(36):9078-9082
DNA is in control: Different combinations of DNA nucleotides can control the shape and surface roughness of gold nanoparticles during their synthesis. These nanoparticles were synthesized in the presence of either homogenous oligonucleotides or mixed-base oligonucleotides using gold nanoprisms as seeds. The effect of the individual DNA bases and their combinations on shape control are shown in the figure. 相似文献
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Wei X Lin W Ma N Luo F Lin Z Guo L Qiu B Chen G 《Chemical communications (Cambridge, England)》2012,48(49):6184-6186
Based on the specific folic acid-folate receptor (FA-FR) interaction, macromolecular FR can bind with FA-linked DNA-small molecule chimeras, which can prevent enzymolysis by exonuclease III (Exo III), enabling a novel fluorescence biosensor for FR to be developed using quinaldine red as a G-quadruplex-binding probe. 相似文献