总被引：1，自引：0，他引：1  

Buse C  Altmann F  Amann B  Hauck SM  Poulsen Nautrup C  Ueffing M  Stangassinger M  Deeg CA 《Electrophoresis》2008,29(6):1325-1332

There is increasing evidence that a large proportion of dilated cardiomyopathy (DCM) cases are mediated by autoimmune processes. Since DCM is a fatal disorder with rapid aggravation and is the leading cause of heart transplantation, further insights into disease pathogenesis are needed. Recent studies have separated the pathogenic capacity of autoantibodies and initial clinical trials removing such autoantibodies via immunoadsorption have been promising. In order to elucidate the full autoantibody repertoire involved in DCM, we applied an autoantibody screening test using ventricular and atrial proteomes as autoantigenic sources and subsequently tested the autoantibody-binding patterns of sera from dogs with spontaneous DCM. With this method, we detected five potentially DCM-related autoantigens which were identified by MS as being: myosin heavy chain cardiac muscle alpha isoform, alpha cardiac actin, mitochondrial aconitate hydratase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and brain glycogen phosphorylase (GPBB). The recovery of two known DCM autoantigens (myosin heavy chain and alpha cardiac actin) and the discovery of three novel autoantigens (mitochondrial aconitate hydratase, GADPH, and GPBB) underscore the efficacy of this experimental method and the significance of the spontaneous canine DCM model.  相似文献   

    

Torsten Meyer  Thomas Hellweg  Stefan Spange  Stephanie Hesse  Christian Jger  Cornelia Bellmann 《Journal of polymer science. Part A, Polymer chemistry》2002,40(18):3144-3152

Polyvinylamine hydrogels with silica particles encapsulated (PVAm/silica) were produced by a two‐step synthesis. In the first step, polyvinylformamide/silica (PVFA/silica) hybrids were synthesized from vinylformamide (VFA) and 1,3‐divinylimidazolidin‐2‐one (1,3‐bisvinylethyleneurea, BVU), as the crosslinker, by radical copolymerization in silica/water suspensions using different compositions of VFA/BVU. The target product PVAm/silica was obtained by acidic hydrolysis of the PVFA/silica hydrogels in a second step. The chemical structures of both hydrogels, PVFA/silica and PVAm/silica, respectively, were revealed by solid‐state ¹³C(¹H) cross‐polarity/magic‐angle spinning NMR spectroscopy. Both hydrogels swelled significantly in water. The swelling capacity of the two systems was characterized by the correlation length ξ (or hydrodynamic blob size) of the network meshes with small‐angle neutron scattering experiments. ξ is significantly larger for PVAm/silica than for PVFA/silica, which corresponds to the observed higher swelling capacity of this polyelectrolyte material. Furthermore, the swelling behavior of the hybrid hydrogels was quantitatively described in terms of free swell capacity, centrifuge‐retention capacity, adsorption against pressure, and free swell rate as compared with values of the corresponding copolymer hydrogels. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 3144–3152, 2002  相似文献   

    

van Bommel KJ  van der Pol C  Muizebelt I  Friggeri A  Heeres A  Meetsma A  Feringa BL  van Esch J 《Angewandte Chemie (International ed. in English)》2004,43(13):1663-1667

  相似文献   

    

Ede Kapuy  Cornelia Kozmutza  Raymond Daudel 《Theoretical chemistry accounts》1980,56(4):259-267

The transferability of the first and second order electric moments of localized orbitals have been shown. Some moment characteristics have also been investigated. The standard deviation of first order localized moments is less than 2.0%. The standard deviation of second order electric moment components are larger: although they do not exceed 4.0% for bond orbitals, for lone pair ones they vary about 1.5–10.1%.  相似文献   

    

Ede Kapuy  Cornelia Kozmutza  Raymond Daudel  Michael E. Stephens 《Theoretical chemistry accounts》1979,53(2):147-157

The localized molecular orbitals of some related ten- and eighteen-electron systems have been studied. The transferability of the kinetic, self-interaction, Coulomb and exchange interaction energies on localized orbitals have been shown. The standard deviation of the kinetic and of the interaction energies (including exchange) are less than 2.5% except for lone pair orbitals of the oxygen atoms where the standard deviation is close to 4%.  相似文献   

    

Dr. Davide Audisio  Dr. Gopinadhanpillai Gopakumar  Dr. Lan‐Gui Xie  Dr. Luís G. Alves  Cornelia Wirtz  Prof. Dr. Ana M. Martins  Prof. Dr. Walter Thiel  Dr. Christophe Farès  Dr. Nuno Maulide 《Angewandte Chemie (International ed. in English)》2013,52(24):6313-6316

  相似文献   

    

Cornelia Meinert  Jan Hendrik Bredehft  Jean‐Jacques Filippi  Yannick Baraud  Laurent Nahon  Frank Wien  Nykola C. Jones  Sren V. Hoffmann  Uwe J. Meierhenrich 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2012,124(18):4562-4565

  相似文献   

    

Cornelia Wiegand  Uta-Christina Hipler 《Macromolecular Symposia》2010,294(2):1-13

Chronic wounds, such as venous, pressure, and diabetic ulcers, are difficult to heal and represent a rising social and economical problem. Compared to acute wounds, non-healing wounds contain elevated levels of neutrophil elastase, pro-inflammatory cytokines (IL-1β, IL-6, IL-8), and matrix metalloproteases (MMP-2, MMP-9, MMP-13) as well as free radicals. Their overproduction perpetuates the inflammatory phase resulting in severe tissue damage and degradation of growth factors. Consequently, wound closure is prevented and the wound remains non-healing for month or even years. The increasing numbers of patients suffering from wounds that fail to heal are a significant challenge for health care professionals. Wound dressings play an important role in the entire management of these wounds. New materials and treatment strategies are needed to improve wound care. Recent advances in the field of biomaterials and their medical applications indicate the significance and potential of various natural polymers in the development of novel classes of wound dressings. Native polymers are an ideal source for bio-active wound dressings because of their availability and biocompatibility. Hence, several studies have been conducted to explore the influence of wound dressings consisting of collagen, oxidized regenerated cellulose, bacterial cellulose, chitosan, or alginate on the destructive milieu in chronic wounds.  相似文献   

    

Uwe J. Meierhenrich Prof. Dr.  Jean‐Jacques Filippi Dr.  Cornelia Meinert  Pierre Vierling Dr.  Jason P. Dworkin Dr. 《Angewandte Chemie (International ed. in English)》2010,49(22):3738-3750

Recent major discoveries in membrane biophysics hold the key to a modern understanding of the origin of life on Earth. Membrane bilayer vesicles have been shown to provide a multifaceted microenvironment in which protometabolic reactions could have developed. Cell‐membrane‐like aggregates of amphiphilic molecules capable of retaining encapsulated oligonucleotides have been successfully created in the laboratory. Sophisticated laboratory studies on the origin of life now show that elongation of the DNA primer takes place inside fatty acid vesicles when activated nucleotide nutrients are added to the external medium. These studies demonstrate that cell‐like vesicles can be sufficiently permeable to allow for the intake of charged molecules such as activated nucleotides, which can then take part in copying templates in the protocell interior. In this Review we summarize recent experiments in this area and describe a possible scenario for the origin of primitive cells, with an emphasis on the elongation of encapsulated nucleotides.  相似文献   

    

Cornelia M. Mömming  Gerald Kehr Dr.  Birgit Wibbeling  Roland Fröhlich Dr.  Birgitta Schirmer  Stefan Grimme Prof. Dr.  Gerhard Erker Prof. Dr. 《Angewandte Chemie (International ed. in English)》2010,49(13):2414-2417

  相似文献