Specific Poly-phenolic Compounds in Cell Culture of <Emphasis Type="Italic">Vitis vinifera</Emphasis> L. cv. Gamay Fréaux

Cell cultures established from plants represent an attractive alternative to whole plants for effective production of bioactive secondary metabolites. Cell culture from Vitis vinifera L. cv. Gamay Fréaux accumulated high amounts of hydroxycinnamic acid derivatives and anthocyanins. Two new compounds were identified: 3-O-glucosylresveratrol, a stilbene derivative, abundant in cell suspension culture, and a hydroxyphenol, 4-(3,5-dihydroxyphenyl)-phenol, abundant in callus culture. The major anthocyanin monoglucosides present in cell suspension culture were cyanidin 3-O-glucoside and peonidin 3-O-glucoside, and the major cinnamoyl derivatives were cyanidin 3-O-p-coumaryl glucoside and peonidin 3-O-p-coumaryl glucoside. Three minor anthocyanin compounds were found in V. vinifera cell culture: delphinidin 3-O-glucoside, petunidin 3-O-glucoside, and delphinidin 3-O-p-coumaryl glucoside. Anthocyanin levels of cell suspension cultures increased significantly—about eight fold—after 4-day cultivation in new medium. Salicylic acid at a concentration of 50 μM did not enhance anthocyanin accumulation in cell suspension culture, and similar levels of jasmonic acid significantly reduced the anthocyanin content.     

Organometallic 3-(1H-benzimidazol-2-yl)-1H-pyrazolo[3,4-b]pyridines as potential anticancer agents

Six organometallic complexes of the general formula [M(II)Cl(η(6)-p-cymene)(L)]Cl, where M = Ru (11a, 12a, 13a) or Os (11b, 12b, 13b) and L = 3-(1H-benzimidazol-2-yl)-1H-pyrazolo[3,4-b]pyridines (L1-L3) have been synthesized. The latter are known as potential cyclin-dependent kinase (Cdk) inhibitors. All compounds have been comprehensively characterized by elemental analysis, one- and two-dimensional NMR spectroscopy, UV-vis spectroscopy, ESI mass spectrometry, and X-ray crystallography (11b and 12b). The multistep synthesis of 3-(1H-benzimidazol-2-yl)-1H-pyrazolo[3,4-b]pyridines (L1-L3), which was reported by other researchers, has been modified by us essentially (e.g., the synthesis of 5-bromo-1H-pyrazolo[3,4-b]pyridine-3-carboxylic acid (3) via 5-bromo-3-methyl-1H-pyrazolo[3,4-b]pyridine (2); the synthesis of 1-methoxymethyl-2,3-diaminobenzene (5) by avoiding the use of unstable 2,3-diaminobenzyl alcohol; and the activation of 1H-pyrazolo[3,4-b]pyridine-3-carboxylic acids (1, 3) through the use of an inexpensive coupling reagent, N,N'-carbonyldiimidazole (CDI)). Stabilization of the 7b tautomer of methoxymethyl-substituted L3 by coordination to a metal(II) center, as well as the NMR spectroscopic characterization of two tautomers 7b-L3 and 4b'-L3 in a metal-free state are described. Structure-activity relationships with regard to cytotoxicity and cell cycle effects in human cancer cells, as well as Cdk inhibitory activity, are also reported.     

π-conjugation and charge polarization in fluorene-dibenzothiophene-S,S-dioxide co-oligomers by Raman spectroscopy and quantum chemistry

The Raman spectra of a series of fluorine (F)∕dibenzothiophene-S,S-dioxide co-oligomers (S) of different length and alternation sequences in their backbones (FSF, FFSFF, FSFSF, and FASAF; A is the -C≡C- bridge) have been recorded and simulated theoretically. It is shown that Raman spectroscopy is useful to probe π conjugation and ground state electron polarization in these molecules, phenomena directly related with the existence of intramolecular charge-transfer processes owing to the combination of electron donor (fluorene) and acceptor (dibenzothiophene-S,S-dioxide) groups. Their geometric, electronic, and vibrational properties have been studied by density functional theory B3LYP∕6-311G(2d,p) quantum chemical calculations and compared with those for fluorene homo-oligomers. Comparative studies in solution∕solid∕melted phase led to the conclusion that the Raman wavenumbers are also sensitive to intermolecular interactions.     

On topological Clifford semigroups embeddable into products of cones over topological groups

In this paper we give characterizations of topological Clifford semigroups which are embeddable into Tychonoff products of topological semilattices and cones over topological groups. Also we characterize topological Clifford semigroups which embed into compact topological Clifford semigroups.     

The novel YMn2D6 deuteride synthesized under high pressure of gaseous deuterium

The novel intermetallic deuteride YMn₂D₆ was synthesized under high deuterium pressure. In order to identify the structure and characterize the magnetic properties of this deuteride the powder X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), Mn X-ray absorption near edge structure (XANES) and magnetization measurement (SQUID) were carried out. The crystal structure, the chemical state of Mn and the magnetic properties of this novel deuteride were examined and discussed. It should be noted that the structure of YMn₂D₆ (F-43m) differs dramatically from C15 symmetry of the parent material. Such a great rearrangement of the metal lattice due to deuterium absorption is rather exceptional for C15 Laves phase.     

Special points on the phase diagrams of langbeinites. The case of (x,T)-phase diagram of mixed K2xRb2(1−x)Cd2(SO4)3 crystals

We have studied special points that appear on the (x, T)-phase diagram of mixed K_2xRb_2(1?x)Cd₂(SO₄)₃ crystals. This phase diagram has been constructed issuing from the changes in domain structure occurring in the course of phase transitions. We have found that two triple points on the (x, T)-phase diagram of the above solid solutions are very close to each other in the phase space and can change their places or transform into a quadruple point. These special points are associated with disappearance of ferroelectric phases with the symmetries P1 and P2₁ taking place when Rb₂⁺ are substituted with K₂⁺ ions.     

Reaction of barbituric acid with organic azides and phosphonium ylides

Amination by organic azides has been carried out to provide aminobarbiturates by fusion of a triazole ring to the 5,6-positions of barbituric acid followed by cleavage and thermal elimination of nitrogen, whereas aza-Wittig reaction gave phosphoranylidene barbituric acid salts.      

The number of symmetric colorings of the dihedral group D3

We compute the number of symmetric r-colorings and the number of equivalence classes of symmetric r-colorings of the dihedral group D₃.     

Association of biotin with silver (I) in solution: a circular dichroism study

A strong polymeric helical complex of Ag(I) with biotin, a water-soluble vitamin also called vitamin H or B₇, has been described in the crystal phase, yet with missing spatial characteristic of its solution structure. In this work, the complexation of d-(+)-biotin with Ag⁺ in aqueous solution has been studied by means of circular dichroism spectroscopy and mass spectrometry with electrospray ionization in positive mode (MS-ESI+). For comparison, the spectra of the Nujol suspension of studied systems in the solid state were also measured. The results indicate that the Ag(I)–biotin complex has a helical arrangement of the biotin molecules in solution. Our results show that the binding process involves the thioether sulfur atom and ureido carbonyl oxygen atom, while the carboxylate side group is not coordinated with the silver. The MS-ESI experiments have revealed the composite structures of Ag⁺ with biotin of various constitutions, which represent the products of the polymeric helical Ag(I)–biotin complex.     

Verbal ordinal classification with multicriteria decision aiding

Professionals in neuropsychology usually perform diagnoses of patients’ behaviour in a verbal rather than in a numerical form. This fact generates interest in decision support systems that process verbal data. It also motivates us to develop methods for the classification of such data. In this paper, we describe ways of aiding classification of a discrete set of objects, evaluated on set of criteria that may have verbal estimations, into ordered decision classes. In some situations, there is no explicit additional information available, while in others it is possible to order the criteria lexicographically. We consider both of these cases. The proposed Dichotomic Classification (DC) method is based on the principles of Verbal Decision Analysis (VDA). Verbal Decision Analysis methods are especially helpful when verbal data, in criteria values, are to be handled. When compared to the previously developed Verbal Decision Analysis classification methods, Dichotomic Classification method performs better on the same data sets and is able to cope with larger sizes of the object sets to be classified. We present an interactive classification procedure, estimate the effectiveness and computational complexity of the new method and compare it to one of the previously developed Verbal Decision Analysis methods. The developed and studied methods are implemented in the framework of a decision support system, and the results of testing on artificial sets of data are reported.