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141.
    
Matching of symmetry at interfaces is a fundamental obstacle in molecular assembly. Virus‐like particles (VLPs) are important vaccine platforms against pathogenic threats, including Covid‐19. However, symmetry mismatch can prohibit vaccine nanoassembly. We established an approach for coupling VLPs to diverse antigen symmetries. SpyCatcher003 enabled efficient VLP conjugation and extreme thermal resilience. Many people had pre‐existing antibodies to SpyTag:SpyCatcher but less to the 003 variants. We coupled the computer‐designed VLP not only to monomers (SARS‐CoV‐2) but also to cyclic dimers (Newcastle disease, Lyme disease), trimers (influenza hemagglutinins), and tetramers (influenza neuraminidases). Even an antigen with dihedral symmetry could be displayed. For the global challenge of influenza, SpyTag‐mediated display of trimer and tetramer antigens strongly induced neutralizing antibodies. SpyCatcher003 conjugation enables nanodisplay of diverse symmetries towards generation of potent vaccines.  相似文献   
142.
Abstract

A low-temperature high-pressure apparatus was designed using commercial cryogenic equipment. Pressures up to 1 GPa and temperatures down to 40 K can be obtained in a volume of up to 30 cm3. The apparatus is of the piston-cylinder type with a piston diameter of 45 mm, and the pressure can be varied at all temperatures, An adaptive temperature control system keeps the temperature inside the pressure cylinder constant to within ±0.1 K.  相似文献   
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The most detailed existing proposal for the structure of spacetime singularities originates in the work of Belinskii, Khalatnikov and Lifshitz. We show rigorously the correctness of this proposal in the case of analytic solutions of the Einstein equations coupled to a scalar field or stiff fluid. More specifically, we prove the existence of a family of spacetimes depending on the same number of free functions as the general solution which have the asymptotics suggested by the Belinskii–Khalatnikov–Lifshitz proposal near their singularities. In these spacetimes a neighbourhood of the singularity can be covered by a Gaussian coordinate system in which the singularity is simultaneous and the evolution at different spatial points decouples. Received: 17 January 2000 / Accepted: 27 November 2000  相似文献   
145.
To reduce image artifacts in diffusion tensor imaging using single shot EPI, cardiac gating may be performed to prevent brain motion induced signal void in the DWI data. In this work the necessity of performing cardiac gating with single shot echo planar imaging has been explored using F statistics of the variance in DWI data. Peripheral measurement of the cardiac cycle has been employed because of its greater convenience, and hence use, compared to ECG gating. Four volunteers have been studied. Six different trigger delays have been evaluated in the range 18-500 ms relative the peripheral pulse wave. Brain motion was found significant in and inferior to the corpus callosum area. A trigger delay of 500 ms was found to be optimal. However, a long delay allows only for one slice per R-R interval. Therefore, a minimum trigger delay may be the best choice in terms of SNR per unit of experiment time.  相似文献   
146.
 The development of a tool for calculating resonances and bound states in three-body systems described by a single-potential energy surface is reported. The method has been applied to the antiprotonic helium, doubly excited states in helium, the 11Li nuclear halo, the NeICl van der Waals molecule, and the recently found FHD reaction complex. Received November 26, 2001; accepted for publication November 28, 2001  相似文献   
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The one-particle inclusive distributions in the fragmentation regions in hadronic interactions are described in a parameter free way by a simple additative quark parton model by means of the quark fragmentation functions measured in leptoproduction. We interpret the result so that while the main interaction in hadronic collisions is due to the wee partons almost all the momenta is carried by one of the valence quarks yielding fragmentation region distributions similar to the ones in leptoproduction.  相似文献   
150.
The particle-plus-rotor model with valence particles in an i132 shell is solved exactly including single-particle splitting and pairing forces. Backbending and the spread of angular momentum of the valence shell is studied in detail for the two lowest bands and quadrupole transition rates are calculated.  相似文献   
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