Direct laser ablation of sub-100 nm line structures into polyimide

Periodic line structures with a period of 167 nm and linewidths varying from 30 to 100 nm have been produced in polyimide by direct ablation with a KrF laser using an interferometric technique. The characteristics of this interferometer as it applies to the ablation of these line structures, including linewidth and alignment sensitivity, are analyzed. The ability to control the linewidth by varying the average incident fluence is described theoretically and demonstrated experimentally. This externally generated period of 167 nm also prevents the spontaneous growth of laser induced periodic surface structures (LIPSS).     

Pion pair production in photon-photon collisions

The process γγ→π⁺π^? was measured using the detector MD-1 at VEPP-4. The two-photon reactionse ⁺ e ^?, μ⁺ μ^? and π⁺ π^? pair production were separated using scintillation counters, Cherenkov counters and shower-range chambers. A radiation widthГ _γγ(f ₂(1270))=3.1±0.35±0.35 keV was obtained.     

An algebraic representation of the affine Bäcklund transformation

In 1980 Chern and Terng defined a Bäcklund transformation for affine minimal surfaces. In this paper we show that this Bäcklund transformation can be simply represented by an involution and translation of the affine conormal.     

The solution of orthogonal Procrustes problems for a family of orthogonally invariant norms

The orthogonal Procrustes problem involves finding an orthogonal matrix which transforms one given matrix into another in the least-squares sense, and thus it requires the minimization of the Frobenius matrix norm. We consider, the solution of this problem for a family of orthogonally invariant norms which includes the Frobenius norm as a special case.     

Influence of cultivation conditions on the production of cellulolytic enzymes withTrichoderma reesei Rutgers C30 in aqueous two-phase systems

Cellulolytic enzyme production in aqueous two-phase systems withTrichoderma reesei Rutgers C30 has been investigated. The influ ence of different phase systems, as well as addition of media compo nents and substrate on enzyme production have been studied. Extractive enzyme production in fed-batch cultivations was per formed in a phase system composed of PEG 8000 5%-Dextran T500 7% with 1% Solka-Floc BW 200 as substrate. The cellulolytic enzyme system was intermittently withdrawn with the top phase. Addition of media components every 24 h and cellulose every 72 h gave an aver age enzyme activity in the withdrawn top phase of 2.2 FPU/mL dur ing 170 h cultivation. The corresponding productivity was 18 FPU/lh. The productivity was increased to 24 FPU/l.h when media compo nents and cellulose were added every 72 h. The average enzyme con centration was then 1.6 FPU/mL. The results are discussed in relation to methods for cellulolytic enzyme production involving immobiliza tion and cell recycling.     

Biosensors: Internet Information Resources

The state of the art in the Internet information resources for biosensors is considered, and the classification of the websites is given     

Construction of equations for classical equilibrium correlation Green's functions on the basis of kinetic equations. II

Moscow Institute of Electronic Engineering. Translated from Teoreticheskaya i Matematicheskaya Fizika, Vol. 89, No. 1, pp. 132–150, October, 1991.     

Monitoring genomic alterations with a panel of oligonucleotide probes specific for various simple repeat motifs

Germline and somatic instability of the human genome was studied, using synthetic oligonucleotides specific for simple repeat motifs. The following probes were used: (GTG)5, (GACA)4, (GATA)4, (CT)8, (TTAGGG)3, (GT)8, (GAA)6 and (GGAT)4. Each of them is unique with respect to the target regions recognized in the genome. Thus compilation of the various fingerprint data provides a complex map of the genome (and its deviations). While the fingerprints of differentiated somatic tissues never showed any alterations, in tumor tissues (namely gliomas) many changes could be detected. Most of the latter reflect secondary karyological aberrations. In nearly one third of the gliomas, drastically amplified and apparently monomorphic DNA fragments were identified. This marker should make it possible to deal with causal pathogenetic mechanisms as well as novel diagnostic strategies.