Sea Anemone Heteractis crispa Actinoporin Demonstrates In Vitro Anticancer Activities and Prevents HT-29 Colorectal Cancer Cell Migration

Actinoporins are the most abundant group of sea anemone cytolytic toxins. Their membranolytic activity is of high interest for the development of novel anticancer drugs. However, to date the activity of actinoporins in malignant cells has been poorly studied. Here, we report on recombinant analog of Hct-S3 (rHct-S3), belonging to the combinatory library of Heteractis crispa actinoporins. rHct-S3 exhibited cytotoxic activity against breast MDA-MB-231 (IC₅₀ = 7.3 µM), colorectal HT-29 (IC₅₀ = 6.8 µM), and melanoma SK-MEL-28 (IC₅₀ = 8.3 µM) cancer cells. The actinoporin effectively prevented epidermal growth factor -induced neoplastic transformation of JB6 Cl41 cells by 34% ± 0.2 and decreased colony formation of HT-29 cells by 47% ± 0.9, MDA-MB-231 cells by 37% ± 1.2, and SK-MEL-28 cells by 34% ± 3.6. Moreover, rHct-S3 decreased proliferation and suppressed migration of colorectal carcinoma cells by 31% ± 5.0 and 99% ± 6.4, respectively. The potent anti-migratory activity was proposed to mediate by decreased matrix metalloproteinases-2 and -9 expression. In addition, rHct-S3 induced programmed cell death by cleavage of caspase-3 and poly (ADP-ribose) polymerase, as well as regulation of Bax and Bcl-2. Our results indicate rHct-S3 to be a promising anticancer drug with a high anti-migratory potential.     

Modification of polyvinyl chloride with Na(K) salts of 1,2,4-triazole and 1,2,3-benzotriazole

Russian Journal of Applied Chemistry - The possibility of polyvinyl chloride modification by nucleophilic substitution of chlorine with sodium and potassium salts of 1,2,4-triazole and...     

Electrosurface Properties of Hydr(oxides) and Oxide Nanostructures in 1 : 1 Electrolyte Solutions: 2. Electrokinetic Characteristics of Boehmite, Goethite, and Silicon Dioxide

The electrophoretic mobility of particles of silicon dioxide, aluminum hydroxide, and iron hydroxide was measured as a function of pH in NaCl and KCl background solutions. Isoelectric points for the investigated objects were determined. Electrokinetic potentials were calculated with allowances for the particle shape and the polarization of the electrical double layer within the framework of the Overbeek–Boes–Wiersema model.     

Nanocomposites with silver nanoparticles based on copolymer of 1-vinyl-1,2,4-triazole with N-vinylpyrrolidone

Russian Chemical Bulletin - New functional silver polymer nanocomposites were synthesized using copolymer of 1-vinyl-1,2,4-triazole with N-vinylpyrrolidone as a stabilizer of the formed metal...     

Phase formation in the systems Rb2MoO4-BaMoO4-R2(MoO4)3, R = Nd,Sm, Eu,Gd

Phase formation in the subsolidus area of the systems Rb₂MoO₄-BaMoO₄-R₂(MoO₄)₃ was studied by the methods of X-ray and differential-thermal analysis. In these systems ternary molybdates RbBaR(MoO₄)₃ crystallizing in the monoclinic crystal system (space group P2_1/n ) are formed. Their crystallographic parameters were calculated, and temperature dependences of the electrical conduction, dielectric permeability, and dielectric loss tangent were studied.     

Effect of Heat Treatment on the Formation of Copper Active Centers Obtained by the Interaction of Copper Chloride with H-Mordenite

Physics of the Solid State - The effect of temperature conditions on the formation of copper active sites obtained by solid state ion exchange between copper chloride and H-mordenite zeolite using...     

Structure and Electrosurface Properties of Porous Glasses with Different Compositions in KCl and NaCl Solutions

For porous glasses with and without small amounts of fluorine and phosphorus, structural (specific surface area, structure resistance coefficient, and mean pore radius) and electrosurface characteristics (adsorption of potential-determining ions, conductivity, counterion transport numbers, and electrokinetic potential) in sodium and potassium chloride solutions are compared. Results of measuring the equilibrium and transport characteristics of membranes are used to calculate the constants of surface reactions and adsorption potentials of ions within the framework of the 2-pK model of oxide surface charging. Within the framework of the homogeneous model, electrochemical characteristics of porous glasses, namely, concentrations of co- and counterions in a pore-confined liquid, Donnan potentials, convective component of the conductivity of a pore-confined solution, and mobility of counterions in the membranes, are calculated.__________Translated from Kolloidnyi Zhurnal, Vol. 67, No. 3, 2005, pp. 342–351.Original Russian Text Copyright © 2005 by Ermakova, Medvedeva, Volkova, Sidorova, Antropova.