μSR investigation of single crystalline dysprosium

Muon spin rotation (μSR) data on a single crystalline sample of Dy have been obtained as function of temperature and applied hydrostatic pressure. The discontinuity observed in our new data at the phase transition at 85 K strongly suggests that the muons occupy tetrahedral interstitial sites, with a contact field B_C=-2.5 T at the lowest temperatures. Measurements with applied pressures up to 0.8 GPa show that the magnitude of the local fields increases with pressure at 0.3 GPa, and that the muon signal in the antiferromagnetic phase has essentially disappeared at 0.8 GPa. This revised version was published online in July 2006 with corrections to the Cover Date.     

Positive muon diffusion in mixed Nb‐hydrides, NbHxDy

Positive muons have been used to monitor the proton and deuteron mobilities in mixed, concentrated NbH_xD_y hydrides, with x+y= constant. The mobility of the muons decreases linearly with increasing D‐content, which is expected if the protons and the deuterons move in an uncorrelated fashion. Combined with other evidence which suggests that such correlations exist, the present observation may be interpreted as due to a destruction of a correlated motion of H’s or D’s in the vicinity of the muons. This revised version was published online in August 2006 with corrections to the Cover Date.     

Interaural fluctuations and the detection of interaural incoherence. III. Narrowband experiments and binaural models

In the first two articles of this series, reproducible noises with a fixed value of interaural coherence (0.992) were used to study the human ability to detect interaural incoherence. It was found that incoherence detection is strongly correlated with fluctuations in interaural differences, especially for narrow noise bandwidths, but it remained unclear what function of the fluctuations best agrees with detection data. In the present article, ten different binaural models were tested against detection data for 14- and 108-Hz bandwidths. These models included different types of binaural processing: independent-interaural-phase-difference/interaural-level-difference, lateral-position, and short-term cross-correlation. Several preprocessing transformations of the interaural differences were incorporated: compression of binaural cues, temporal averaging, and envelope weighting. For the 14-Hz bandwidth data, the most successful model postulated that incoherence is detected via fluctuations of interaural phase and interaural level processed by independent centers. That model correlated with detectability at r=0.87. That model proved to be more successful than short-term cross-correlation models incorporating standard physiologically-based model features (r=0.78). For the 108-Hz bandwidth data, detection performance varied much less among different waveforms, and the data were less able to distinguish between models.     

First production of ultracold neutrons with a solid deuterium source at the pulsed reactor TRIGA Mainz⋆

The production rates of ultracold neutrons (UCN) with a solid deuterium converter have been measured at the pulsed reactor TRIGA Mainz. Exposed to a thermal neutron fluence of n·cm^-2·pulse^-1, the number of detected very cold and ultracold neutrons ranges up to 200 000 at 7mol of solid deuterium (sD₂) in combination with a pre-moderator (mesitylene). About 50% of the measured neutrons can be assigned to UCN with energies E of where V _F(sD ₂) = 105 neV and V _F(guide) = 190 neV are the Fermi potentials of the sD₂ converter and our stainless steel neutron guides, respectively. Thermal cycling of solid deuterium, which was frozen out from the gas phase, considerably improved the UCN yield, in particular at higher amounts of sD₂.     

Dendronized Hyperbranched Macromolecules: Soft Matter with a Novel Type of Segmental Distribution

Dendronization of a hyperbranched polyester with different generation dendrons leads to pseudo‐dendritic structures. The hyperbranched core is modified by the divergent coupling of protected monomer units to the functional groups. Compared to dendrimers, the synthetic effort is significantly less, but the properties are very close to those of high‐generation dendrimers. The number of functional groups, molar mass, and rheology behavior even in the early generation (G1–G4) pseudo‐dendrimers strongly resembles the behavior of dendrimers in higher generations (G5–G8). Comparison of the segmental and internal structure with perfect dendrimers is performed using SANS, dynamic light scattering and viscosity analysis, microscopy and molecular dynamics simulation. The interpretation of the results reveals unique structural characteristics arising from lower segmental density of the core, which turns into a soft nano‐sphere with a smooth surface even in the first generation.     

In vitro reconstitution of α-pyrone ring formation in myxopyronin biosynthesis

Myxopyronins are α-pyrone antibiotics produced by the terrestrial bacterium Myxococcus fulvus Mx f50 and possess antibacterial activity against Gram-positive and Gram-negative pathogens. They target the bacterial RNA polymerase (RNAP) “switch region” as non-competitive inhibitors and display no cross-resistance to the established RNAP inhibitor rifampicin. Recent analysis of the myxopyronin biosynthetic pathway led to the hypothesis that this secondary metabolite is produced from two separate polyketide parts, which are condensed by the stand-alone ketosynthase MxnB. Using in vitro assays we show that MxnB catalyzes a unique condensation reaction forming the α-pyrone ring of myxopyronins from two activated acyl chains in form of their β-keto intermediates. MxnB is able to accept thioester substrates coupled to either N-acetylcysteamine (NAC) or a specific carrier protein (CP). The turnover rate of MxnB for substrates bound to CP was 12-fold higher than for NAC substrates, demonstrating the importance of protein–protein interactions in polyketide synthase (PKS) systems. The crystal structure of MxnB reveals the enzyme to be an unusual member of the ketosynthase group capable of binding and condensing two long alkyl chains bound to carrier proteins. The geometry of the two binding tunnels supports the biochemical data and allows us to propose an order of reaction, which is supported by the identification of novel myxopyronin congeners in the extract of the producer strain. Insights into the mechanism of this unique condensation reaction do not only expand our knowledge regarding the thiolase enzyme family but also opens up opportunities for PKS bioengineering to achieve directed structural modifications.     

Actual Isothermal Effects of Water‐Filtered Infrared A‐Irradiation

In this study, the athermal effects of water‐filtered infrared A (wIRA)‐irradiation (780–1400 nm) on human dermal fibroblasts were investigated. For this purpose, cells were exposed to wIRA‐irradiation (178 mW cm^?2 for 1 h), while a sophisticated experimental setup prevented warming of the samples exceeding 0.1°C. The investigated parameters were the formation of reactive oxygen species (ROS), mitochondrial membrane potential and superoxide release, protein oxidation, proliferation rate, as well as intracellular Ca²⁺‐release in single cells, most of them quantified via fluorescence microscopy and fluorimetric techniques. The existence of actual athermal wIRA‐effects is still intensively discussed, since their detection requires a careful experimental setup and both efficient and powerful temperature regulation of the exposed samples. Here, we can definitively show that some of the supposed athermal wIRA‐effects may be rather artifacts, since wIRA did not reveal any impact on the above mentioned parameters—as long as the temperature of the exposed cells was carefully maintained. Though, we were able to identify an athermal DNA‐protective wIRA‐effect, since the induced DNA damage (quantified via 8‐Oxo‐G‐formation) was significantly decreased after a subsequent UVB‐exposure. These results suggest that many of the supposed athermal wIRA‐effects can be induced by pure warming of the samples, independent from any wIRA‐irradiation.     

Project scheduling under uncertainty and resource constraints

This paper proposes a method to improve the reliability of construction schedules by optimizing schedule robustness of construction projects. The schedule robustness is measured by a criterion evaluating the impact of deviations from the initial schedule on the makespan, which arise during the realization phase of a construction project. (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)     

The discrete Babuška-Brezzi condition

Summary In the treatment of saddle point problems with finite elements a discrete Babuka-Brezzi condition is encountered. We demonstrate what this discrete Babuka-Brezzi condition entails and explain the mechanical background of this somewhat abstract inf sup condition.

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Die diskrete Babuka-Brezzi-Bedingung

Übersicht Behandelt man Sattelpunktprobleme mit Finiten Elementen, dann stößt man auf eine diskrete Babuka-Brezzi Bedingung. Wir zeigen, welche Rolle diese diskrete Babuka-Brezzi-Bedingung bei der numerischen Behandlung spielt und verdeutlichen den mechanischen Hintergrund dieser etwas abstrakten inf sup Bedingung.