Importance of compositional homogeneity of macromolecular chains for UCST‐type transitions in water: Controlled versus conventional radical polymerization

The change of polymerization method from conventional free radical polymerization to the reversible addition fragmentation chain transfer (RAFT) method provided thermoresponsive behavior of upper critical solution temperature (UCST)‐type in water to copolymers of styrene (St) and acrylamide (AAm). Sample preparation conditions (temperature and time of dissolution) for turbidity measurements could also significantly influence the thermoresponsive behavior of polymers based on AAm. Poly(AAm‐co‐St)s made by RAFT method till high conversions showed sharp cloud points ranging 50–62 °C with low hysteresis in water depending upon the copolymer composition. Samples for turbidity measurements were prepared under optimized conditions, that is, 70 °C for 1.5 h. In contrast, the copolymers made by conventional radical polymerization in all copolymer composition range were not thermoresponsive. The example [poly(AAm‐co‐St)] emphasizes the importance of compositional homogeneity of macromolecular chains for showing UCST‐type transitions in water for a system with wide difference in reactivity ratios of the comonomers. Since, examples of polymeric systems showing UCST in water are not too many, this work highlights how compositional homogeneity would help in developing many more systems with tuned cloud points. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014 , 52, 1878–1884     

Synthesis of de novo designed small-molecule inhibitors of bacterial RNA polymerase

The de novo molecular design program SPROUT has been used in conjunction with the X-ray crystal structure of RNA polymerase (RNAP) from Thermus aquaticus to produce a novel enzyme inhibitor scaffold. A short and efficient synthesis of molecules corresponding to this scaffold has been developed and in keeping with the design predictions, the resulting inhibitors displayed useful levels of inhibition of Escherichia coli RNAP.     

Mechanism design for a multicommodity flow game in service network alliances

We study a collaborative multicommodity flow game where individual players own capacity on the edges of the network and share this capacity to deliver commodities. We present membership mechanisms, by adopting a rationality based approach using notions from game theory and inverse optimization, to allocate benefits among the players in such a game.     

Optimal Existence Conditions for the Periodic Delay ϕ-Laplace Equation with upper and lower Solutions in the Reverse order

In this paper, we show that the monotone iterative technique produces two monotone sequences that converge uniformly to extremal solutions for the periodic delay ?-Laplace equation. Moreover, we obtain optimal existence conditions with upper and lower solutions in the reverse order.     

Quasi-planar graphs have a linear number of edges

A graph is calledquasi-planar if it can be drawn in the plane so that no three of its edges are pairwise crossing. It is shown that the maximum number of edges of a quasi-planar graph withn vertices isO(n).Work on this paper by Pankaj K. Agarwal, Boris Aronov and Micha Sharir has been supported by a grant from the U.S.-Israeli Binational Science Foundation. Work on this paper by Pankaj K. Agarwal has also been supported by NSF Grant CCR-93-01259, by an Army Research Office MURI grant DAAH04-96-1-0013, by an NYI award, and by matching funds from Xerox Corporation. Work on this paper by Boris Aronov has also been supported by NSF Grant CCR-92-11541 and by a Sloan Research Fellowship. Work on this paper by János Pach, Richard Pollack, and Micha Sharir has been supported by NSF Grants CCR-91-22103 and CCR-94-24398. Work by János Pach was also supported by Grant OTKA-4269 and by a CUNY Research Award. Work by Richard Pollack was also supported by NSF Grants CCR-94-02640 and DMS-94-00293. Work by Micha Sharir was also supported by NSF Grant CCR-93-11127, by a Max-Planck Research Award, and by grants from the Israel Science Fund administered by the Israeli Academy of Sciences, and the G.I.F., the German-Israeli Foundation for Scientific Research and Development. Part of the work on this paper was done during the participation of the first four authors in the Special Semester on Computational and Combinatorial Geometry organized by the Mathematical Research Institute of Tel Aviv University, Spring 1995.     

Label placement by maximum independent set in rectangles

Motivated by the problem of labeling maps, we investigate the problem of computing a large non-intersecting subset in a set of n rectangles in the plane. Our results are as follows. In O(n log n) time, we can find an O(log n)-factor approximation of the maximum subset in a set of n arbitrary axis-parallel rectangles in the plane. If all rectangles have unit height, we can find a 2-approximation in O(n log n) time. Extending this result, we obtain a (1 + 1/k)-approximation in time O(n log n + n^2k−1) time, for any integer k ≥ 1.     

Amperometric immunosensor for detection of antibodies of Salmonella typhi in patient serum

An amperometric sensor for detection of antibodies to Salmonella typhi in the serum of patients was developed. This involved usage of screen-printed electrodes and recombinant flagellin fusion protein. An indirect enzyme-linked immunosorbant assay was used for detection of antibodies to S. typhi in the patient serum. The screen-printed electrodes were made using polystyrene and graphite. These electrodes were tested for their ability to detect 1-naphthol, which is the product formed due to the hydrolysis of the substrate 1-naphthyl phosphate by the enzyme alkaline phosphatase. These electrodes were coated with recombinant flagellin fusion protein made by recombinant DNA technology and blocked with bovine serum albumin (BSA). Further they were incubated with patient serum and goat anti-human alkaline phosphatase conjugate. The immunosensing was performed by using amperometric method. Pooled human serum samples from apparently healthy individuals were used as control. Both the pooled healthy human serum samples and patient sera were subjected to Widal agglutination test and amperometric method. A 100% correlation was found between the Widal test and amperometric method. The time taken for the detection by electrochemical method is 1 h and 15 min, while the time taken by Widal test is 18 h.     

Singular positone and semipositone boundary value problems of second order delay differential equations

In this paper we present some new existence results for singular positone and semipositone boundary value problems of second order delay differential equations. Throughout our nonlinearity may be singular in its dependent variable.     

An infinite interval problem arising in circularly symmetric deformations of shallow membrane caps

Existence results are presented for singular boundary value problems modelling phenomena which arise in the theory of shallow membrane caps.     

[6+3] Cycloaddition of fulvenes with 3-oxidopyrylium betaine: a novel methodology for the synthesis of fused cyclooctanoid natural products

Fulvenes undergo an easy [6+3] cycloaddition with 3-oxidopyrylium betaines leading to 5-8 fused oxabridged cyclooctanoids.