Estimation of trace elements in some medicinal plants used in anti‐cancer drugs by PIXE

A trace elemental analysis was carried out in various parts of 12 anticancer medicinal plants, using the PIXE (particle‐induced X‐ray emission) technique. A 3‐MeV proton beam was used to excite the samples, and spectra were recorded using a Si (Li) detector. Data analysis was done using the GUPIX software. The elements Cl, K, Ca, Ti, V, Mn, Fe, Ni, Cu, Zn, Br and Sr were identified, and their concentrations estimated. The results of the present study provide justification for the usage of these medicinal plants in the development of anticancer drugs. Copyright © 2012 John Wiley & Sons, Ltd.     

Cross Polarization for Improved Digital Image Correlation

Digital image correlation (DIC) is a surface deformation measurement technique for which accuracy and precision are sensitive to image quality. This work presents cross polarization, the use of orthogonal linear polarizers on light source(s) and camera(s), as an effective method for improving optical DIC measurements. The benefits of cross polarization are characterized through quantitative and statistical comparisons from two experiments: rigid body translation of a flat sample and uniaxial tension of a superelastic shape-memory alloy (SMA). In both experiments, cross polarization eliminated saturated pixels that degrade DIC measurements, and increased image contrast, which enabled higher spatial precision by using smaller subsets. Subset sizes are usually optimized for correlation confidence interval (typically with subsets of 21×21 px or larger), but can be decreased to achieve the highest possible spatial precision at the expense of increased correlation confidence intervals. Smaller subset sizes (such as 9×9 px) require better images to maintain correlation within error thresholds. By comparing DIC results from a uniaxial SMA tension test with unpolarized and cross-polarized images, we show that for 9×9 px subsets, the loss of valid DIC data points was reduced almost ten-fold with cross polarization. The only disadvantage we see to cross polarization is the decrease in specimen illumination due to transmission losses through the polarizers, which can easily be accommodated with sufficiently intense light sources. With the installation of relatively inexpensive linear polarizing filters, an optimum optical DIC setup can provide even better DIC measurements by delivering images without saturated pixels and with higher contrast for increased DIC spatial precision.     

Determination of acephate in human urine

Acephate is a commonly used organophosphate insecticide applied on agricultural crops and in residential communities. Because very little acephate is metabolized prior to excretion, the parent pesticide compound can be measured in human urine. The residue method must be sensitive enough to determine human exposure and potential health risk for both agricultural workers and their families who may be exposed by pesticide drift or by inadvertent carry-home residues. A reliable and sensitive method was developed to measure acephate concentrations in human urine. Urine was diluted with water and acetone, adjusted to a neutral pH, and partitioned twice in acetone-methylene chloride (1 + 1, v/v), with NaCl added to aid separation. The solvent-reduced organic phase extracts were clarified by activated charcoal solid-phase extraction and then adjusted to a final volume with the addition of a D-xylose analyte protectant solution to reduce matrix enhancement effects. Acephate concentrations in urine were determined by gas chromatography using pulsed flame photometric detection. The method limit of detection was established at 2 microg/L, with a method limit of quantitation of 10 microg/L. The average recovery from urine fortified with 10-500 microg/L was 102 +/- 12% (n = 32).     

Strong and conditional invariance principles for samples attracted to stable laws

Summary. We prove almost sure convergence of a representation of normalized partial sum processes of a sequence of i.i.d. random variables from the domain of attraction of an α-stable law, α<2. We obtain an explicit form of the limit in terms of the LePage series representation of stable laws. One consequence of these results is a conditional invariance principle having applications to option pricing as well as to resampling by signs and permutations. Received: 11 April 1994 / In revised form: 5 November 1996     

The application of a capacitive probe technique for direct observation of electromechanical processes in the guinea pig cochlea

The demonstration of evoked mechanical responses of the outer hair cells in the mammalian cochlea by indirect measurements introduces a new range of problems into direct mechanical measurements. Direct and indirect measurements indicate that the frequency spectra of evoked electromechanical responses may extend well into the range of audio frequencies, revealing a need to develop terminology and protocols for distinguishing evoked mechanical responses from the traditional traveling wave when both are apparently superimposed on the motion of the basilar membrane in the normally functioning cochlea. Details are presented of a frequency-modulation capacitive probe technique for measurement of vibrating structures of the guinea pig ear. Considerations include the design of the transducer, calibration, sensitivity, linearity, and sources of noise, as well as the influence of the technique upon the animal preparation, and in particular the issues associated with draining scala tympani for the measurement. Relative advantages and disadvantages of the technique are compared with salient features of other techniques currently available. In view of the apparent complexity of cochlear mechanics some preliminary experiments are required to elucidate some of the key questions about reverse-transduction processes in general. A "simple" first experiment is to test existence of any rectifying or motile response.     

Novel conjugated molecules from oxidation of a cyclopentadienone iron tricarbonyl complex

Oxidation of tetrakis-diethylaminocyclopentadienone iron tricarbonyl (1) electrochemically or with nitrosonium hexafluorophosphate afforded the dication of tetrakisdiethylaminocyclopentadienone (3). Oxidation of 1 with bromine and aqueous workup gave diethylaminocyclobutenedione-N,N-diethylcarboxamide (4), which was also formed by the hydrolysis of 3. The structures of the products were confirmed by X-ray crystallography.     

On bootstrap estimation of the distribution of the studentized mean

It is shown that bootstrap methods for estimating the distribution of the Studentized mean produce consistent estimators in quite general contexts, demanding not a lot more than existence of finite mean. In particular, neither the sample mean (suitably normalized) nor the Studentized mean need converge in distribution. It is unnecessary to assume that the sampling distribution is in the domain of attraction of any limit law.Now at Michigan State University