without sharps

We show that the supremum of the lengths of prewellorderings of the reals can be , with inaccessible to reals, assuming only the consistency of an inaccessible.

     

Comparative energetics of glucose and xylose metabolism in ethanologenic recombinantEscherichia coli B

This study compared the anaerobic catabolism of glucose and xylose by a patented, recombinant ethanologenicEscherichia coli B 11303:pLOI297 in terms of overall yields of cell mass (growth), energy (ATP), and end product (ethanol). Batch cultivations were conducted with pH-controlled stirred-tank bioreactors using both a nutritionally rich, complex medium (Luria broth) and a defined salts minimal medium and growth-limiting concentrations of glucose or xylose. The value of Y_ATP was determined to be 9.28 and 8.19 g dry wt cells/mol ATP in complex and minimal media, respectively. Assuming that the nongrowth-associated energy demand is similar for glucose and xylose, the mass-based growth yield (Y _x/s , g dry wt cells/g sugar) should be proportional to the net energy yield from sugar metabolism. The value ofY _x/s was reduced, on average, by about 50% (from 0.096 g/g glu to 0.051 g/g xyl) when xylose replaced glucose as the growth-limiting carbon and energy source. It was concluded that this observation is consistent with the theoretical difference in net energy (ATP) yield associated with anaerobic catabolism of glucose and xylose when differences in the mechanisms of energy-coupled transport of each sugar are taken into account. In a defined salts medium, the net ATP yield was determined to be 2.0 and 0.92 for glucose and xylose, respectively.     

Electrospray ionization ion mobility spectrometry of carboxylate anions: ion mobilities and a mass-mobility correlation

A number of carboxylate anions spanning a mass range of 87-253 amu (pyruvate, oxalate, malonate, maleate, succinate, malate, tartarate, glutarate, adipate, phthalate, citrate, gluconate, 1,2,4-benzenetricarboxylate, and 1,2,4,5-benzenetetracarboxylate) were investigated using electrospray ionization ion mobility spectrometry. Measured ion mobilities demonstrated a high correlation between mass and mobility in both N2 and CO2 drift gases. Such a strong mass-mobility correlation among structurally dissimilar ions suggests that the carboxylate functional group that these ions have in common is the source of the correlation. Computational analysis was performed to determine the most stable conformation of the studied carboxylate anions in the gas phase under the current experimental conditions. This analysis indicated that the most stable conformations for multicarboxylate anions included intramolecular hydrogen-bonded ring structures formed between the carboxylate group and the neutral carboxyl group. The carboxylate anions that form ring confirmations generally show higher ion mobility values than those that form extended conformations. This is the first observation of intramolecular hydrogen-bonded ring conformation of carboxylate anions in the gas phase at atmospheric pressure.     

ACUTE EFFECTS OF LOW-FLUENCE ULTRAVIOLET LIGHT ON HUMAN T-LYMPHOCYTE SUBSETS

Abstract-The acute effects of low-fluence ultraviolet light, primarily between wavelengths 280 and 320 nm (ultraviolet B) on T-lymphocyte subsets were assessed in virro and in vivo by quantitative cytofluorometric analysis. In virro, 90J/m² of ultraviolet irradiation of human mononuclear cells produced a significant increase in the OKT4 (helper-inducer)/OKT8 (suppressor-cytotoxic) ratio (P>0.008), due primarily to a decrease in the OKT8 subset. In vivo, 24 h after whole-body UV irradiation of human subjects with one half of a minimal erythemal dose of ultraviolet B the OKT4/OKT8 ratios again increased (P > 0.002), again with somewhat greater effects on the OKT8 subset. These results indicate that suberythemal exposure to UV light increases OKT4/OKT8 ratios in normal human subjects because of differential effects on lymphocyte subsets. They suggest that casual exposure to sunlight mediates immunologic changes in normal human subjects.     

Effects of contact time and polarity level on adhesion between interacting surfaces

We have measured the rate at which adhesion develops between two surfaces that interact by hydrogen bonding. A poly(dimethylsiloxane) elastomer lens with a slightly oxidized surface was pushed against a polystyrene-based copolymer substrate that contained acid groups. The interaction was measured on both forming and breaking the contact using the JKR technique. The toughness of the joint, Gc, increased considerably with increasing acid content in the substrate while the apparent work of adhesion, W, measured while making the contact, decreased with increasing acid content. This decrease in W implies that the acid groups caused repulsion between the surfaces when they were not in contact, but the increase in Gc shows that they formed bonds after contact was made. The rate of increase of Gc with time was found to depend on the acid content in the substrate, but for moderate acid levels, Gc was found to saturate at values that varied approximately linearly with acid content. For 8% acid and 47% acid substrates, the rate of adhesion development over a period of 24 h could be fitted by a model assuming (i) the toughness increased linearly with areal density of bonds between the substrate and the elastomer and (ii) the rate of reaction between the substrate and the elastomer varied as the cube of the density of unreacted acid groups. This cube law may possibly be explained by the kinetics of motion of the elastomer chains on the substrate.     

Some contributions to the theory of cyclic quartic extensions of the rationals

K is a cyclic quartic extension of Q iff $\text{K = Q((rd + p d}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext><mtext>)</mtext><mtext>1</mtext><mtext>2</mtext>}}\text{)}$, where d > 1, p and r are rational integers, d squarefree, for which p² + q² = r²d for some integer q. Following a paper of A. A. Albert we show that the absolute discriminant, $\text{d(}\text{K}\text{Q}\text{)}$, of the general cyclic quartic extension is given by $\text{d(}\text{K}\text{Q}\text{) = (W}{}^2\text{d}{}^2\text{)}$ for an explicitly computable rational integer W. We next find that the relative discriminant, $\text{d(}\text{K}\text{F}\text{)}$, is given by $\text{d(}\text{K}\text{F}\text{) = (W d}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}\text{)}$, where $\text{F = Q (d}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}\text{)}$ is K′s uniquely determined quadratic subfield. We use this last result in conjunction with Corollary 3, page 359, of Narkiewicz's “Elementary and Analytic Theory of Algebraic Numbers” (PWN-Polish Scientific Publishers, 1974) to establish the following Theorem 1: If the (wide) class number of$\text{F = Q(d}{}^{\mathrm{<mtext>1</mtext><mtext>2</mtext>}}\text{)}$is odd then every cyclic quartic extensionKofQcontainingFhas a relative integral basis overF. We give a second, more organic, proof of Theorem 1 which also allows us to prove the following converse result, namely Theorem 2: Suppose the quadratic fieldFis contained in some cyclic quartic extension ofQand suppose thatFhas even (wide) class number. There then is a cyclic quartic extensionKofQcontainingFsuch thatKhas no relative integral basis overF.     

Stereochemical and mechanistic aspects of the nickel complex catalysed formation of olefins from allylic alcohols and Grignard reagents

The reaction of (+)-S-but-1-en-3-ol with phenylmagnesium bromide in the presence of bis(triphenylphosphine)nickel dichloride has been shown to proceed with inversion of configuration to yield (?)-R-3-phenylbut-1-ene.     

Photochemical Degradation of the Plant Growth Regulator 2‐(1‐Naphthyl) acetamide in Aqueous Solution Upon UV Irradiation

The photochemical degradation of 2‐(1‐naphthyl) acetamide (NAD) in aqueous solution using simulated sunlight excitation as well as UV light within the 254–300 nm range was investigated to obtain an insight into the transformation mechanism that could occur under environmental conditions. Several photoproducts were identified using HPLC/MS/MS techniques. The degradation quantum yield was found to be independent of the excitation wavelength, but showed a dependence of oxygen concentration. This increased by a factor of approximately 3 from aerated to oxygen‐free solutions. There is a clear involvement of both triplet and singlet excited states in NAD photoreactivity. The participation of singlet oxygen as a significant route in NAD degradation was ruled out by comparison with the behavior using Rose Bengal as a photosensitizer. A mechanistic pathway implying hydroxylation process through NAD radical cation species as well as an oxidation reaction by molecular oxygen is proposed. The photochemical behavior of NAD appears to mainly involve the aromatic moieties without any participation of the amide side chain. Toxicity tests clearly show that the generated primary photoproducts are responsible for a significant increase in the toxicity. However, upon prolonged irradiation this toxicity tends to decrease.