Linear equations depending differentiably on a parameter

We investigate operator functionsT(x) inBanach spaces, depending differentiably (meaning of classC orC ) on a parameterx and enjoying a certain regularity property. Iff is a given differentiable function such that the equationT(x)e=f(x) is solvable for eachx then the existence of a functione is proved which belongs to the same differentiability class asf andT, solving the equationT(x)e(x)f(x) identically inx. As an application we extend a result ofJ. Leiterer [9] and give a comprehensive answer to a question posed byJ.L. Taylor in [15] concerning the exactness of certain cochain complexes.     

Planning systems and artificial intelligence

Traditional O.R. systems are compared with problem solving in Artificial Intelligence via Expert Systems. The discussion centers on explicit knowledge representation. The general aspects are illustrated by two planning systems:

1. LESP 2: A learning system for inspection plan generation
2. IDA: A system for finding functions and solutions in construction

     

Nanoscale organization of the pathogen receptor DC-SIGN mapped by single-molecule high-resolution fluorescence microscopy.

DC-SIGN, a C-type lectin exclusively expressed on dendritic cells (DCs), plays an important role in pathogen recognition by binding with high affinity to a large variety of microorganisms. Recent experimental evidence points to a direct relation between the function of DC-SIGN as a viral receptor and its spatial arrangement on the plasma membrane. We have investigated the nanoscale organization of fluorescently labeled DC-SIGN on intact isolated DCs by means of near-field scanning optical microscopy (NSOM) combined with single-molecule detection. Fluorescence spots of different intensity and size have been directly visualized by optical means with a spatial resolution of less than 100 nm. Intensity- and size-distribution histograms of the DC-SIGN fluorescent spots confirm that approximately 80 % of the receptors are organized in nanosized domains randomly distributed on the cell membrane. Intensity-size correlation analysis revealed remarkable heterogeneity in the molecular packing density of the domains. Furthermore, we have mapped the intermolecular organization within a dense cluster by means of sequential NSOM imaging combined with discrete single-molecule photobleaching. In this way we have determined the spatial coordinates of 13 different individual dyes, with a localization accuracy of 6 nm. Our experimental observations are all consistent with an arrangement of DC-SIGN designed to maximize its chances of binding to a wide range of microorganisms. Our data also illustrate the potential of NSOM as an ultrasensitive, high-resolution technique to probe nanometer-scale organization of molecules on the cell membrane.     

Thermodynamic properties of transition metals in aqueous solution. 2. The enthalpies of mixing of aqueous solutions of CoCl2, CuCl2, and MnCl2 with NaCl at varying ionic strength at 25°C

Enthalpies of mixing (_m H) aqueous solutions of CoCl₂, CuCl₂, and MnCl₂ with NaCl solutions were measured at constant ionic strengths of 0.5, 1.0, and 3.0 molal at 25°C. The excess enthalpy equations of Pitzer were then fit to the resulting _m H data. The resulting parameters are the temperature derivatives of the activity coefficient mixing parameters in the Pitzer system. The heat of mixing data for CoCl₂ and CuCl₂ were in agreement with earlier isomolal results by other workers.