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Dubbs T Albuquerque IF Bondar NF Carrigan R Chen D Cooper PS Lisheng D Denisov AS Dobrovolsky AV Endler AM Escobar CO Foucher M Golovtsov VL Gottschalk H Gouffon P Grachev VT Khanzadeev AV Kubantsev MA Kuropatkin NP Lach J Pengfei L Chengze L Yunshan L Luksys M Mahon JR McCliment E Morelos A Newsom C Pommot Maia MC Samsonov VM Schegelsky VA Huanzhang S Smith VJ Fukun T Terentyev NK Timm S Tkatch II Uvarov LN Vorobyov AA Jie Y Wenheng Z Shuchen Z Yuanyuan Z 《Physical review letters》1994,72(6):808-811
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Linda M. M. Weigang Dieter Langosch Thomas Letzel 《Rapid communications in mass spectrometry : RCM》2008,22(24):4089-4097
Specific helix oligomerization between transmembrane segments (TMSs) is often promoted by motifs like GxxxG. Disruption of this motif in the transmembrane segments of vesicular stomatitis virus G‐protein and of glycophorin A results in a reduced dimerization level studied by in vivo systems like ToxR. This paper reports the influence of sequence motifs like GxxxG in solution and the gas phase. The transmembrane segments may behave differently in the gas and liquid phase, because of the absence of surrounding solvent molecules in the gas phase. Comparison of experiments depending on peptide properties performed in the gas and liquid phase discloses that the peptides retain ‘some memory’ of their liquid‐phase structure in the gas phase. A direct correlation has been found between helicity in solution as determined by circular dichroism and dimerization in the gas phase monitored by electrospray mass spectrometry. These results show that a proper folding in solution is required for oligomerization. On the other hand, sequence‐specific oligomerization depending on the GxxxG motif was not observed with the mass spectrometric detection. Further on, neither concentration‐dependent complex studies nor studies regarding complex stability in the gas phase – via collision‐induced dissociation (CID) – led to sequence‐specific differences. Finally, the findings show that in mass spectrometric measurements noncovalent interactions of studied TMSs is rather more dependent on the secondary structure and proper folding than on their primary structure. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
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The numerical solution of a possible inconsistent system oflinear inequalities in the l1 sense is considered. The non-differentiablel1 norm minimization problem is approximated by a piecewisequadratic Huber smooth function. A continuation algorithm isdesigned to find an l1 solution of the inequality system. Inthe case where the linear inequality system is consistent, asolution is obtained by solving any smoothed problem. Otherwise,the algorithm is shown to terminate in a finite number of iterations.We also consider an alternative smoothing scheme which sharessimilar properties with the first one, but results in an improvedcomputational performance of the continuation algorithm on inconsistentsystems. Numerical experiments are conducted to test the efficiencyof the algorithm. 相似文献