RNA detection using peptide-inserted <Emphasis Type="Italic">Renilla</Emphasis> luciferase

A novel complementation system with short peptide-inserted-Renilla luciferase (PI-Rluc) and split-RNA probes was constructed for noninvasive RNA detection. The RNA binding peptides HIV-1 Rev and BIV Tat were used as inserted peptides. They display induced fit conformational changes upon binding to specific RNAs and trigger complementation or discomplementation of Rluc. Split-RNA probes were designed to reform the peptide binding site upon hybridization with arbitrarily selected target RNA. This set of recombinant protein and split-RNA probes enabled a high degree of sensitivity in RNA detection. In this study, we show that the Rluc system is comparable to Fluc, but that its detection limit for arbitrarily selected RNA (at least 100 pM) exceeds that of Fluc by approximately two orders of magnitude.     

Self-Gravitating Klein–Gordon Fields in Asymptotically Anti-de-Sitter Spacetimes

We initiate the study of the spherically symmetric Einstein–Klein–Gordon system in the presence of a negative cosmological constant, a model appearing frequently in the context of high-energy physics. Due to the lack of global hyperbolicity of the solutions, the natural formulation of dynamics is that of an initial boundary value problem, with boundary conditions imposed at null infinity. We prove a local well-posedness statement for this system, with the time of existence of the solutions depending only on an invariant H ²-type norm measuring the size of the Klein–Gordon field on the initial data. The proof requires the introduction of a renormalized system of equations and relies crucially on r-weighted estimates for the wave equation on asymptotically AdS spacetimes. The results provide the basis for our companion paper establishing the global asymptotic stability of Schwarzschild-Anti-de-Sitter within this system.     

Dynamics of a running gear with IRWs on curved tracks for a robust control development

As a major task of the DLR-internal project “Next Generation Train”, robust state feedback control with gain scheduling was sucessfully applied to guide the experimental running gear with independently rotating wheels (IRWs) at a 1:5 scaled roller rig, see [1]. However, the adaptation of the control structure to the 1:1 multibody model requires to additionally consider the properties of curved tracks. For that reason, an analytical model of a running gear with IRWs is deduced using Euler-Lagrange-equations and taking superelevation and track curvature into account. Furthermore, the complexity of the system is reduced to allow for a robust feedback control synthesis and feed-forward control including model inversion. Finally, the model is discussed and a first approach for a feed-forward control in transition curves is shown. (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)