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81.
Michele Petenzi Dr. Daniela Verga Dr. Eric Largy Dr. Florian Hamon Dr. Filippo Doria Dr. Marie‐Paule Teulade‐Fichou Aurore Guédin Dr. Jean‐Louis Mergny Prof. Mariella Mella Prof. Mauro Freccero 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(45):14487-14496
We report herein a solvent‐free and microwaved‐assisted synthesis of several water soluble acyclic pentaheteroaryls containing 1,2,4‐oxadiazole moieties ( 1 – 7 ). Their binding interactions with DNA quadruplex structures were thoroughly investigated by FRET melting, fluorescent intercalator displacement assay (G4‐FID) and CD spectroscopy. Among the G‐quadruplexes considered, attention was focused on telomeric repeats together with the proto‐oncogenic c‐kit sequences and the c‐myc oncogene promoter. Compound 1 , and to a lesser extent 2 and 5 , preferentially stabilise an antiparallel structure of the telomeric DNA motif, and exhibit an opposite binding behaviour to structurally related polyoxazole ( TOxaPy ), and do not bind duplex DNA. The efficiency and selectivity of the binding process was remarkably controlled by the structure of the solubilising moieties. 相似文献
82.
Guirgis BS Sá e Cunha C Gomes I Cavadas M Silva I Doria G Blatch GL Baptista PV Pereira E Azzazy HM Mota MM Prudêncio M Franco R 《Analytical and bioanalytical chemistry》2012,402(3):1019-1027
The development of rapid detection assays for malaria diagnostics is an area of intensive research, as the traditional microscopic analysis of blood smears is cumbersome and requires skilled personnel. Here, we describe a simple and sensitive immunoassay that successfully detects malaria antigens in infected blood cultures. This homogeneous assay is based on the fluorescence quenching of cyanine 3B (Cy3B)-labeled recombinant Plasmodium falciparum heat shock protein 70 (PfHsp70) upon binding to gold nanoparticles (AuNPs) functionalized with an anti-Hsp70 monoclonal antibody. Upon competition with the free antigen, the Cy3B-labeled recombinant PfHsp70 is released to solution resulting in an increase of fluorescence intensity. Two types of AuNP-antibody conjugates were used as probes, one obtained by electrostatic adsorption of the antibody on AuNPs surface and the other by covalent bonding using protein cross-linking agents. In comparison with cross-linked antibodies, electrostatic adsorption of the antibodies to the AuNPs surfaces generated conjugates with increased activity and linearity of response, within a range of antigen concentration from 8.2 to 23.8 μg.mL(-1). The estimated LOD for the assay is 2.4 μg.mL(-1) and the LOQ is 7.3 μg.mL(-1). The fluorescence immunoassay was successfully applied to the detection of antigen in malaria-infected human blood cultures at a 3% parasitemia level, and is assumed to detect parasite densities as low as 1,000 parasites.μL(-1). 相似文献
83.
Aldo Tava Elisa Biazzi Domenico Ronga Mariella Mella Filippo Doria Trifone DAddabbo Vincenzo Candido Pinarosa Avato 《Molecules (Basel, Switzerland)》2021,26(15)
Sulla (Hedysarum coronarium L.) is a biennal forage legume originated from the Mediterranean basin and used for animal feeding due to its high forage quality and palatability. Several species of Hedysarum have been considered for their nutritional, pharmaceutical, and biological properties, and different applications have been reported, both for human consumption and animal nutrition. Although a systematic investigation of the chemical constituents of Hedysarum spp. has been performed in order to provide chemotaxonomic evidences for the genus and to support the pharmacological application of several species within the genus, few data are available on the chemical constituents of H. coronarium, and only the content of condensed tannins and flavonoids in leaves has been previously reported. In the present paper, results from a detailed chemical analysis of the extracts from the leaves and flowers of H. coronarium grown wild in southern Italy are presented. Identification of the main specialized metabolites within the chemical classes of flavonoids, proanthocyanidins and saponins, is described, including considerations on their content in the two plant organs. Information acquired from this study expands the knowledge on H. coronarium as a source of valuable phytochemicals for different applications in human and animal health and nutrition. 相似文献
84.
Irradiation of 2-alkynylphenols resulted in the generation of vinylidene-quinone methides (QMs), which were detected by laser flash photolysis in organic solvents and aqueous acetonitrile. QMs' spectroscopic properties and electrophilicity were both significantly affected by β-silicon effect. The hydration of the alkynyl moiety (22 and 900 M(-1) s(-1)for QM-1 and QM-2, in aqueous acetonitrile) was an acid- and base-catalyzed process. The addition of amines was fast (9.2 × 10(3) M(-1) s(-1) < k(2) < 1.3 × 10(8) M(-1) s(-1)), yielding ketimines, with primary amines. 相似文献
85.
Serena Doria 《Annals of Operations Research》2012,195(1):33-48
A model of coherent upper conditional prevision for bounded random variables is proposed in a metric space. It is defined
by the Choquet integral with respect to Hausdorff outer measure if the conditioning event has positive and finite Hausdorff
outer measure in its Hausdorff dimension. Otherwise, when the conditioning event has Hausdorff outer measure equal to zero
or infinity in its Hausdorff dimension, it is defined by a 0–1 valued finitely, but not countably, additive probability. If
the conditioning event has positive and finite Hausdorff outer measure in its Hausdorff dimension it is proven that a coherent
upper conditional prevision is uniquely represented by the Choquet integral with respect to the upper conditional probability
defined by Hausdorff outer measure if and only if it is monotone, comonotonically additive, submodular and continuous from
below. 相似文献