氟甲喹单克隆抗体制备、鉴定及间接竞争酶联免疫吸附分析法

以氟甲喹(FLU)为原料,合成4个碳原子手臂的半抗原(FLUABA),采用活泼酯法与牛血清白蛋白(BSA)偶联制备免疫抗原,通过免疫Balb/c小鼠及细胞融合,获得1株稳定分泌抗氟甲喹单克隆抗体的杂交瘤细胞株DB6-E7,其抗体亚类为IgG1,亲和力常数(KA)为8.19×108L/mol。将氟甲喹、FLUABA及6个碳原子手臂的半抗原FLUACA分别与卵清白蛋白(OVA)偶联作为包被抗原,研究异源包被对间接竞争ELISA灵敏度的影响。结果表明,异源包被可显著提高ELISA方法的灵敏度。基于最佳异源包被(FLU-OVA)的酶联免疫吸附分析法的IC50为26.33μg/L,检出限为4μg/L,定量检测范围为8.0～114μg/L(IC20～IC80)。与喹诺酮类药物及结构类似物几乎不存在交叉反应,特异性高。此方法可满足畜禽产品中氟甲喹残留的快速筛查。     

不同质量调整法在消费者价格指数(CPI)中的应用

传统的消费者价格指数(CPI)存在着一定的质量偏差,不能真实地反映实际价格的变化.直接比较法、交叠法、生产成本法、链接法等质量调整法的应用均有一定的局限性,且缺陷较多,均指插补调整借助于缺失值插补的原理,以同一组内商品价格的平均变化率作为质量调整系数,能有效地降低价格指数的质量偏差。Hedonic质量调整法是以实际资料建立的回归方程为基础,运用数学的手段科学的将质量变化程度加以量化,因此与传统的质量调整法相比含有较少的主观因素,为CPI指数的准确度量提供了科学的质量调整依据.     

Synthesis and Single-crystal Structure of a Silver(Ⅰ) Carboxyarylphosphonate: [Ag(H_2BCP)(4,4'-bipy)]·2H_2O

The title compound, a novel Ag(Ⅰ) carboxyarylphosphonate [Ag(H2BCP)(4,4'- bipy)]·2H2O (H3BCP = p-H2O3PCH2C6H4COOH, 4,4'-bipy = 4,4'-bipyridine), was synthesized by a hydrothermal reaction and characterized by elemental analysis, IR spectra and single-crystal X-ray diffraction. It belongs to monoclinic system, space group P21/c with a = 5.7038(11), b = 22.397(4), c = 5.602(4) ?, β = 106.26(3)°, V = 1913.4(7) 3, Z = 4, C18H20N2O7PAg, Mr = 515.20, Dc = 1.788 g/cm3, μ = 1.182 mm-1, F(000) = 1040, the final R = 0.0404 and wR = 0.1216 for 4178 observed reflections with I 2σ(Ⅰ). In the structure, the Ag(Ⅰ) cations are bridged by 4,4'-bipy to give rise to 1D chains running along the b axis. These chains are linked further by the interactions of O (from BCP ligands) and Ag atoms to yield 2D layers. Hydrogen bonding interactions and weak π-π stacking interactions between 4,4'-bipy rings assemble such adjacent layers to generate a 3D supramolecular architecture.     

Cu-CeO_2基阳极直接甲烷SOFC的制备及其性能

采用干压法制备了NiO-YSZ(氧化钇稳定氧化锆)/(ZrO2)0.89(Sc2O3)0.1(CeO2)0.01(10ScSZ-1CeO2)半电池,经还原-酸溶法除去NiO制备了多孔YSZ负载致密10ScSZ-1CeO2双层结构,通过浸渍法在多孔YSZ阳极基体中引入Ce、Cu的硝酸盐制备Cu-CeO2-YSZ复合阳极,结合La0.6Sr0.4Co0.2Fe0.8O3-δ(LSCF)阴极构建了Cu-CeO2-YSZ/10ScSZ-1CeO2/LSCF单元电池.通过X射线衍射(XRD)和场发射扫描电镜(FESEM)等手段对电池单元的物相、微观结构进行表征.结果表明:还原-酸溶法制备的YSZ/10ScSZ-1CeO2双层结构的YSZ基体具有孔隙率高(64%)、孔洞连通性好的微观结构,有助于采用浸渍法引入Ce、Cu硝酸盐;10ScSZ-1CeO2电解质薄膜致密无缺陷,厚约30μm.电性能测试表明所构建单元固体氧化物燃料电池(SOFC)具有良好的电性能输出,在650℃以湿H2和CH4为燃料时的最大功率密度分别为0.29和0.09W·cm-2;在700℃以湿H2和CH4为燃料时的最大功率密度分别达到0.48和0.21W·cm-2.优良的电性能主要归功于小的电解质内阻和阴极极化电阻以及良好的阳极微观结构.     

一种光栅信号细分算法的FPGA实现

提出了一种基于FPGA的光栅位移传感器信号细分处理技术,采用CORDIC(坐标旋转数字计算机)解算相位,同时结合单准确度浮点运算,将辩向、粗码计数、相位解算、粗码精码浮点运算集成于单片FPGA实现,保证了细分的准确度、细分数据的有效计算准确度及信号处理的速度、设计中采用Altera公司的Cyclone Ⅱ_EP2C20...     

掺杂对随机取向团簇粒子辐射特性的影响

利用Bruggeman有效介质理论得到了占有不同体积份额杂质的团簇粒子的等效复折射率.采用离散偶极子近似方法对包含有不同化学成分的随机取向团簇粒子的散射相函数、消光、吸收、散射效率因子、单次散射反照率以及不对称因子等辐射特性参量进行了数值计算,深入探讨了掺杂量对随机取向团簇粒子辐射特性的影响.研究表明,掺杂对随机取向团...     

Constructions of optimal difference systems of sets

Difference systems of sets (DSSs) are combinatorial configurations which were introduced in 1971 by Levenstein for the construction of codes for synchronization. In this paper, we present two kinds of constructions of difference systems of sets by using disjoint difference families and a special type of difference sets, respectively. As a consequence, new infinite classes of optimal DSSs are obtained.     

平环和的Heegaard亏格的一个下界

Let Mi, i = 1,2, be a compact orientable 3-manifold, and Ai an incompressible annulus on a component Fi of OMi. Suppose A1 is separating on F1 and A2 is non-separating on F2. Let M be the annulus sum of M1 and M2 along A1 and A2. In the present paper, we give a lower bound for the genus of the annulus sum M in the condition of the Heegaard distances of the submanifolds M1 and M2     

一种测量多相流流型的ECT图像重建算法

提出了一种新的电容层析成像图像重建算法-IMNSNOF算法.该算法在分析极小范数解的基础之上针对ECT逆问题的特点对其进行改进,并利用奇异值分解定理证明这种改进的数值稳定作用.在此基础上建立新的日标泛函,并在求解该泛函的过程中采用正则技巧对其进行改进确保数值解的稳定性.利用非闭合电极ECT传感器实测了多相流沿流向的流型...