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111.
F.J. Hartmann 《Hyperfine Interactions》1999,119(1-4):175-184
The experiments on metastable antiprotonic helium atoms are reviewed.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
112.
Non-contact protein microarray fabrication using a procedure based on liquid bridge formation 总被引:1,自引:1,他引:0
Michael Hartmann Johan Sjödahl Mårten Stjernström Johan Redeby Thomas Joos Johan Roeraade 《Analytical and bioanalytical chemistry》2009,393(2):591-598
Contemporary microarrayers of contact or non-contact format used in protein microarray fabrication still suffer from a number
of problems, e.g. generation of satellite spots, inhomogeneous spots, misplaced or even absent spots, and sample carryover.
In this paper, a new concept of non-contact sample deposition that reduces such problems is introduced. To show the potential
and robustness of this pressure-assisted deposition technique, different sample solutions known to cause severe problems or
to be even impossible to print with conventional microarrayers were accurately printed. The samples included 200 mg mL–1 human serum albumin, highly concentrated sticky cell adhesion proteins, pure high-salt cell-lysis buffer, pure DMSO, and
a suspension of 5-μm polystyrene beads. Additionally, a water-immiscible liquid fluorocarbon, which was shown not to affect
the functionality of the capture molecules, was employed as a lid to reduce evaporation during microarray printing. The fluorocarbon
liquid lid was shown to circumvent hydrolysis of water-sensitive activated surfaces during long-term deposition procedures. 相似文献
113.
E. Maurer F. Hartmann F. Mylius F. Foerster G. Schoene H. Schenck Alexander Fischer G. T. Dougherty M. Dittrich A. Leonhard O. L. Barnebey J. V. Mc Crae R. L. Dowdell L. Jordan J. G. Thompson J. S. Acken J. H. S. Dickenson P. Herasymenko G. Pondélik Th. R. Cunningham R. J. Price C. H. Herty G. R. Fitterer J. E. Eckel J. J. Egan W. Crafts und A. B. Kinzel 《Fresenius' Journal of Analytical Chemistry》1935,101(1-2):50-53
Ohne Zusammenfassung 相似文献
114.
115.
V. Tsurkan St. Plogmann M. Demeter D. Hartmann M. Neumann 《The European Physical Journal B - Condensed Matter and Complex Systems》2000,15(3):401-403
X-ray photoelectron spectroscopy was used to investigate ACr2S4 (, Zn, Mn, Fe, Fe:Cu) and BCr2Se4 (, Cu, Hg, Hg:Cu) single crystals. Well defined splitting of the Cr 2p core level has been found. The local magnetic moments of the Cr ions are responsible for the observed effect.
Received 2 November 1999 相似文献
116.
117.
118.
Austin Samakande Ronald D. Sanderson Patrice C. Hartmann 《Journal of polymer science. Part A, Polymer chemistry》2008,46(21):7114-7126
RAFT grafted montmorillonite (MMT) clays [i.e., N,N‐dimethyl‐N‐(4‐(((phenylcarbonothioyl)thio)methyl)benzyl)ethanammonium‐MMT (PCDBAB‐MMT) and N‐(4‐((((dodecylthio)carbonothioyl)thio)methyl)benzyl)‐N,N‐dimethylethanammo‐nium‐MMT (DCTBAB‐MMT)] of various loadings were dispersed in styrene (S) monomer and the resultant mixtures emulsified and sonicated in the presence of a hydrophobe (hexadecane) into miniemulsions. The stable miniemulsions thus obtained were polymerized to yield encapsulated polystyrene‐clay nanocomposites (PS‐CNs). The molar mass and polydispersity index (PDI) of the PS‐CNs depended on the amount of RAFT agent in the system, in accordance with the features of the RAFT process. The morphology of the PS‐CNs ranged from partially exfoliated to an intercalated morphology, depending on the percentage clay loading. The thermomechanical properties of the PS‐CNs were better than those of the neat PS polymer, and were dependent on the molar mass, PS‐CN morphology and clay loading. The similarities and differences of the PS‐CNs prepared here by miniemulsion polymerization were compared to those prepared using the same RAFT agents and polymer system by bulk polymerization (as reported by us in a previous article). © Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 7114–7126, 2008 相似文献
119.
Gottfried WAGNER Bernhard Traulich KarlM. Hartmann Dieter Oesterhelt 《Photochemistry and photobiology》1987,46(3):393-402
Quantitative action spectroscopy was performed in Halobacterium halobium. using four suited pigment mutants, namely the bacteriorhodopsin and halorhodopsin positive mutant strain M-l (BR+, HR+), the bacteriorhodopsin positive but halorhodopsin negative strain M-18 (BR+, HR-), the bacteriorhodopsin negative but halorhodopsin positive strain L-33 (BR-, HR+), and the bacteriorhodopsin and halorhodopsin negative strain L-07 (BR-, HR+). The approached questions were: First, photoenergetic synergism of halorhodopsin and bacteriorhodopsin in intact cells; second, photochromism and cellular function of the blue light-absorbing intermediates, i.e. M-412 and HR-410 in bacteriorhodopsin and in halorhodopsin, respectively. Dark-adapted cells of mutant strain M-l show wavelength-dependency of quantum yield of photo-phosphorylation, φATP. An 1.4-fold enhancement was found at 575 nm wavelength where the long wavelength absorbance bands of bacteriorhodopsin and halorhodopsin intersect. The enhancement vanished after a 30 min pulse of orange light (600 Wm-2 bandpass from 495 to 750 nm), but was restored after a 30 min pulse of blue light (100 Wm-2 bandpass from 325 to 480 nm). Photoreversibility of this enhancement probably reflects phototransformation of halorhodopsin from its ground state into its inactive intermediate, HR-410, and vice versa. The halorhodopsin-mediated enhancement with maximum quantum yield of photophosphorylation, φATP= 0.06, i.e. a quantum requirement of = 17 photons/ATP, is partly substituted by a rise in phosphate potential and explained in terms of a voltage-regulated gating effect on the H+-driven ATP-synthase, superimposed on the chemiosmotic mechanism of energy coupling. The blue-absorbing photochromic intermediate, M-412 of bacteriorhodopsin, dissipates light energy upon photoexcitation that is reflected by a spectral decline in quantum yield of photophosphorylation to a minimum value of = 0.01 at 415 nm, i.e. a quantum requirement of = 100 photons/ATP. 相似文献
120.