The current study highlights the apoptotic activity of copper oxide (CuO) nanoparticles functionalized by Glutamic acid and conjugated by thiosemicarbazone (TSC) toward human breast cancer (MCF-7) and normal (HEK293) cell lines. To this aim, the co-precipitation method was used for preparation the CuO nanoparticles. Afterward, the CuO nanoparticles functionalized by glutamic acid. After that the functionalized copper oxide nanoparticles (CuO@Glu) conjugated to thiosemicarbazone. In next step, the final nanoparticle product (CuO@Glu/TSC) was characterized by physico-chemical techniques including Fourier transform infrared (FT-IR) spectroscopy, Scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Energy dispersive X-ray analysis (EDX), zeta potential analysis and dynamic light scattering (DLS). The effects of in vitro cell viability in CuO@Glu/TSC nanoparticles showed the anti-proliferative properties with a dose-dependent manner (IC50?=?133.97 µg/ml). The IC50 of CuO@Glu/TSC on normal cell line was 230.2 µg/ml. This IC50 deference shows high cytotoxicity of CuO@Glu/TSC nanoparticles on tumor cells and low cytotoxicity on non-tumorigenic cells (HEK293) and is considered as an important aspect for this nanoparticles. Also, CuO@Glu/TSC nanoparticles had efficient effects in inhibiting the growth of breast cancer cell line (MCF-7). In addition, the CuO@Glu/TSC nanoparticle induced apoptosis symptoms which were assessed by Caspase-3 activation assay, Annexin V/ propidium iodide flow cytometry, and Hoechst 33258 staining. Further, Bax and Bcl-2 genes expression was estimated by real time PCR. The expression of Bax increased 1.69 fold, while the expression of Bcl-2 decreased 0.6 fold. The results of the current study propose that CuO@Glu/TSC nanoparticles reveal effective anti-cancer activity against breast cancer cell line.
Research on Chemical Intermediates - The preparation and characterization of an environmentally-benign and low-cost catalyst consisting of KH2PO4 supported on silica from rice husk ash is reported.... 相似文献
Journal of Thermal Analysis and Calorimetry - Porous media insert is a simple technique to enhance heat transfer, which has been used in different applications. This useful passive improvement... 相似文献
The distribution of electric field vectors was first calculated for electromembrane extraction (EME) systems in classical and cylindrical electrode geometries. The results showed that supported liquid membrane (SLM) has a general field amplifying effect due to its lower dielectric constant in comparison with aqueous donor/acceptor solutions. The calculated norms of the electric field vector showed that a DC voltage of 50 V can create huge electric field strengths up to 64 kV m−1 and 111 kV m−1 in classical and cylindrical geometries respectively. In both cases, the electric field strength reached its peak value on the inner wall of the SLM. In the case of classical geometry, the field strength was a function of the polar position of the SLM whereas the field strength in cylindrical geometry was angularly uniform. In order to investigate the effect of the electrode geometry on the performance of real EME systems, the analysis was carried out in three different geometries including classical, helical and cylindrical arrangements using naproxen and sodium diclofenac as the model analytes. Despite higher field strength and extended cross sectional area, the helical and cylindrical geometries gave lower recoveries with respect to the classical EME. The observed decline of the signal was proved to be against the relations governing migration and diffusion processes, which means that a third driving force is involved in EME. The third driving force is the interaction between the radially inhomogeneous electric field and the analyte in its neutral form. 相似文献
A magnetically separable heterogeneous nanocatalyst, Fe3O4@SiO2(CH2)3N―CH―Ar@Pd(0), was simply prepared and characterized using various techniques. The catalytic activity of this nanocatalyst was evaluated via the Suzuki–Miyaura cross‐coupling reaction of aryl halides with phenylboronic acid under various experimental conditions such as kind of base, solvent and temperature. This nanocatalyst is completely magnetically recoverable because of the superparamagnetic behaviour of Fe and can be reused with sustained selectivity and activity. 相似文献
Molecular Diversity - One of the goals of green chemistry is to use environmentally friendly solvents or remove and reduce the volume of harmful spent solvents. In this study, a novel process for... 相似文献
Research on Chemical Intermediates - In this research, synthesis of a novel magnetic 2-naphthalenesulfonic acid-grafted reduced graphene oxide quantum dot (Fe3O4-rGOQD-naphthalene-2-SO3H) via a... 相似文献
The accurate reduced potential energies for two binary gas mixtures including benzene-methanol and methane-tetrafluoromethane at low density have been obtained by direct inversion of the viscosity collision integral equations. The kinetic theory along with the extended principle of corresponding-states has been used to calculate the viscosity and dif-fusion coefficients over a wide range of temperature and composition. Good agreements between calculated and experimental data are obtained. 相似文献