Detection of Tumor Necrosis Factor Gene by Piezoelectric Nucleic Acid Senosr

A piezoelectric nucleic acid sensor was constructed ofr detection of tumor necrosis factor gene.Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal.The results show that polyethyleneimine adbesion and glutaraldehyde cross-linking method has higher sensitivity,stability and selectivity than protein A method.The solid-phase nucleic acid hybridization of oligo unclecotides and tumor necrosis factor target gene sequence were monitorde using this sensor.Tumor necrosis factor gene sequence(580bp) was detected by this nucleic acid sensor for the first time.     

Electrogenerated Chemiluminescence Based on Tris(2,2′-bipyridyl) Ruthenium(II) with Hydroxyl Carboxylic Acid

Upon the electrochemical oxidation of tris(2,2′-bipyridyl) ruthenium(II) [Ru(bpy)²⁺₃] and hydroxyl carboxylic acids, for instance, citric acid, tartaric acid, malic acid, and -gluconic acid, bright electrochemiluminescences (ECLs) were observed. Different luminescent reactions were presented depending on the applied potential. The light emission was mainly caused by the reaction between alkoxide radical ion and Ru(bpy)³⁺₃below the potential +1.80 V (vs Ag/AgCl). The luminescence intensity obviously increased because of the more complex reaction process. The luminescence wavelength of 608 nm, which could be found either at higher potential than +1.80 V or in the potential range from +1.30 to +1.80 V, confirmed that ECL was caused by Ru(bpy)²⁺₃*. The factors which affect the determination and HPLC separation of the four acids were also investigated.     

铱(Ⅲ)与2-(5-溴-2-吡啶偶氮)-5-二甲氨基苯胺的显色反应及其应用

研究了试剂2_(5_溴_2_吡啶偶氮 )_5_二甲氨基苯胺 (5_Br_PADMA)与铱 (Ⅲ )的显色反应 ;在 pH4.75～6.00的HAc -NaAc缓冲溶液中 ,铱 (Ⅲ )与5_Br_PADMA形成摩尔比为1∶2型稳定的红色络合物 ,在0.6mol/LH2SO4溶液中 ,其最大吸收波长为558nm ,表观摩尔吸光系数为5.1×104L/(mol·cm) ,铱 (Ⅲ )含量在0～0.8mg/L范围内符合比尔定律 ,采用加入适量EDTA的方法消除了Fe3 + 、Co2 + 、Ni2 + 等干扰离子 ,建立了在二元络合物体系中测定微量铱 (Ⅲ )的灵敏度高、选择性好、简便、稳定的分光光度法     

Thermal decomposition of Mn(II) complex of nicotinamide

The complex Mn(Nica)₂Cl₂ (Nica=nicotinamide) was prepared, and its decomposition was studied by means of TG and DSC. The IR spectra of the products of thermal decomposition were examined at every stage. Kinetic analysis of the first stage of thermal decomposition was performed via the TG-DTG curves, and the kinetic parameters were obtained from analysis of the TG-DTG curves with integral and differential methods. The most probable kinetic function was suggested from a comparison of the kinetic parameters. Mathematical expressions were derived for the kinetic compensation effect.

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Zusammenfassung Der Komplex Mn(Nica)₂Cl₂ (Nica steht für Nikotinamid) wurde hergestellt und seine Zersetzung mittels TG und DSC untersucht. Die thermisch zersetzten Substanzen jedes Schrittes wurden mittels IR-Spektren untersucht. Anhand der TG-DTG-Kurven erstellte man eine kinetische Analyse des ersten Schrittes der thermischen Zersetzung, die kinetischen Parameter wurden aus den TG-DTG-Kurven unter Einsatz von Integrations- und Differentialmethoden ermittelt. Durch Vergleich der kinetischen Parameter wurde die wahrscheinlichste kinetische Funktion vorgeschlagen. Mathematische Ausdrücke für den kinetischen Kompensationseffekt wurden erhalten.

     

5－（4－氯苯基）－10，15，20－三苯基卟啉及其配合物的合成、结构表征及热分解机理

合成了标题卟啉配体及其钴、镍、铜、锌和铁的五种配合物．对它们进行了元素分析、ＵＶ、ＩＲ和荧光光谱等结构表征．用ＤＴＡ、ＴＧ、ＤＳＣ等手段研究了这些新化合物的热稳定性及热分解机理，实验结果与ＨＭＯ方法计算的结果一致．     

紫外光固化聚二甲基硅氧烷脲撑氨基甲酸甲基丙烯酸酯的研究——Ⅱ亲水疏水共聚网络体系

本文以α、ω-二氨丙基封端的聚二甲基硅氧烷、甲苯二异氰酸酯、甲基两烯酸羟乙酯为原料,合成了甲基丙烯酸酯封端的聚二甲基硅氧烷聚氨酯。并以此作为反应性低聚物,以甲基丙烯酸羟乙酯为亲水性反应稀释剂,通过紫外光固化,合成了一系列聚硅氧烷含量不同的共聚网络。应用红外光谱、凝胶含量测定、热分析、动态力学性能、吸水性实验等方法,对它们的固化程度、亲水疏水性能进行了研究。结果表明:体系中存在着两相结构,增加稀释剂的含量,材料的吸水性增加。通过调节疏水性的聚二甲基硅氧烷和亲水性的甲基丙烯酸羟乙酯的含量可改变体系的亲水疏水平衡。     

水杨酸与烟草铜锌超氧化物歧化酶的相互作用

利用邻苯三酚自氧化法监测了磷酸盐缓冲体系中水杨酸（SA）对超氧化物歧化酶（SOD）活力的影响。通过荧光光谱法研究了水杨酸与超氧化物歧化酶的相互作用机制，求得两者结合的形成常数和配位数，使用Forster非辐射能量转移技术求出了两者的结合位置距色氨酸残基间的距离为2.60nm。     

Cell lysis on a microfluidic CD (compact disc)

Cell lysis was demonstrated on a microfluidic CD (Compact Disc) platform. In this purely mechanical lysis method, spherical particles (beads) in a lysis chamber microfabricated in a CD, cause disruption of mammalian (CHO-K1), bacterial (Escherichia coli), and yeast (Saccharomyces cerevisiae) cells. Interactions between beads and cells are generated in the rimming flow established inside a partially filled annular chamber in the CD rotating around a horizontal axis. To maximize bead-cell interactions in the lysis chamber, the CD was spun forward and backwards around this axis, using high acceleration for 5 to 7 min. Investigation on inter-particle forces (friction and collision) identified the following parameters; bead density, angular velocity, acceleration rate, and solid volume fraction as having the most significant contribution to cell lysis. Cell disruption efficiency was verified either through direct microscopic viewing or measurement of the DNA concentration after cell lysing. Lysis efficiency relative to a conventional lysis protocol was approximately 65%. In the long term, this work is geared towards CD based sample-to-answer nucleic acid analysis which will include cell lysis, DNA purification, DNA amplification, and DNA hybridization detection.     

The Luminescence Properties and Intramolecular Energy Transfer of Rare Earth Complexes with Aromatic Carboxylic Acids

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Oxodiperoxo molybdenum modified mesoporous MCM-41 materials for the catalytic epoxidation of cyclooctene

A hybrid heterogeneous catalyst system, which has been synthesized by covalently anchoring oxodiperoxo molybdenum chelate complexes onto the surface of mesoporous MCM-41, is highly active and truly heterogeneous for the liquid-phase epoxidation of cyclooctene with tBuOOH as the oxygen source.