化学发光技术在色谱柱后检测中的应用

化学发光与色谱联用具有选择性好、灵敏度高等优点,应用广泛.本文主要介绍近三年来化学发光包括鲁米诺、过氧草酸酯、Ru(bipy)33 、无机等体系作为液相色谱柱后检测及含氮、含硫化合物气相化学发光等体系作为气相色谱柱后检测的现状.     

BiOI负载MIL-101(Fe)@BiOI衍生BiFeO3@Fe2O3及其增强光催化性能

采用溶剂热法合成了MIL-101(Fe)@BiOI复合材料,然后将其煅烧制备光催化性能增强的BiFeO₃@Fe₂O₃@BiOI三元复合材料。采用X射线衍射、扫描电镜、紫外可见漫反射吸收光谱等测试手段表征复合材料的组成、结构、形貌。将复合材料用于可见光照射下光催化降解四环素,研究复合比例(质量比)、pH和浓度对光催化性能的影响。电化学阻抗谱、光电流响应和莫特-肖特基曲线测试分析表明三元复合材料BiFeO₃@Fe₂O₃@BiOI表现出更强的光电流响应和更低的电荷转移电阻。当BiFeO₃、Fe₂O₃和BiOI三者理论质量比为1∶1∶1时,中性条件下降解活性最高,四环素去除率为81%。     

氯过氧化物酶修饰电极对一氯二甲酮的催化氯化

通过将氯过氧化物酶溶液(Chloroperoxidase, CPO)与Nafion分散的单壁碳纳米管分散液混合后直接滴涂到玻碳电极表面制得修饰电极. 这个固定了氯过氧化物酶的碳纳米管修饰玻碳电极, 在pH=5.0的磷酸缓冲溶液中测得的循环伏安曲线上有一对准可逆的氧化还原电流峰, 经过与裸电极和没有固定氯过氧化物酶的碳纳米管修饰电极上测得的循环伏安行为对比后确认, 碳纳米管对氯过氧化物酶与电极之间的电子传递反应具有很好的促进作用. 利用该修饰电极能催化一氯二甲酮氯化为二氯二甲酮, 无需添加过氧化氢作为反应启动剂, 紫外光谱的测试结果表明, 每摩尔氯过氧化物酶可催化氯化4.0×105 mol 的一氯二甲酮, 表现出很高的催化效率.     

Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method

According to the amino acid sequence and codon preference of E. coli, the human interleukin-18（IL-18） gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.     

Isolation and Purification of Polysaccharides from Cordyceps minlitaris and Its Inhibition on the Proliferation of Rat Glomerular Mesangial Cells

The crude polysaccharide was obtained by means of the decolorization of porphyrized Cordyceps minlitaris stroma with organic solvent, extraction with hot water, precipitation in 80% ethanol, and protein removal with the Sevag method. After purification with Sephadex G-75, two of its components, CMP-1 and CMP-2, were obtained. Through the assay of gel chromatography and polarimetry, CMP-1 was identified as pure polysaccharide. The results demonstrated that CMP-1 had favorable oxidation resistance activity, which could scavenge not only oxygen-free radicals in the self-oxidation system of pyrogallic acid, but also the hydroxide-free radicals in the Fenton system. The study focused on the effects of low, medium, and high dosages of CMP-1 in rat blood serum on the proliferation of glomerular mesangial cells in vitro. Through MTT Colorimetric analysis, the activities were compared among the blank control group and the Niaoduqing positive control group CMP-1 and CMP-2. The results shows that CMP-1 was able to inhibit the proliferation of rat glomerular mesangial cells effectively. Therefore, CMP-1, one component of polysaccharides of Cordyceps minlitaris, was certainly a potential remedy for hyperplastic glomerular nephritis, whose antioxidant activity could slow down the process of chronic renal failure（CRF） to some extent.     

四川某金矿中非金属单矿物的元素分析及意义

利用中子活化分析法对四川某地区的非金属单矿物(热液脉矿物)的元素进行了测试,并利用元素地球化学研究方法对元素间的相关关系进行了分析。结果表明,在该地区可以利用元素间的伴生关系指导找矿。     

支持向量回归同时测定苯丙氨酸、酪氨酸和色氨酸的研究

在波长200~400 nm范围内,测定酪氨酸、色氨酸和苯丙氨酸混合体系的吸光度,用连续小波变换(CWT)对光谱数据进行预处理,再用支持向量回归(SVR)方法进行建模,建立了支持向量回归紫外分光光度法同时测定酪氨酸、色氨酸和苯丙氨酸的方法,用所建方法对模拟样品进行了测定。结果表明,酪氨酸、色氨酸和苯丙氨酸预测结果的回收率在98%~102%之间,测定结果准确。     

离散小波变换-支持向量回归方法同时测定苯酚、苯胺和苯甲酸

在波长范围200～400nm测定苯酚、苯胺和苯甲酸混合液的吸收光谱,用离散小波变换（DWT）对光谱数据进行处理,再用支持向量回归SVR方法进行建模,建立了离散小波变换一支持向量回归方法（DWT—SVR）。方法用于模拟样品和污染水样中苯酚、苯胺和苯甲酸的同时测定,结果满意。     

具高耐热性多芳烃结构环氧树脂的研究

综述了近年来具耐热性多芳烃结构环氧树脂的发展概况,包括萘系、蒽系和芘系环氧树脂,着重介绍了其合成途径、反应活性及结构与性能的关系.认为多芳烃结构环氧树脂具有良好的耐热耐湿性,颇具发展潜力.其中,萘系环氧树脂与蒽、芘系环氧树脂相比 ,具有较高的反应活性和耐热性,具有较高的实际应用价值.因此,近年来萘基环氧树脂受到研究人员和厂商的关注,并且一些萘系环氧树脂已经应用于生产.     

PAL-MIL铣床在操作使用中应注意的事项

介绍铣床使用时的注意事项.