An application of activation analysis of aluminum in biological materials

The behavior of aluminum compounds in animals was made clear by using non-destructive neutron activation analysis instead of radiotracer method. Basic aluminum sucrose sulfate (BASS) and dried aluminum hydroxide gel (DAHG) were orally administered to rats. The residual BASS and DAHG on gastric walls of rats were determined by neutron activation analysis. It was obtained the following results that BASS was found at the longer time in the stomach. For reason, it is seemed that BASS is hydrolyzed in gastric juice. And it could not find the aluminum in liver, kidney and spleen.     

Chemical studies on nitrogen heterocyclic skeleton of the Daphniphyllum alkaloids

From a biogenetic point of view, a great variety of related alkaloids isolated from the plant Daphniphyllaceae are related to one another by bond formation or fission. Thus, daphnialcohol acetate (6), a derivative of the degradation products of daphniphylline (1), was subjected to von Braun degradation followed by acid-catalyzed recyclization to give an isomer (8) of daphnialcohol, which has a new type of nitrogen heterocyclic skeleton. Furthermore, daphnilactone-B (3) was converted into a daphniphylline-type compound (19) via a plausible intermediate (21).     

Evaluation of thallium-201 myocardial emission computed tomography based on a comparison with postmortem findings

The correlative study of myocardial perfusion assessed by 201Tl myocardial ECT with the pathological finding of the heart was performed in 10 autopsied cases with mean age of 77 years old (range: 60-90 y). In 6 cases with myocardial infarction (MI) 7 perfusion defects were observed, that was, 3 in anteroseptal wall, 1 in anterolateral wall and 3 in posterior wall on the images of SPECT. Seven MIs were also found in postmortem examination. Six MIs were observed at autopsy corresponding to perfusion defect on SPECT images. In one myocardial perfusion defect at inferoposterior portion on SPECT, a non-transmural MI was found at anteroseptum. In one case with valvular disease a false positive result was obtained at posterior wall where neither myocardial necrosis nor fibrosis was observed at autopsy. This case had aortic stenosis due to bicuspid aortic valve by autopsy. The ventricle was divided into 16 segments in each of 4 short axial images to evaluate extent of MI. SPECT for extent of MI showed sensitivity of 81.9%, specificity of 96.0% and diagnostic accuracy of 92.5%. False negative segment was apt to be observed at the surrounding of non-transmural MI or basal half of left ventricle (LV) with transmural MI, while false positive segment was at posterior portion of basal half of LV. It was concluded that myocardial ECT was useful for evaluation of the site and extent of MI.     

Flow injection chemiluminescence determination of polyhydroxy phenols using luminol-ferricyanide/ferrocyanide system

It was found that the weak chemiluminescence produced from the reaction of polyhydroxy phenols with luminol in alkaline solution could be strongly enhanced by ferricyanide and ferrocyanide. Based on this found, a new flow injection chemiluminescence method is proposed for the determination of four polyhydroxy phenols: pyrogallol, phlorglucinol, quinol and resorcinol. The detection limits of the method are 0.03 μg ml⁻¹ pyrogallol, 0.03 μg ml⁻¹ phlorglucinol, 0.04 μg ml⁻¹ quinol, and 0.02 μg ml⁻¹ resorcinol. The possible mechanism of CL reactions is also discussed briefly.     

Molecular orbital calculation of Mössbauer parameters for Sn(CH3)nH4−n (n=0, 1, 2, 3) and their photoproducts in low temperature matrices

Mössbauer parameters of tin compounds, Sn(CH₃)₂H_4–n (n=0, 1, 2, 3, 4), isolated in low temperature matrices were related to electronic properties at the tin nuclei obtained by molecular orbital calculations. Structures of novel species, Sn(CH₃)₂ and Sn(CH₃)H, produced via photodissociation of matrix-isolated Sn(CH₃)₃H and Sn(CH₃)₂H₂, respectively, were determined on the basis of molecular orbital calculations as compared with Mössbauer parameters. The correlations between Mössbauer quadrupole splitting and calculated electric field gradient using STO-3G or MINI-4 were found to depend on the valence of tin atoms because of poor allowance for basis sets in describing highly polar molecules.     

Anodic stripping voltammetric determination of bismuth(III) using a Tosflex-coated mercury film electrode

A Tosflex-mercury film electrode (TMFE) was prepared by spin-coating a solution of the perfluorinated anion exchange polymer Tosflex onto a glassy carbon electrode surface followed by electrodeposition of mercury film on this electrode. This electrode was used for the determination of trace bismuth(III) which was preconcentrated onto the TMFE as anionic bismuth(III) complexes with chloride in a chloride medium. The preconcentration was carried out at a potential of-0.2 V, and the preconcentration of the bismuth(III) was enhanced significantly by the anion-exchange feature of Tosflex. The accumulated bismuth(III) was then determined by anodic square-wave stripping voltammetry (SWSV). Various parameters influencing the determination of bismuth(III) were examined in detail. With 2 min accumulation, the analytical signal versus concentration dependence was linear up to 50 ppb, and the detection limit was 0.58 ppb. This modified electrode showed good resistance to the interferences from surface-active compounds and common ions.     

Novel dye-attached macroporous films for cadmium, zinc and lead sorption: Alkali Blue 6B-attached macroporous poly(2-hydroxyethyl methacrylate)

Alkali Blue 6B-attached poly(2-hydroxyethyl methacrylate) (poly(HEMA)) microporous films were investigated as chelate forming sorbents for heavy metal removal. Poly(HEMA) microporous films were prepared by UV-initiated photo-polymerization of HEMA in the presence of an initiator (azobisisobutyronitrile (AIBN)). Alkali Blue 6B was attached covalently. These films with a swelling ratio of 58%, and carrying 14.8 mmol Alkali Blue 6B m(-2) which were then used in the removal of Cd(II), Zn(II) and Pb(II) from aqueous media. Adsorption rates were very high, equilibrium was achieved in about 30 min. The maximum adsorption of heavy metal ions onto the Alkali Blue 6B-attached films were 41.4 mmol m(-2) for Cd(II), 52.4 mmol m(-2) for Zn(II), and 64.5 mmol m(-2) for Pb(II). When the heavy metal ions competed during the adsorption from a mixture the adsorption values for Cd(II), Zn(II) and Pb(II) were quite close. Heavy metal ions were desorbed by using 0.1 M HNO(3). A significant amount of the adsorbed heavy metal ions (up to 95%) could be desorbed in 30 min. Repeated adsorption/desorption cycles showed the feasibility of these novel dye-attached microporous films for heavy metal removal.     

Purification and characterization of befunolol reductase from rabbit liver

An enzyme (befunolol reductase) which catalyzes the reduction of befunolol to dihydrobefunolol was purified from the cytosolic fraction of rabbit liver to homogeneity by various chromatographic techniques. Befunolol reductase had molecular weights of 29000 on sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and 34000 on gel filtration. The enzyme required reduced nicotinamide adenine dinucleotide phosphate (NADPH) as a cofactor and showed an optimal pH of 6.5. The apparent Km and Vmax values of the enzyme for the reduction of befunolol were 1.7 mM and 4.4 units/mg, respectively. Flavonoids, sulfhydryl reagents, heavy metals and coumarins strongly inhibited the enzyme. The enzyme catalyzed the reduction of a variety of aromatic ketones. In addition to befunolol, some ketone-containing drugs such as daunorubicin and levobunolol were efficiently reduced by the enzyme. On the basis of substrate specificities for steroids, befunolol reductase purified from the cytosolic fraction of rabbit liver appeared to be a 3 alpha-hydroxysteroid dehydrogenase.     

Anticoagulant activity of immobilized thrombomodulin

Bovine lung thrombomodulin was partially purified, and immobilized on agarose gel (Sepharose 4B). Immobilized thrombomodulin inhibited the procoagulant activity of thrombin, and enhanced the thrombin-catalyzed protein C activation. The plasma recalcification time test showed that immobilized thrombomodulin prolonged plasma clotting time. It is suggested that the immobilization of thrombomodulin will provide an antithrombogenic biomaterial able to convert thrombin from a procoagulant to an anticoagulant enzyme.     

Use of transition elements to enhance sensitivity for selenium determination by graphite-furnace atomic-absorption spectrophotometry combined with solvent extraction with the APDC-MIBK system

A microanalytical method for the measurement of selenium in waters and biological materials by a flameless atomic-absorption technique has been developed. The ammonium pyrrolidinedithiocarbamate-methyl isobutyl ketone extraction system is used for separation from interfering materials such as large amounts of alkali and alkaline earth metal salts and mineral acids. The atomic-absorption sensitivity for selenium is found to be enhanced to a large extent by co-extraction of some transition metal ions. Copper(II) has been used successfully as such an additive to diminish the volatility of selenium in the graphite furnace during the ashing step of the atomization cycle. When the aqueous phase/organic solvent volume ratio is 5 and the volume injected into the graphite furnace is 20 mul, the sensitivity for selenium is 0.3 ng/ml for 1% absorption. The relative standard deviation is ca. 2%. Interference by other metal ions is prevented by masking with EDTA. The method has been applied satisfactorily for the determination of minute amounts of selenium in waters and various biological materials.