UV IRRADIATION OF NUCLEIC ACIDS: CHARACTERIZATION OF PHOTOPRODUCTS OF THYMIDYLYL-(3'→5')-2'-DEOXY-5-FLUOROURIDINE

Abstract— The acetone-sensitized irradiation using UV-B (ultraviolet light, 280–320 nm; sunlamps) of thymidyl-yl(3'→5')deoxyfluorouridine monophosphate produces two main photoproducts. The distribution of these photo-products is dependent on the pH of the irradiation solution. At pH 6, the cis-syn cyclobutane-type photodimer is the major product, whereas at high pH (8–10) a photoadduct is the major product. These photoproducts have been identified and structurally characterized by H-1 and C-13 NMR spectroscopy. The photoadduct arises from defluorination of the 5-fluorouracil moiety. The structure of the photoadduct maintains the sugar-phosphate backbone of the starting material (d-TpF), and contains a saturated thymine moiety with an added Thy(C6-hydroxyl) and a Thy(C5)-(C5)Ura covalent bond.     

Absorption and emission of light in red emissive carbon nanodots

The structure–function relationship, especially the origin of absorption and emission of light in carbon nanodots (CNDs), has baffled scientists. The multilevel complexity arises due to the large number of by-products synthesized during the bottom-up approach. By performing systematic purification and characterization, we reveal the presence of a molecular fluorophore, quinoxalino[2,3-b]phenazine-2,3-diamine (QXPDA), in a large amount (∼80% of the total mass) in red emissive CNDs synthesized from o-phenylenediamine (OPDA), which is one of the well-known precursor molecules used for CND synthesis. The recorded NMR and mass spectra tentatively confirm the structure of QXPDA. The close resemblance of the experimental vibronic progression and the mirror symmetry of the absorption and emission spectra with the theoretically simulated spectra confirm an extended conjugated structure of QXPDA. Interestingly, QXPDA dictates the complete emission characteristics of the CNDs; in particular, it showed a striking similarity of its excitation independent emission spectra with that of the original synthesized red emissive CND solution. On the other hand, the CND like structure with a typical size of ∼4 nm was observed under a transmission electron microscope for a blue emissive species, which showed both excitation dependent and independent emission spectra. Interestingly, Raman spectroscopic data showed the similarity between QXPDA and the dot structure thus suggesting the formation of the QXPDA aggregated core structure in CNDs. We further demonstrated the parallelism in trends of absorption and emission of light from a few other red emissive CNDs, which were synthesized using different experimental conditions.

Herein we unveil the presence of a molecular fluorophore quinoxalino[2,3-b]phenazine-2,3-diamine (QXPDA) in a colossal amount in red emissive CNDs synthesized from o-phenylenediamine, a well-known precursor molecule used for CND synthesis.     

Generalized inaccuracy and a coding theorem

In this paper a coding theorem has been established by considering the inaccuracy of orderα and typeβ, and generalized average code length under the condition      

Prediction for Z-mass is a crucial test of the standard model

In schemes with oneW boson and twoZ-bosons (mediating the charged and neutral current interactions involving ordinary fermions) based on the direct product and simple groups, SU(2) × U(1) ×u′(1) andG × U(1) (G is a simple group of rank two), the following two questions are discussed. (1) What are the necessary and sufficient conditions for minimal reducibility of the effective four-fermion neutral current interaction (involving ν_μ-hadron, electron-hadron and ν_μ-electron sectors) to the corresponding prediction of the standard model? (2) In what way are the masses of the twoZ-bosons constrained relative to the mass of the neutral boson of standard model? The answers to these questions are given first by keeping the underlying Higgs structure, responsible for gauge-boson (and fermion) mass generation, completely arbitrary (called Higgs-independent case) and then by making a specific choice for the Higgs structure resulting in a natural mass relation for theW andZ-bosons that is an exact counterpart toM _W ^(S)/2 =M _Z ^(S)/2 sec² ? _W for the standard model (called Higgs-dependent case). The distinction between these two cases is brought out clearly as also that between the direct product and simple groups. Whether or not any assumption is made about the Higgs structure, with either the direct product or the simple group, it is concluded that in general there is aZ-boson lighter than the neutral boson of the standard model.     

CP nonconservation ine^ + e^ - \to t\bar tg

CP violation effects in $e^ + e^ - \to t\bar tg$ are examined. CP-odd,T _N -odd andT _N -even observables can both be used to extract information on the real and imaginary parts of Feynman amplitudes. The two Higgs doublet model with CP violating phase from neutral Higgs exchange is used to illustrate possible effects. In this particular model the expected luminosity of the future colliders seems to fall short by about one order of magnitude to detect such asymmetries.     

Covariant differential calculus on the quantum exterior vector space

We formulate a differential calculus on the quantum exterior vector space spanned by the generators of a non-anticommutative algebra satisfying