Multifaceted Studies of the DNA Interactions and In Vitro Cytotoxicity of Anticancer Polyaromatic Platinum(II) Complexes

This study reports a detailed biophysical analysis of the DNA binding and cytotoxicity of six platinum complexes (PCs). They are of the type [Pt(P_L)(SS‐dach)]Cl₂, where P_L is a polyaromatic ligand and SS‐dach is 1S,2S‐diaminocyclohexane. The DNA binding of these complexes was investigated using six techniques including ultraviolet and fluorescence spectroscopy, linear dichroism, synchrotron radiation circular dichroism, isothermal titration calorimetry and mass spectrometry. This portfolio of techniques has not been extensively used to study the interactions of such complexes previously; each assay provided unique insight. The in vitro cytotoxicity of these compounds was studied in ten cell lines and compared to the effects of their R,R enantiomers; activity was very high in Du145 and SJ‐G2 cells, with some submicromolar IC₅₀ values. In terms of both DNA affinity and cytotoxicity, complexes of 5,6‐dimethyl‐1,10‐phenanthroline and 2,2′‐bipyridine exhibited the greatest and least activity, respectively, suggesting that there is some correlation between DNA binding and cytotoxicity.     

Electric field-induced component dynamics in a binary liquid crystal mixture studied using two-dimensional Raman scattering

The intermolecular interactions in a mixed, binary liquid crystal system are probed using two-dimensional Raman scattering. The sample is a 50/50 by weight blend of 4-pentyl(4-cyanophenyl)cyclohexane (PCH5) and 4-methoxybenzylidene-4-butylaniline (MBBA). The response of the Raman anisotropy of the individual components to step voltages is identical. Furthermore, cross correlation analysis of oscillatory electric field experiments shows only synchronous reorientation between the two constituents. Since neat MBBA aligns perpendicular and neat CH5 aligns parallel to the applied field, the data imply strong intermolecular interactions. Additionally, as previously observed for PCH5, frequency sweep experiments indicate that the flexible parts of the molecules can reorient independently of the rigid cores.     

Bestimmung reduzierender Zucker

Ohne Zusammenfassung     

A review of “QUANTITATIVE ANALYTICAL CHEMISTRY, 4th Edition by James S. Fritz and George H. Schenk, 1979, published by Allyn and Bacon,Inc., Massachusetts,X plus 661 pages”

The ¹³C nmr for a number of malic acid esters and amide-esters have been recorded. These data confirm that alcoholysis of malic anhydride provides the α-monoester as the only isolable product.