Synthesis of new 2-aryl-6-styryl-2,3-dihydropyridin-4(1H)-one derivatives from curcuminoids

Series of new 2-aryl-6-styryl-2,3-dihydropyridin-4(1H)-one derivatives were synthesized in acceptable to good yields by treatment of the curcuminoids with aqueous ammonia,the mechanism was proposed.By modification of the primary products,total 13 new compounds were obtained.The structures of all products were elucidated by spectroscopy analysis including HR-MS,~1H NMR and ~(13)C NMR.     

Synthesis, characterization and properties of β-octamolybdate-supported transition metal complexes with mixed ligands: [Cu(2,2′-bipy)(C6H5NO2)(H2O)]2[Mo8O26]·5H2O

A novel organic–inorganic hybrid compound [Cu(2,2′-bipy)(C₆H₅NO₂)(H₂O)]₂[Mo₈O₂₆]·5H₂O (1), (2,2′-bipy = 2,2′-bipyridine; C₆H₅NO₂ = pyridine-4-carboxylic acid) has been prepared and characterized by elemental analyses, IR spectrum, thermal stability analysis, and single-crystal X-ray diffraction. Compound 1 is a discrete cluster, constructed from β-[Mo₈O₂₆]⁴⁻ subunits covalently bonded to two [Cu(2,2′- bipy)(C₆H₅NO₂)(H₂O)]₂⁴⁺ coordination cations via terminal oxo groups that connect one molybdenum site. A 3D supramolecular network is further formed by extensive hydrogen bonding interactions and π–π interactions of the 2,2′-bipy and pyridine-4-carboxylic acid ligands. EPR and magnetic susceptibility studies have been used to elucidate the electronic properties of the Cu²⁺ centres, and the results are in good agreement with the structural features of the compound.     

成都经济生态区大气降尘中镉赋存形态的研究

采用Tessier连续提取法对成都经济生态区不同区域的24个大气降尘样中镉的赋存形态进行了研究。研究结果表明,大气降尘中的镉主要以残留态形态存在;各形态镉在总镉中所占百分比含量由大到小排列顺序依次是:残留态,碳酸盐结合态,交换态,铁锰结合态,有机结合态;交换态镉、碳酸盐结合态镉这两种具有较高生物有效性的形态镉在总镉中所占的平均百分比含量分别是6.420%和8.917%;铁锰氧化物结合态、有机结合态、残留态镉这三种形态镉生物有效性很低,分别在总镉中所占的平均百分比含量分别是3.419%、2.365%和78.907%。通过分析可得出,汽油和煤的燃烧以及工厂排放镉能加大城市大气降尘的镉污染。     

适用于RTM成型聚酰亚胺材料研究进展

综述了适用于RTM成型耐高温聚酰亚胺材料的研究进展，主要包括降冰片烯酸酐（NA）封端的PMR聚酰亚胺树脂和使用苯乙炔基封端剂合成的酰亚胺低聚物，在这其中采用苯乙炔基封端剂合成的酰亚胺低聚物具有低的熔体粘度和良好的熔体稳定性，固化交联后的聚合物及树脂基复合材料具有良好的热性能和力学性能。本文介绍了上述聚合物化学合成、结构与性能之间的关系，并对适用于RTM成型耐高温聚酰亚胺材料的应用进行了简单介绍。     

湄洲湾海域环境质量评价

根据湄洲湾海域2005年丰、平、枯三个水期的海水监测及2005年9月的湄洲湾表层沉积物监测数据对湄洲湾的环境质量进行评价。结果显示,湄洲湾海域水质综合指数(WQI)全年平均为0.69,除了F1站位枯水期为1.04,轻度污染,其余的均小于1.0,全年综合评价为清洁,但受气候及水文条件的影响,丰水期和枯水期有两个站位呈富营养化状态;表层沉积物综合质量指标平均值为0.52,综合评价为清洁,但铜、砷、镉的单因子污染指数平均值介于0.5~1.0之间,说明表层沉积物已被这三个因子玷污。     

有机磷农药酶生物传感器研究进展

酶生物传感器（EBS）以简单、廉价、易于微型化等优势成了有机磷农药（OPs）传统分析方法的最佳替代品。本文从识别OPs的酶及识别机理、电化学EBS、酶的固定化技术、高分子材料的酶固定载体不同角度综述了有机磷农药酶生物传感器研究近况,并重点介绍了一次性丝网印刷酶电极。     

血红素蛋白的分子设计新趋向

综述了近年来有关血红素蛋白分子设计所出现的新趋向, 包括氨基酸选择突变与血红素修饰相结合,非天然辅基的引入,非天然氨基酸的引入,辅基与蛋白肽链的共价结合,以及全新血红素蛋白的设计与构建5个方面。这些新发展趋向对研究金属蛋白的结构-功能关系提供了重要的信息,同时也为这些理性设计的新颖金属蛋白分子拓宽了其在生物化学、生物工程和药学上的应用。     

纳米羟基磷灰石制备及其对溶液中苯酚吸附的优化设计

采用化学沉淀法制备得到纳米羟基磷灰石(n-HAp)粉体,研究了n-HAp粉体对水溶液中苯酚的吸附性能,并初步探讨了其在粉体上的吸附机理,在低浓度(5~30mg/L)时的吸附符合Freundlich等温吸附模型。实验结果表明,n-HAp粉体对苯酚具有较好的吸附效果,2h可基本达到吸附平衡。利用正交设计实验探讨了粉体煅烧温度、吸附温度、吸附时间、溶液pH等因素对吸附效果的影响。正交实验结果统计分析表明,各种因素对吸附的影响程度依次为:溶液pH>煅烧温度>振荡温度>振荡时间。pH对吸附性能的影响最明显,强酸和强碱环境能有效提高n-HAp对苯酚的吸附量。     

Genome mining in Streptomyces coelicolor: molecular cloning and characterization of a new sesquiterpene synthase

The terpene synthase encoded by the SCO5222 (SC7E4.19) gene of Streptomyces coelicolor was cloned by PCR and expressed in Escherichia coli as an N-terminal-His6-tag protein. Incubation of the recombinant protein, SCO5222p, with farnesyl diphosphate (1, FPP) in the presence of Mg(II) gave a new sesquiterpene, (+)-epi-isozizaene (2), whose structure and stereochemistry were determined by a combination of 1H, 13C, COSY, HMQC, HMBC, and NOESY NMR. The steady-state kinetic parameters were kcat 0.049 +/- 0.001 s-1 and a Km (FPP) of 147 +/- 14 nM. Individual incubations of recombinant epi-isozizaene synthase with [1,1-2H2]FPP (1a), (1R)-[1-2H]-FPP (1b), and (1S)-[1-2H]-FPP (1c) and NMR analysis of the resulting deuterated epi-isozizaenes supported an isomerization-cyclization-rearrangement mechanism involving the intermediacy of (3R)-nerolidyl diphosphate (3).     

Deposition of DNA-functionalized gold nanospheres into nanoporous surfaces

We report the deposition of DNA-conjugated gold nanospheres into arrays of surface nanopores obtained from hexagonally ordered thin polystyrene-b-poly(methyl methacrylate) (PS-b-PMMA) diblock copolymer films on silicon. The deposition occurs spontaneously from aqueous solution and is driven by either electrostatic interactions or specific DNA hybridization events between the DNA nanospheres and the surface nanopores. To mitigate this spontaneous deposition, we have chemically modified the nanopores with either positively charged aminosilanes or oligonucleotide probe sequences. The deposition of DNA nanospheres into the surface nanopores was characterized by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). We have observed preferential immobilization of individual DNA nanospheres within the nanopores, based on the size matching between the two entities. The inclusion density and selectivity of DNA nanosphere deposition into the surface nanopores was found to depend predominantly on the methods through which the nanoporous surfaces were prepared and chemically functionalized.