A catalytic immuno-reactor for the amperometric determination of human serum albumin

A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml^?1.     

Observation of magnetic order of Cu-moment aroundx=0.12 in La2−x Ba x CuO4 and La2−x sr x CuO4 by μ+SR

Zero field positive muon spin rotation method ( ⁺SR) is applied on La_2–x Sr _x CuO₄ around forx=0.12 at which the high-T _c superconductivity (SC) is suppressed. The magnetically ordered state of Cu-moments, which is not a spin glass state but an antiferromagnetic like state, appears below 15 K for 0.105x0.120. The magnetic phase boundary is very similar to the one of the La_2–x Ba _x CuO₄ in which the structural transition from the low temperature orthorhombic (LTO) phase to the low temperature tetragonal (LTT) phase is observed aroundx=0.12. The present study suggests that there is no big difference of the electronic state of the CuO₂ plane between the La-Ba system and the La-Sr system and that the magnetic ordering of Cu-moments plays an important role for the suppression of the high-T _c SC aroundx=0.12 in both of the systems, although the LTO-LTT structural transition has not been observed yet in the La-Sr system.     

Direct and stepwise intercalation of alkylalcohols intoα-zirconium phosphate

Intercalation of alkylalcohols into -zirconium phosphate was investigated at 25°C and/or under reflux.n-Alcohols having two to five carbons and 2-propanol were taken up at 25°C. These alcohols, andn-hexanol and heptanol, also intercalated under reflux.n-Alcohols having eight to eighteen carbon atoms intercalated when a stepwise method was employed. 2-Butanol and tertiary amylalcohols intercalated under reflux using a butanol intercalate as a starting material. Ethanol-to-butanol intercalates were unstable at room temperature, losing alcohols and changing to -zirconium phosphate. The particle size of the -zirconium phosphate did not affect the intercalation of alcohols.     

Regiochemical aspects in the reaction of 2,3,5-tri-o-benzoyl-d-ribofuranosyl : Acetate with silyl enol ethers catalyzed by stannic chloride

In the reaction with silyl enol ethers catalyzed by stannic chloride, 2,3,5-tri-O-benzoyl-D-ribofuranosyl acetate behaves as an ambident electrophile; silyl enol ethers of ketones having α-hetero substituents afford C-1 adducts, whereas those of usual acyclic ketones give products arising from attack on C-2 benzoxyl group.