Plasma B-6 vitamer and plasma and urinary 4-pyridoxic acid concentrations in young women as determined using high performance liquid chromatography.

Plasma B-6 vitamer and plasma and urinary 4-pyridoxic acid concentrations of 21 young white women, 21-27 years, having radiomonitored pyridoxal 5'-phosphate and coenzyme stimulation of erythrocyte alanine aminotransferase activities indicative of adequate vitamin B-6 status were determined in an effort to establish normal ranges for plasma B-6 vitamers. B-6 vitamers and 4-pyridoxic acid were quantitated using reversed phase high performance liquid chromatography with fluorometric and ultraviolet detection. Pyridoxal phosphate values obtained by radioenzymatic and chromatographic, fluorometric and ultraviolet, assays were highly correlated as were pyridoxine phosphate values determined using both detectors. The B-6 vitamer and 4-pyridoxic acid values of these subjects should be of use in the establishment of normal ranges of these congeners in women.     

Influence of cultivation conditions on the production of cellulolytic enzymes withTrichoderma reesei Rutgers C30 in aqueous two-phase systems

Cellulolytic enzyme production in aqueous two-phase systems withTrichoderma reesei Rutgers C30 has been investigated. The influ ence of different phase systems, as well as addition of media compo nents and substrate on enzyme production have been studied. Extractive enzyme production in fed-batch cultivations was per formed in a phase system composed of PEG 8000 5%-Dextran T500 7% with 1% Solka-Floc BW 200 as substrate. The cellulolytic enzyme system was intermittently withdrawn with the top phase. Addition of media components every 24 h and cellulose every 72 h gave an aver age enzyme activity in the withdrawn top phase of 2.2 FPU/mL dur ing 170 h cultivation. The corresponding productivity was 18 FPU/lh. The productivity was increased to 24 FPU/l.h when media compo nents and cellulose were added every 72 h. The average enzyme con centration was then 1.6 FPU/mL. The results are discussed in relation to methods for cellulolytic enzyme production involving immobiliza tion and cell recycling.     

Exact ground state of the generalized three-dimensional Shastry-Sutherland model

We generalize the Shastry-Sutherland model to three dimensions. By representing the model as a sum of the semidefinite positive projection operators, we exactly prove that the model has exact dimer ground state. Several schemes for constructing the three-dimensional Shastry-Sutherland model are proposed. Received 20 February 2002 / Received in final form 27 May 2002 Published online 17 September 2002     

Certified reference materials for the quality control of analysis in the environment

Summary The Community Bureau of Reference (BCR) has undertaken the production of a wide range of certified reference materials (CRMs) for the improvement of accuracy and measurement harmony in various areas of concern at European Community level. This paper provides an overview of the sectors of the BCR-programme in the field of environment i.e. for water systems (water, sediment, biota), soil analysis (soil, fertiliser, waste), plant analysis (aquatic and terrestrial plants), analysis related to animals (e.g. muscle or liver) or man (e.g. industry and food).     

Finite temperature QCD coupling constant —imaginary versus real time framework

The correspondence between the imaginary time and real time thermal running coupling constant is discussed. The temperature dependence is derived in pure QCD at one-loop order in a generalized momentum subtraction (MOM) renormalization scheme.     

Mössbauer study of spin-glass Fe x Zn1−x F2 system

⁵⁷Fe Mössbauer effect measurements in the diluted Ising antiferromagnet Fe _x Zn_1?x F₂ withx=0.25 andx=0.57 at temperatures between 4.2 and 55 K, are reported. DC suceptibility measurements show a spin-glass (SG) phase at low temperatures forx≤0.31. Our Mössbauer spectra show a phase transition to a SG state with antiferromagnetic order (AFSG) forx=0.25 and only antiferromagnetic order forx=0.57.     

On Nodal Sets for Dirac and Laplace Operators

We prove that the nodal set (zero set) of a solution of a generalized Dirac equation on a Riemannian manifold has codimension 2 at least. If the underlying manifold is a surface, then the nodal set is discrete. We obtain a quick proof of the fact that the nodal set of an eigenfunction for the Laplace-Beltrami operator on a Riemannian manifold consists of a smooth hypersurface and a singular set of lower dimension. We also see that the nodal set of a Δ-harmonic differential form on a closed manifold has codimension 2 at least; a fact which is not true if the manifold is not closed. Examples show that all bounds are optimal. Received: 28 October 1996 / Accepted: 3 March 1997