Dexmedetomidine promotes the progression of hepatocellular carcinoma through hepatic stellate cell activation

Dexmedetomidine (DEX) is an anesthetic that is widely used in the clinic, and it has been reported to exhibit paradoxical effects in the progression of multiple solid tumors. In this study, we sought to explore the mechanism by which DEX regulates hepatocellular carcinoma (HCC) progression underlying liver fibrosis. We determined the effects of DEX on tumor progression in an orthotopic HCC mouse model of fibrotic liver. A coculture system and a subcutaneous xenograft model involving coimplantation of mouse hepatoma cells (H22) and primary activated hepatic stellate cells (aHSCs) were used to study the effects of DEX on HCC progression. We found that in the preclinical mouse model of liver fibrosis, DEX treatment significantly shortened median survival time and promoted tumor growth, intrahepatic metastasis and pulmonary metastasis. The DEX receptor (ADRA2A) was mainly expressed in aHSCs but was barely detected in HCC cells. DEX dramatically reinforced HCC malignant behaviors in the presence of aHSCs in both the coculture system and the coimplantation mouse model, but DEX alone exerted no significant effects on the malignancy of HCC. Mechanistically, DEX induced IL-6 secretion from aHSCs and promoted HCC progression via STAT3 activation. Our findings provide evidence that the clinical application of DEX may cause undesirable side effects in HCC patients with liver fibrosis.Subject terms: Cancer microenvironment, Cell growth     

Separation of Sulfonylurea Herbicides and Determination of Bensulfuron-methyl Residues in Tapwater by HPTLC

JPC – Journal of Planar Chromatography – Modern TLC - In this work we have studied the separation of a mixture of the sulfonylurea herbicides (metsulfuron-methyl, chlorsulfuron,...     

Two new tetranuclear complexes of the macrocyclic oxamide [MCu3] (M = Cd, Mn, macrocyclic oxamide = 1,4,8,11-tetraazacyclotradecanne-2,3-dione): syntheses, spectra and magnetic properties

Two new tetranuclear complexes of macrocyclic oxamide [Cd(CuL)₃](NO₃)₂·2.5H₂O 1, [Mn(CuL)₃(OH)₂](ClO₄)₂·Mn(H₂O)₆·4.5H₂O 2 (L = 1,4,8,11-tatraazacyclotradecanne-2,3-dione) have been synthesized, structurally characterized and preliminary investigated by magnetic studies. The structures of the title complexes consist of a tetranuclear units MCu₃ (M = Cd, Mn), the packing diagram shows two-dimensional and three-dimensional system through intermolecular weak interactions. The temperature-dependent magnetic susceptibilities of complex 2 were analyzed by an approximate treatment leading to J = −33 cm⁻¹, g_Cu = 2.10, g_Mn = 1.95 indicating antiferromagnetic exchange between Cu(II) and Mn(II) ions.     

TiO2与ZnO复合纳米结构电极的光电化学研究

利用尿素加压共沉淀法以Ti(SO4)2与Zn(NO3)2为原料制备了TiO2-ZnO复合纳米粒子, 其纳米结构电极的光电化学研究结果表明, 反应物摩尔比为3∶1, 于530 ℃煅烧制备的复合纳米结构电极的光电转换效率最高. 对吸附染料RuL2(SCN)2∶2TBA的纳米结构TiO2和各种复合纳米粒子的纳米结构电极进行光电研究的结果表明, 染料对各纳米结构电极都起到了敏化作用, 其中也是由反应物摩尔比为3∶1, 于530 ℃煅烧制备的纳米结构电极的光电转换效率最高. 对聚3-甲基噻吩修饰的纳米结构TiO2和摩尔比为3∶1, 于530 ℃煅烧的复合纳米粒子构成的纳米结构电极进行光电性能研究, 结果表明, 聚3-甲基噻吩与半导体纳米粒子之间存在p-n结, 在一定条件下p-n结的存在有利于光生电子/空穴的分离, 从而提高了光电转化效率.     

微交联聚合物降滤失剂的合成与性能

针对深井、超深井钻井液体系中的降滤失剂受长时间高温、高矿化度作用易降解的问题，以氮丙啶与3-氯丙烯反应制得-N-烯丙基氮丙啶（ALAI）； ALAI与乙二胺反应制得四烯基交联剂单体（EAAD）； EAAD与丙烯酰胺（AM）和2 丙烯酰胺基-2-甲基丙磺酸（AMPS）发生水溶液自由基共聚反应，合成了微交联共聚物降滤失剂(PAAT)，其结构和性能经¹H NMR, IR和TG-DTG表征。结果表明：PAAT可抗220 ℃高温，在高温、高矿化度条件下可维持钻井液的滤失量与流变性能，API滤失量均低于4.0 mL，高温高压滤失量均小于13.0 mL。     

Enhanced deep-red emission in donor-acceptor molecular architecture: The role of ancillary acceptor of cyanophenyl

Cyanophenyl as ancillary acceptor to modify donor-acceptor compound,plays an effective role in shifting the emission color to deep red and maintaining the luminescent efficiency.     

Application of Nafion／Cobalt Hexacyanoferrate Chemically Modified Electrodes for the Determination of Electroinactive Cations by Ion Chromatography

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多孔岩心的渗透性

The relaxation properties of water saturated in porous core are discussed and the distribution of proton transverse relaxation time and bi-exponential model of longitudiual relaxation time are obtained, which provide an illustration for two-model of fluids saturated in porous media. The cutoff value of T_1 based on relaxation specific property of fluids in porous core is adopted to improve the correlation between permeability and ?4T1b2.     

5-Br-PADCAP分光光度法测定血清锌

为建立快速、简便、灵敏的分光光度法测定血清锌 ,在表面活性剂TritonX -1 0 0存在下 ,用 2 -(5 -溴 -2 -吡啶偶氮 ) -5 -[N ,N -二羧基甲基 ]苯酚 (5 -Br-PADCAP)作显色剂 ,不去蛋白分光光度法测定血清锌。结果表明 ,锌络合物最大吸收波长为 5 5 7nm ,线性范围 0～ 5 0 0μmol L ,摩尔吸光系数为 1 1 2× 1 0 5L·mol-1·cm-1。回收率为 99 9% ,批内变异系数 (CV)为2 1 % ,批间变异系数为 2 9% ,与原子吸收分光光度法 (x)比较具有良好的相关性 ,y =1 0 1 0x-0 1 5 4,r=0 991 8,P >0 0 5。可见本法测定血清锌不必去蛋白、用血量少、方法简便、灵敏可靠 ,适合临床应用     

LaNi4.7Al0.3单颗粒微电极电化学行为的研究

报道了一种单颗粒微电极制备新方法, 并研究了LaNi4.7Al0.3球形单颗粒微电极的电化学行为.