Eine neue volumetrische Methode zur Bestimmung der salpetrigen S?ure

Ohne Zusammenfassung     

Ueber die Destillation von Formaldehyd

Ohne Zusammenfassung     

Zur Untersuchung von Zuckersirup

Ohne Zusammenfassung     

SOLUBILIZATION OF PHOTOSYNTHETIC PIGMENT-PROTEIN COMPLEXES

Studies utilizing fractionation of photosynthetic pigment-protein complexes from the chloroplast thylakoid membrane often employ dodecylsulfate at a concentration of 10 mg mL^?1 to disrupt membrane structure prior to electrophoretic fractionation of the complexes. We investigated the effect of varying dodecylsulfate concentration on the solution/air interfacial surface tension in the absence and presence of the same concentrations of thylakoid membranes used by four different fractionation systems that have been commonly employed to fractionate photosynthetic pigment-protein complexes. Concentrations of dodecylsulfate in the range5–10 mg mL^?1, normally utilized to treat thylakoids prior to fractionation, were effective in reducing the interfacial surface tension to levels equivalent to control solutions without added thylakoid membranes. However, thylakoid membranes treated with these concentrations of dodecylsulfate are not resolved into discrete pigment-protein complexes when subjected to electrophoresis on an agarose gel, and do not produce significant amounts of pigment-containing complexes with a molecular size < 100 000 as measured by filtration with size-exclusion membranes. We conclude that many surfactant systems empirically developed to fractionate photosynthetic pigment-protein complexes may not fully solubilize the complexes prior to the electrophoretic step.     

Fortran codes for the correlation functions of hard sphere fluids

Our Fortran codes for hard sphere fluids and their mixtures for the correlation functions that arise from the Percus–Yevick theory and the Verlet–Weis semi-empirical correction have proven useful during a period of nearly four decades and continue to be useful. In order to make these codes even more widely available, a brief summary is presented here and listings of these codes are given in the electronically accessible Supplementary Material to this paper.     

A consistency check for estimating truncation error due to upstream differencing

A simple method is developed for checking the consistency—i.e., the degree of self-consistent numerical accuracy—of fluid dynamic computations which use upstream differencing for the convection terms. By applying the method to computed results, a quantitative estimate of the size of the first-order truncation error can be made, thus obviating the need for grid-dependence tests based on successive grid refinement. Alternatively, the method can be used to determine the grid size appropriate to an acceptable range of truncation error. In regions of relatively small velocity gradient, a direct consistency check can be achieved by applying a straightforward graphical procedure to computed results. The same graphical construction can be used in the general case to make an adequate first-order consistency check on the convective flux computation. The method is particularly useful in rationalizing empirical turning procedures used to calibrate upstream-difference numerical models in terms of measured results.     

A second generation dendrimer incorporating nine S2N2-donor macrocycles and its palladium(II) complex

A new second generation dendrimer incorporating nine S2N2-donor macrocyclic units that bind nine Pd(II) cations is reported.     

A New existence and Uniqueness Proof for the O'nan Group

We give a new short proof of the existence and uniqueness ofthe O'Nan sporadic simple group. The only other uniqueness proofforms the main part of Andrilli's Ph.D. thesis [1], and hasnot otherwise been published. In the process, we derive a concisepresentation for the O'Nan group.     

Study of the primary structure of recombinant tissue plasminogen activator by reversed-phase high-performance liquid chromatographic tryptic mapping

Two high-resolution tryptic maps have been developed for recombinant tissue plasminogen activator (rt-PA) that separate the expected 51 tryptic peptides. The trypsin digestion was performed after reduction and S-carboxymethylation of the protein. The high-performance liquid chromatographic separation of the tryptic peptides used a Nova-Pak C18 (5 microns) column with a mobile phase that contained 0.1% aqueous trifluoroacetic acid (TFA) or 50 mM sodium phosphate (pH 2.85) and a linear gradient of acetonitrile. A TFA solvent system was also used for re-purification and for characterization of the peptides isolated from the phosphate-based separation. All of the isolated peptides had compositions consistent with the sequence proposed for rt-PA. The identities of the glycopeptides were confirmed by lectin chromatography on concanavalin A-Sepharose. The mixture of tryptic peptides was also treated with endo-beta-N-acetylglucosaminidase H and peptide:N-glycosidase F to locate the position of either high mannose or complex oligosaccharides. These studies demonstrated that a high mannose oligosaccharide is attached to Asn-117 while complex carbohydrate side-chains are attached to Asn-184 and Asn-448. The residue Asn-184 is the site of optional glycosylation that results in the formation of two rt-PA variants that contain either two or three oligosaccharides.