Insertion mechanism of a poly(ethylene oxide)-poly(butylene oxide) block copolymer into a DPPC monolayer

Interactions between amphiphilic block copolymers and lipids are of medical interest for applications such as drug delivery and the restoration of damaged cell membranes. A series of monodisperse poly(ethylene oxide)-poly(butylene oxide) (EOBO) block copolymers were obtained with two ratios of hydrophilic/hydrophobic block lengths. We have explored the surface activity of EOBO at a clean interface and under 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers as a simple cell membrane model. At the same subphase concentration, EOBO achieved higher equilibrium surface pressures under DPPC compared to a bare interface, and the surface activity was improved with longer poly(butylene oxide) blocks. Further investigation of the DPPC/EOBO monolayers showed that combined films exhibited similar surface rheology compared to pure DPPC at the same surface pressures. DPPC/EOBO phase separation was observed in fluorescently doped monolayers, and within the liquid-expanded liquid-condensed coexistence region for DPPC, EOBO did not drastically alter the liquid-condensed domain shapes. Grazing incidence X-ray diffraction (GIXD) and X-ray reflectivity (XRR) quantitatively confirmed that the lattice spacings and tilt of DPPC in lipid-rich regions of the monolayer were nearly equivalent to those of a pure DPPC monolayer at the same surface pressures.     

Low temperature organ preservation, blood vessels and the Human Tissue Act in 2007: impact and implications

The impact of the enactment of the Human Tissue Act is discussed in relation to procedures currently applied during cadaveric organ procurement for transplantation within the UK. The major effect has been to regulate the storage of blood vessels for use as accessory grafts during transplantation, where hypothermic preservation beyond 2 days has historically been practiced and now falls within the remit of the Act concerning storage of human tissues. The benefits and drawbacks of considering cryopreserved blood vessels in such situations are also debated.     

Flavor evolution of the neutronization neutrino burst from an O-Ne-Mg core-collapse supernova

We present results of 3-neutrino flavor evolution simulations for the neutronization burst from an O-Ne-Mg core-collapse supernova. We find that nonlinear neutrino self-coupling engineers a single spectral feature of stepwise conversion in the inverted neutrino mass hierarchy case and in the normal mass hierarchy case, a superposition of two such features corresponding to the vacuum neutrino mass-squared differences associated with solar and atmospheric neutrino oscillations. These neutrino spectral features offer a unique potential probe of the conditions in the supernova environment and may allow us to distinguish between O-Ne-Mg and Fe core-collapse supernovae.