The negative impacts on the ecosystem of antibiotic residues in the environment have become a global concern. However, little is known about the transformation mechanism of antibiotics by manganese peroxidase (MnP) from microorganisms. This work investigated the transformation characteristics, the antibacterial activity of byproducts, and the degradation mechanism of tetracycline (TC) by purified MnP from Phanerochaete chrysosporium. The results show that nitrogen-limited and high level of Mn2+ medium could obtain favorable MnP activity and inhibit the expression of lignin peroxidase by Phanerochaete chrysosporium. The purified MnP could transform 80% tetracycline in 3 h, and the threshold of reaction activator (H2O2) was about 0.045 mmol L−1. After the 3rd cyclic run, the transformation rate was almost identical at the low initial concentration of TC (77.05–88.47%), while it decreased when the initial concentration was higher (49.36–60.00%). The antimicrobial potency of the TC transformation products by MnP decreased throughout reaction time. We identified seven possible degradation products and then proposed a potential TC transformation pathway, which included demethylation, oxidation of the dimethyl amino, decarbonylation, hydroxylation, and oxidative dehydrogenation. These findings provide a novel comprehension of the role of MnP on the fate of antibiotics in nature and may develop a potential technology for tetracycline removal. 相似文献
A series of polymer chemosensors containing naphthalimide signal moiety and imide recognition moiety for the selective detection of fluoride ions (F−) were synthesized by reversible addition‐fragmentation chain transfer (RAFT) polymerization. The polymer chemosensors display obvious absorption and fluorescence variations upon the addition of F−, which can be observed by both naked eyes and optical responses. The polymers showed higher fluorescence enhancement than its monomer, and the polymers with higher molecular weight have higher sensitivity than those with lower molecular weight. Other halide anions are found to hardly induce any variation of either the absorption or fluorescence spectra.
We present a novel perspective on characterizing the spectral correspondence between nodes of the weighted graph with application to image registration. It is based on matrix perturbation analysis on the spectral graph. The contribution may be divided into three parts. Firstly, the perturbation matrix is obtained by perturbing the matrix of graph model. Secondly, an orthogonal matrix is obtained based on an optimal parameter, which can better capture correspondence features. Thirdly, the optimal matching matrix is proposed by adjusting signs of orthogonal matrix for image registration. Experiments on both synthetic images and real-world images demonstrate the effectiveness and accuracy of the proposed method. 相似文献
We investigate the effect of the polarization state of the input pulses on the visible emissions in the anomalous dispersion region of polarization-maintaining photonic crystal fiber (PM-PCF), by using ~100 fs pump pulses whose central wavelength (1064 nm) is close to the second zero dispersion wavelength (1100 nm) of the fiber, where the soliton fission mechanisms play an important role. The experimental results show that the phase-matching two-color dispersive wave emission, one at 582 nm and the other at 600 nm, is polarization-dependent and frequency shift results from the different dispersion characteristics along the two orthogonal principal axes of PM-PCF. Furthermore, it is observed for the first time that the variation of the linear input polarization angles in 45° region almost has no influence on the output spectral profiles, and the break variation of the output spectrum exists when the angle between the polarization of the linear incident pulse and the fast-axis or the slow-axis of PM-PCF is 45°, which are attributed to the coupling between the two polarization modes in high birefringent PM-PCF. 相似文献