C19-二萜生物碱贡乌生的结构研究

毛茛科植物贡嘎乌头 ( Aconitum liljestrandii)具有镇痛、镇静、祛风湿等功效 ,产于我国西藏东部及四川西部 [1] .我们从其块根中分得 1 8个单体 ,其中 1 6个为已知生物碱 [2 ,3] ,2个为新生物碱 [3] .本文报道新的 C19-二萜生物碱贡乌生 ( Liljestrandisine) 1的结构 .1　实验部分1 .1　仪器与试剂　 Boetius微量熔点测定仪 ;Nicolet FTIR2 0 0 SXV型红外光谱仪 ,KBr压片 ;BrukerAC- E2 0 0和 Varian Unity INOVA40 0 /5 4核磁共振仪 ,溶剂为 CDCl3,TMS为内标 ;VG Autospec30 0 0型和 VG70 A型质谱仪 ;Pekin Polarimete…     

碳纳米管电极超大容量离子电容器交流阻抗特性

采用碳纳米管作为超大容量离子电容器的电极材料,应用交流阻抗频谱法,研究了超大容量离子电容器的频率响应特性.结果表明,用碳纳米管块作电极,超大容量离子电容器在频率250 mHz以下出现“电荷饱和”；而用活性炭块作电极, 超大容量离子电容器在频率为100 mHz时仍未出现“电荷饱和”,这说明碳纳米管电极超大容量离子电容器的频率响应特性优于活性炭电极超大容量离子电容器的频率响应特性.上述两类超大容量离子电容器的阻抗谱中均出现倾角约为45°的直线段,其相位角均远小于理想电容器的相位角90°.     

Norditerpenoid Alkaloids from the Roots of Aconitum hemsleyanum Pritz.var.Pengzhouense

In continuation of our studies on Aconitum hemsleyanum Pritz.var. pengzhouense, two new norditerpenoid alkaloids, pengshenines A(1) and B(2),have isolated from the roots of the plants and their strucures were elucidated by 1D-and 2D-NMR.     

阿斯美胶囊中四种组分的高效液相色谱分析

建立了反相高效液相色谱法测定阿斯美胶囊中氨茶碱、盐酸甲氧非那明、马来酸氯苯那敏及那可汀四种组分的含量。色谱柱为ＳｐｈｅｒｉｓｏｒｂＣ８，５μｍ，２００ｃｍ×４０ｍｍＩＤ，流动相为体积分数０５％的三乙胺溶液（磷酸调节ｐＨ５５）＋乙腈＋甲醇（５５０＋３６８＋８２），检测波长为２６４ｎｍ，线性范围分别为：氨茶碱３１２５～２５０ｍｇ／Ｌ，γ＝０９９９６，盐酸甲氧非那明１５６２５～１２５０ｍｇ／Ｌ，γ＝１００００，马来酸氯苯那敏２５～２００ｍｇ／Ｌ，γ＝０９９９９，那可汀８７５～７００ｍｇ／Ｌ，γ＝０９９９９；回收率（ｎ＝３）分别为１０１２％，１００８％，９９９％，９９５％；精密度：日内平均ＲＳＤ（ｎ＝６）分别为０７％，０４％，０４％，０６％；日间平均ＲＳＤ（ｎ＝３）分别为１０％，１０％，１１％，１２％。     

蜜蜂外激素9－ODA和9－HDA的合成

６－溴－２－己酮在Ｌｉ２ｃｕＣｌ４催化下与γ－溴代巴豆酸甲酯进行格氏偶联，合成了蜂王物质９－羰基－（Ｅ）－２－癸烯酸（９－ＯＤＡ），将其还原得到另一蜂王物质９－羟基－（Ｅ）－２－癸烯酸（９－ＨＤＡ）。该法是合成９－ＯＤＡ和９－ＨＤＡ的新路线。     

One-step hexaplex immunoassays by on-line paper substrate-based electrospray ionization mass spectrometry for combined cancer biomarker screening

Mass spectrometry (MS) is attractive as a multiplexed immunoassay readout benefiting from its high sensitivity, speed and mass resolution. Here, a simple paper-based hexaplex immunoassay with an on-line MS readout was proposed, using functionalized paper as the immune substrates, along with rhodamine-based mass tags assembled on gold nanoparticles prepared as the mass probes (MPs). Simultaneous immune capture and labeling were conducted in one step on paper substrates in 96-well plates with a high throughput within 30 minutes, and the on-line efficient dissociation of the mass tags highly facilitated the hexaplex readout of the immune signals by a newly established on-line paper substrate-based electrospray ionization-MS setup. Six MPs were synthesized for the simultaneous quantification of six important cancer protein markers (cancer antigen 15-3, cancer antigen 19-9, carcinoma embryonic antigen, cancer antigen 125, human epididymis protein 4, and alpha fetoprotein) using only 10 μL serum, presenting satisfactory sensitivity, accuracy and specificity. This platform was further tested in screening for the six biomarkers in serum samples of patients with breast, liver and gastric cancers, showing its high potential for sensitive and specific early cancer diagnosis.

On-line paper substrate based electrospray ionization mass spectrometry for hexaplex immunoassays.     

Antidepressant-like effect of triterpenoids extracts from Poria cocos on the CUMS rats by 16S rRNA gene sequencing and LC–MS metabolomics

Abstract

Poria cocos is an edible medicinal mushroom with the effects of inducing diuresis, excreting dampness, invigorating the spleen and tranquilizing the mind. The triterpenoids of Poria cocos as the main active ingredients have shown health benefits of the central nervous system of the human body. Accordingly, this study aimed at further understanding the antidepressant-like effect and a potential mechanism of the triterpenoids extracts from Poria cocos (TPC). As a result, the TPC significantly ameliorated depression-like behaviors on chronic unpredictable mild stress (CUMS) rats, and restored the levels of brain-derived neurotrophic factor and nerve growth factor in the hippocampus of rats. Gut microiota analysis revealed that TPC could increase the biodiversity and markedly regulate the relative abundance of [Prevotella], Allobaculum and Ochrobactrum of CUMS rats. The cecal contents metabolomics pointed to thirteen biomarkers associated with TPC antidepressant effect, which involved in primary bile acid biosynthesis, taurine and hypotaurine metabolism, arginine and proline metabolism. Correlation analysis further showed that there was a strong correlation relationship between the gut microbiota and the cecal contents metabolites, especially compounds involved in energy metabolism, inflammation and immunity. In conclusion, TPC showed a potential antidepressant effect, which was possibly mediated the gut microbiota and cecal contents metabolism.     

两种含锌化合物对慢性染铅大鼠海马NADPH-d阳性神经元影响的比较

为探讨硫酸锌(ZnSO4)和牛磺酸锌(TZC)拮抗铅对学习记忆的损害的不同，采用NADPH-黄递酶(NADPH-d)组化，观察了饮用含0.2g／L醋酸铅(PbAc)饮水和含不同剂量(5．0，15．0g／kg)ZnSO4、(5．9、17．7g／kg)TZC饲料喂养的大鼠海马一氧化氮合酶(NOS)活性的变化。结果表明，各补锌组均不同程度地缓解铅致NOS活力减低，铅 低TZC组、铅 高ZnSO4组和铅 低ZnSO4组大鼠海马神经元的NOS活性明显高于染铅组，其中铅 低TZC组的保护作用最显著，而铅 高TZC组的NOS活性明显低于对照组，而接近染铅组水平。结论是锌对抗铅的毒性作用与锌的化学结合形式、锌的剂量密切相关。     

邻羟基萘基荧光酮－DDMBA光度法测定微量锗

邻羟基萘基荧光酮－ＤＤＭＢＡ光度法测定微量锗冯永兰，邝代治（衡阳师范专科学校化学系衡阳，４２１００８）关键词：分光光度法，锗，邻羟基萘基荧光酮，溴化十二烷基二甲基苄铵苯基荧光酮类试剂应用于锗的测定已有报导￣［１－６］。但邻羟基萘基荧光酮（β－ＨＮＦ）...     

A visible-light activated [2 + 2] cycloaddition reaction enables pinpointing carbon–carbon double bonds in lipids

The precise location of C C bonds in bioactive molecules is critical for a deep understanding of the relationship between their structures and biological roles. However, the traditional ultraviolet light-based approaches exhibited great limitations. Here, we discovered a new type of visible-light activated [2 + 2] cycloaddition of carbonyl with C C bonds. We found that carbonyl in anthraquinone showed great reactivities towards C C bonds in lipids to form oxetanes under the irradiation of visible-light. Combined with tandem mass spectrometry, this site-specific dissociation of oxetane enabled precisely locating the C C bonds in various kinds of monounsaturated and polyunsaturated lipids. The proof-of-concept applicability of this new type of [2 + 2] photocycloaddition was validated in the global identification of unsaturated lipids in a complex human serum sample. 86 monounsaturated and polyunsaturated lipids were identified with definitive positions of C C bonds, including phospholipids and fatty acids even with up to 6 C C bonds. This study provides new insights into both the photocycloaddition reactions and the structural lipidomics.

A new visible-light activated [2 + 2] cycloaddition reaction was discovered and enabled pinpointing carbon–carbon double bonds in lipids.