External quality assessment in microbiology: comparison of results from Belgian and Canadian laboratories with regard to their ability to identify <Emphasis Type="Italic">Streptococcus pyogenes</Emphasis>

Clinical Microbiology Proficiency Testing (CMPT; Canada) and the Institute of Public Health (IPH; Belgium) set up a joint external quality assessment (EQA) project in which they sent identical simulated clinical samples to their respective participants. Samples were sent out as throat swabs containing Streptococcus pyogenes (as pathogen) and viridans streptococci (as commensal). Results from identification by the Belgian laboratories were excellent: 99.5% detected the group A streptococci. About 10% of the Canadian laboratories reported the absence of reportable β-haemolytic streptococci, although for most of them the issue was one of non-examination of small or pin-point streptococci. Overall, Canadian and Belgian laboratories performed well in this EQA; the joint project clearly showed that although many similarities exist, there are differences between laboratories in treating EQA samples and reporting results.     

Listening to Lanthanide Complexes: Determination of the Intrinsic Luminescence Quantum Yield by Nonradiative Relaxation

The photophysical properties of lanthanide complexes have been studied extensively; however, fundamental parameters such as the intrinsic quantum yield as well as radiative and nonradiative decay rates are difficult or even impossible to measure experimentally. Herein, a photoacoustic (PA) method is proposed to determine the intrinsic quantum yield of lanthanide complexes with lifetimes in the order of milliseconds. This method is used to determine the intrinsic quantum yields for europium(III)‐containing metallomesogens as well as terbium(III) complexes. The results show that the PA signal is sensitive to both the lifetime and the ratio of the fast‐to‐slow heat component of the samples. It is found that there is an efficient ligand sensitization and a moderate intrinsic quantum yield for the complexes. The intrinsic quantum yield of Eu³⁺ in the metallomesogens exhibits an obvious increase upon the isotropic liquid to smectic A transition. The proposed PA method is quite simple, and can contribute to a clearer understanding of the photophysical processes in luminescent lanthanide complexes.     

Choline saccharinate and choline acesulfamate: ionic liquids with low toxicities

Choline saccharinate and choline acesulfamate are two examples of hydrophilic ionic liquids, which can be prepared from easily available starting materials (choline chloride and a non-nutritive sweetener). The (eco)toxicity of these ionic liquids in aqueous solution is very low in comparison to other types of ionic liquids. A general method for the synthesis and purification of hydrophilic ionic liquids is presented. The method consists of a silver-free metathesis reaction, followed by purification of the ionic liquid by ion-exchange chromatography. The crystal structures show a marked difference in hydrogen bonding between the two ionic liquids, although the saccharinate and the acesulfamate anions show structural similarities. The optimized structures, the energetics, and the charge distribution of cation-anion pairs in the ionic liquids were studied by density functional theory (DFT) and second-order (M?ller-Plesset) perturbation theory calculations. The occupation of the non-Lewis orbitals was considered to obtain a qualitative picture of the Lewis structures. The calculated interaction energies and the dipole moments for the ion pairs in the gas phase were discussed.     

Quantification of xanthohumol, isoxanthohumol, 8-prenylnaringenin, and 6-prenylnaringenin in hop extracts and derived capsules using secondary standards

Hop is a well-known and already frequently used estrogenic phytotherapeutic, containing the interesting prenylflavonoids, xanthohumol (XN), isoxanthohumol (IXN), 8- and 6-prenylnaringenin (8-PN and 6-PN). Since the use of secondary standards can form a solution whenever the determination is required of certain components, not commercially available or too expensive, it was decided to develop an accessible HPLC-DAD method for the determination of these prenylflavonoids. The amounts were determined in hop extract and capsules, using quercetin and naringenin as secondary standards. After optimization of the sample preparation and HPLC conditions, the analysis was validated according to the ICH guidelines. The response function of XN, 8-PN, quercetin and naringenin showed a linear relationship. For the determination of XN, a calibration line of at least three concentrations of quercetin has to be constructed. The correction factors for XN (quercetin) and for 8-PN (naringenin) were validated and determined to be 0.583 for XN, and 1.296 for IXN, 8-PN and 6-PN. The intermediate precision was investigated and it could be concluded that the standard deviation of the method was equal considering time and concentration (RSD of 2.5-5%). By means of a recovery experiment, it was proven that the method is accurate (recoveries of 96.1-100.1%). Additionally, by analysing preparations containing hop extracts on the Belgian market, it was shown that the method is suitable for its use, namely the determination of XN, IXN, 8-PN and 6-PN in hop extract and capsules, using quercetin and naringenin as secondary standards.     

Metal ion detection using a fluorogenic ‘click’ reaction

A fluorogenic Cu(I)-catalyzed azide-alkyne cycloaddition reaction (CuAAC) of 3-azido-7-hydroxycoumarin has been used to detect metal ions in solution. The formation of a highly fluorescent triazole product signals the presence of Cu(I) or Cu(II) ions at micromolar concentrations. CuAAC can be modified by using an exogenous ligand like EDTA to detect and quantify Zn(II), Ca(II), and Cd(II) ions at micromolar concentrations by an allosteric mechanism. The increase in the formation of the triazole product is regulated by the release of Cu(II) from the Cu(II)-EDTA complex by the addition of a second metal ion, the allosteric effector.     

Local orientation-preserving symmetry preserving operations on polyhedra

Unifying approaches by amongst others Archimedes, Kepler, Goldberg, Caspar and Klug, Coxeter, and Conway, and extending on a previous formalization of the concept of local symmetry preserving (lsp) operations, we introduce a formal definition of local operations on plane graphs that preserve orientation-preserving symmetries, but not necessarily orientation-reversing symmetries. This operations include, e.g., the chiral Goldberg and Conway operations as well as all lsp operations. We prove the soundness of our definition as well as introduce an invariant which can be used to systematically construct all such operations. We also show sufficient conditions for an operation to preserve the connectedness of the plane graph to which it is applied.     

Block rearranging elements within matrix columns to minimize the variability of the row sums

Several problems in operations research, such as the assembly line crew scheduling problem and the k-partitioning problem can be cast as the problem of finding the intra-column rearrangement (permutation) of a matrix such that the row sums show minimum variability. A necessary condition for optimality of the rearranged matrix is that for every block containing one or more columns it must hold that its row sums are oppositely ordered to the row sums of the remaining columns. We propose the block rearrangement algorithm with variance equalization (BRAVE) as a suitable method to achieve this situation. It uses a carefully motivated heuristic—based on an idea of variance equalization—to find optimal blocks of columns and rearranges them. When applied to the number partitioning problem, we show that BRAVE outperforms the well-known greedy algorithm and the Karmarkar–Karp differencing algorithm.     

Blocking Sets in (υ,{2, 4}, 1)-Designs

The existence of blocking sets in (, {2, 4}, 1)-designs is examined. We show that for 0, 3, 5, 6, 7, 8, 9, 11 (mod 12>), blocking sets cannot exist. We prove that for each 1, 2, 4 (mod 12) there is a (, {2, 4}, 1)-design with a blocking set with three possible exceptions. The case 10 (mod 12) is still open; we consider the first four values of in this situation.     

Using equality in the Krein conditions to prove nonexistence of certain distance-regular graphs

We prove the nonexistence of a distance-regular graph with intersection array {74,54,15;1,9,60} and of distance-regular graphs with intersection arrays

{4r³+8r²+6r+1,2r(r+1)(2r+1),2r²+2r+1;1,2r(r+1),(2r+1)(2r²+2r+1)}