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51.
A Combined NMR and Computational Approach to Determine the RGDechi‐hCit‐αvβ3 Integrin Recognition Mode in Isolated Cell Membranes
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Dr. Biancamaria Farina Dr. Ivan de Paola Dr. Luigi Russo Dr. Domenica Capasso Dr. Annamaria Liguoro Dr. Annarita Del Gatto Dr. Michele Saviano Prof. Paolo V. Pedone Dr. Sonia Di Gaetano Dr. Gaetano Malgieri Dr. Laura Zaccaro Prof. Roberto Fattorusso 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(2):681-693
The critical role of integrins in tumor progression and metastasis has stimulated intense efforts to identify pharmacological agents that can modulate integrin function. In recent years, αvβ3 and αvβ5 integrin antagonists were demonstrated to be effective in blocking tumor progression. RGDechi‐hCit, a chimeric peptide containing a cyclic RGD motif linked to an echistatin C‐terminal fragment, is able to recognize selectively αvβ3 integrin both in vitro and in vivo. High‐resolution molecular details of the selective αvβ3 recognition of the peptide are certainly required, nonetheless RGDechi‐hCit internalization limited the use of classical in cell NMR experiments. To overcome such limitations, we used WM266 isolated cellular membranes to accomplish a detailed NMR interaction study that, combined with a computational analysis, provides significant structural insights into αvβ3 molecular recognition by RGDechi‐hCit. Remarkably, on the basis of the identified molecular determinants, we design a RGDechi‐hCit mutant that is selective for αvβ5 integrin. 相似文献
52.
53.
B. W. van Oudheusden F. Scarano N. P. van Hinsberg D. W. Watt 《Experiments in fluids》2005,39(1):86-98
The vortex formation and shedding process in the near wake region of a 2D square-section cylinder at incidence has been investigated by means of particle image velocimetry (PIV). Proper orthogonal decomposition (POD) is used to characterize the coherent large-scale flow unsteadiness that is associated with the wake vortex shedding process. A particular application of the POD analysis is to extract the vortex-shedding phase of individual velocity fields, which were acquired at asynchronous low rate with respect to the vortex shedding cycle. The phase of an individual flow field is determined from its projection on the first pair of POD modes, allowing phase averaging of the measurement data to be performed. In addition, a low-order representation of the flow, constructed from the mean and the first pair of POD modes, is found to be practically equivalent to the phase-averaged results. It is shown that this low-order representation corresponds to the basic Fourier component of the flow field ensemble with respect to the reconstructed phase. The phase-averaged flow representations reveal the dominant flow features of the vortex-shedding process and the effect of the angle of incidence upon it. 相似文献
54.
Stefano Sfarra Clemente Ibarra-Castanedo Stefano Ridolfi Giorgio Cerichelli Dario Ambrosini Domenica Paoletti Xavier Maldague 《Applied Physics A: Materials Science & Processing》2014,115(3):1041-1056
Wood has been routinely employed in decorative arts, as well as in sculptures and paintings (support) during the Middle Ages, because of its unique aesthetic virtues. It may safely be assumed that wood, as a material for monumental sculpture, was much more commonly employed in the mediaeval period than existing examples would seem to indicate (Bulletin of the metropolitan Museum of Art, 2013). Wood is easily obtainable; it could be carved and put in place with less difficulty than stone, it is chemically stable when dry, and its surface offers a compatible substrate for paint application. However, the use of wood is not without pitfalls, and requires an understanding of its anisotropic and hygroscopic nature. It is also dimensionally unstable and subject to deterioration by fungi and insects. Moisture-related dimensional changes are certainly among the most challenging problems in painting conservation. With the purpose of preventing important damages, the use of non-or microdestructive testing (NDT) techniques is undoubtedly of paramount interest for painted wooden statues of great value. This work has a threefold purpose: (1) to validate the effectiveness of an integrated approach using near-infrared (NIR) reflectography, square pulse thermography (SPT), and holographic interferometry (HI) techniques for discovering old repairs and/or inclusions of foreign materials in a wooden structure, (2) to confirm and approximately date the restoration carried out by x-ray fluorescence (XRF) spectroscopy and energy-dispersive x-ray spectroscopy (EDS) (that is assembled with a scanning electron microscopy—SEM) techniques, and (3) to combine into a multidisciplinary approach two quantitative NDT results coming from optical and thermographic methods. The subject of the present study was a statue named “Virgin with her Child” (XIV century), whose origins are mysterious and not properly documented. 相似文献
55.
Anizan S Di Nardo D Bichon E Monteau F Cesbron N Antignac JP Le Bizec B 《Analytica chimica acta》2011,700(1-2):105-113
The use of steroid hormones as growth promoters in cattle is banned within the European Union since 1988 but can still be fraudulently employed in animal breeding farms for anabolic purposes. While efficient targeted confirmatory methods have been implemented in control laboratories for many years, fast and reliable screening methods are still required, especially in the case of natural hormones abuse, but more globally for new "fishing" strategies allowing to reveal the use of even unknown anabolic agents. The development of focused profiling or untargeted metabolomic approaches is thus emerging in this context. The present study was a focused profiling study using steroids phase II metabolites, with the aim to get a better understanding of the steroid metabolism disruptions after exogenous administration of androstenedione and finally reveal potential biomarkers signing its administration. A sample preparation procedure was first developed, based on a separation of 31 glucuronide and sulphate conjugate compounds using an anion exchange SPE system. Each fraction was then analysed by UPLC-MS/MS in MRM mode showing a rapid (between 4h and 4 days after treatment) and huge excretion of several direct metabolites of androstenedione such as etiocholanolone-glucuronide or epiandrosterone-sulphate. 相似文献
56.
Sophie Ayrault Laurence Galsomiès GÉraldine Amblard Maria Domenica Sciarretta Patricia Bonhomme André Gaudry 《International journal of environmental analytical chemistry》2013,93(7):463-473
Two analytical methods - instrumental neutron activation analysis (INAA) and inductively coupled plasma mass spectrometry (ICP-MS) - were used for the trace element analysis of naturally growing mosses for a heavy metal biomonitoring survey. The techniques were applied to reference mosses to evaluate the feasibility, analytical variability, detection limits and accuracy. These parameters were evaluated using 563 mosses sampled in the 1996 French survey. All the elements of interest in the European program "Atmospheric Heavy Metal Deposition in Europe - estimation based on moss analysis" (As, Cd, Cr, Cu, Fe, Hg, Pb, Ni, V, Zn) were able to be determined by ICP-MS. INAA appeared suitable for the determination of As, Cr, Fe, Hg, V and Zn. The Cd, Cu, Ni and Pb concentrations determined by ICP-MS were preferred to the INAA results, because of increased feasibility or accuracy. The results provided by both methods on the French mosses were statistically compared for 14 elements. Significant linear correlation appeared for: Ba, Ce, Cs, La, Rb, Sm, Th and V. Among these eight elements, Ba, Cs, La and Sm concentrations determined by both methods exhibited a strong statistical similarity. The correlations obtained for As, Eu, Fe and Sb were not as strong and no correlation at all was observed for Co and Cr. These differences were attributed to instrumental factors (e.g. spectral interference occurred for both methods) or due to the sample preparation prior to ICP-MS. The consequences of such results on the regional trend evaluation of atmospheric heavy metal deposition were discussed. 相似文献
57.
Lorella Guadagnini Marco Giorgetti Domenica Tonelli 《Journal of Solid State Electrochemistry》2013,17(11):2805-2814
A glucose amperometric biosensor was developed. Glucose oxidase enzyme was immobilized by means of a Nafion membrane on glassy carbon modified with an electrochemically deposited mixed Cu and Pd hexacyanoferrate (CuPdHCF). According to the data provided by X-ray atomic spectroscopy measurements, this Cu- and Pd-based hexacyanoferrate is likely to be a mixture of single CuHCF and PdHCF pure phases. The biosensor performances were evaluated by recording the steady-state currents due to submillimolar additions of glucose to a potassium buffer solution (pH 5.5) and exploiting the electrocatalytic reduction of the enzymatically produced hydrogen peroxide. The CuPdHCF-based biosensor exhibited a sensitivity of 8.1?±?0.6 A M?1 m?2, a limit of detection of 1.4?×?10?5 M, and a linear response range extending between 5?×?10?5 and 4?×?10?4 M, with a dynamic response range up to 4?×?10?3 M glucose. Electrode sensitivity and signal stability resulted more satisfactory as compared to those of a CuHCF-based biosensor fabricated according to the same procedure. The selectivity was investigated through an interference study. The response to easily oxidizable species was found to be low enough to allow glucose determination in biological samples. 相似文献
58.
Stefano Mariani Maria Laura Ermini Simona Scarano Francesca Bellissima Massimo Bonini Debora Berti Maria Minunni 《Mikrochimica acta》2013,180(11-12):1093-1099
The use of nanoparticles (NPs) can substantially improve the analytical performance of surface plasmon resonance imaging (SPRi) in general, and in DNA sensing in particular. In this work, we report on the modification of the gold surface of commercial biochips with gold nanospheres, silica-coated gold nanoshells, and silver nanoprisms, respectively. The NPs were tethered onto the surface of the chip and functionalized with a DNA probe. The effects of tethering conditions and varying nanostructures on the SPRi signals were evaluated via hybridization assays. The results showed that coupling between planar surface plasmons and electric fields, generated by localized surface plasmons of the NPs, is mandatory for signal enhancement. Silver nanoprisms gave the best results in improving the signal change at a target DNA concentration of <50 nM by +50 % (compared to a conventional SPRi chip). The limit of detection for the target DNA was 0.5 nM which is 5 times less than in conventional SPRi. Figure
? 相似文献
59.
Domenica Scarano Serena Bertarione Federico Cesano Giuseppe Spoto Adriano Zecchina 《Surface science》2004,570(3):155-166
Atomic force microscopy in contact, non-contact and in high resolution modes have been used to image MgO powder samples, obtained at different degree of sintering, starting from Mg(OH)2 decomposition or obtained in form of smoke. From high resolution AFM images of MgO smoke, the lattice periodicity on regular surfaces has been revealed for the first time, under ambient conditions. The high surface perfection of the microcrystals has been further confirmed by HRTEM analysis. To obtain more information on the local structure of the single faces, in terms of type and distribution of the surface active sites, the adsorption of a simple probe molecule (CO) on such surfaces has been investigated by means of FTIR spectroscopy. 相似文献
60.
Assessment of the Antioxidant Capacity of Standard Compounds and Fruit Juices by a Newly Developed Electrochemical Method: Comparative Study with Results from Other Analytical Methods
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Isacco Gualandi Luca Ferraro Pietro Matteucci Domenica Tonelli 《Electroanalysis》2015,27(8):1906-1914
The antioxidant capacity (AOC) of some standard compounds and commercial beverages was determined using an electroanalytical method which measures the OH radical scavenging ability (OH‐RSC). It is based on the degradation of a thin polyphenol film electrodeposited on glassy carbon following the attack by OH radicals produced by hydrogen peroxide photolysis. The degradation is prevented in presence of increasing concentrations of antioxidants. The same samples were also analyzed by DPPH, ABTS, and ORAC assays. The OH‐RSC method provided AOC values highly correlated with those resulting from the application of ORAC assay for both juices (R=0.90) and standard antioxidants (R=0.96). 相似文献