Development and thermodynamic evaluation of novel lipid raft stationary phase chromatography for screening potential antitumor agents

Novel lipid raft stationary phase chromatography (LRSC), with lipid rafts that contain abundant tropomyosin‐related tyrosine kinase A receptors immobilized on the stationary phase, was developed for a high‐throughput screening of potentially active antitumor agents. Lestaurtinib was used as a model compound to determine the operational parameters of the LRSC. Of all the factors considered, the particle size of column packing, the column temperature and the flow rate were of immense importance in determining the performance of the established LRSC system. In order to profoundly comprehend the binding interaction between the model drug and the receptors on the column, thermodynamic studies were employed. The results revealed that the interaction was spontaneous and exothermic, a typical enthalpy‐driven process. Additionally, the primary forces were hydrogen bonding and van der Waals forces. In evaluating the applicability of the method, active extracts from Albizziae Cortex were screened out using the LRSC system under the optimized conditions. The bioactive components were successfully confirmed by the MTT assay. In conclusion, it could be said that the LRSC is a good model for screening potential antitumor agents because of its viability, rapid response and scalable features. Copyright © 2014 John Wiley & Sons, Ltd.     

连续化方法求解变分不等式问题

利用变分不等式问题的KKT条件,给出了连续化方法求解变分不等式问题的一般框架,该框架包含了现存的几种连续方法;并给出一种求解的基本算法,证明了基本算法的可行性及算法的收敛性;最后用数值试验验证了算法的稳定性和有效性。     

Simultaneous HPLC determination of ergosterol and 22,23‐dihydroergosterol in Flammulina velutipes sterol‐loaded microemulsion

An efficient HPLC method was developed and validated for the simultaneous determination of ergosterol and 22,23‐dihydroergosterol in Flammulina velutipes sterol‐loaded microemulsions (FVSMs). The different chromatographic conditions for in vitro and in vivo determinations were investigated, with the application examined by tissue distribution. Chromatographic separation was achieved on an Inertsil ODS‐SP (250 × 4.6 mm, 5 µm) analytical column using a mobile phase of 98% methanol (in vitro), and 93% methanol for stomach samples and 96% methanol for other samples (in vivo) at 1.0 mL/min. The sterol content was detected at 282 nm. The established in vitro linearity ranges for ergosterol and 22,23‐dihydroergosterol were 0.58–72.77 µg/mL (r1 = 0.9999) and 0.59–73.25 µg/mL (r2 = 0.9999), respectively, with the biological (in vivo) samples following the same trend. The accuracy of the method was >99% (in vitro) and between 93%–108% (in vivo). The LOQ was 2.15 µg/L for ergosterol and 2.41 µg/L for 22,23‐dihydroergosterol in the in vitro studies. Also, the precisions met the acceptance criterion. These results indicate that the established HPLC method was specific, linear, accurate, precise and sensitive for the separation and simultaneous determination of ergosterol and 22,23‐dihydroergosterol. Copyright © 2013 John Wiley & Sons, Ltd.     

Asymptotic expansions of Bessel, Anger and Weber transformations

In this paper we explore higher order numerical quadrature for the integrations of systems containing Bessel, Anger and Weber functions. The method is constructed by finding the approximate solution of the differential equation and truncating the asymptotic series. Numerical examples based on theoretical results are presented to demonstrate the efficiency of the method.     

人淋巴细胞在低温保存过程中超微结构的变化

在液氮内冻存的人外周血淋巴细胞,当冻存介质内不含低温保护剂时,细胞内呈现大量冰腔,细胞质、细胞核、细胞器和生物膜结构发生严重破坏.细胞的结冰是造成细胞死亡和功能丧失的初始原因.当冻存在含有10％二甲基亚砜或甘油的介质内,40天时,绝大部分淋巴细胞具有完好的超微结构,少量细胞遭受冷冻损伤,主要表现在细胞器.如线粒体膨胀、(?)结构破坏等.冻存一年时,部分细胞的细胞核形状和结构也发生了深刻的变化,如细胞核分叶,核膜呈锯齿状皱缩,染色质分布不匀等.     

烷氧基锌酞菁的二聚性质研究

四吡咯环的金属络合物(如叶绿素、脱镁叶绿素等)是植物中构成光合作用反应中心的重要化合物,它们通过光诱导电子转移过程产生高效率的电荷分离态^[1]。 .     

Temporal Pulse Shaping of Laser Beams by Optical Fiber Stacker

１ＩｎｔｒｏｄｕｃｔｉｏｎＰｒｅｃｉｓｅｔｅｍｐｏｒａｌｐｕｌｓｅｓｈａｐｉｎｇｏｆｔｈｅｌａｓｅｒｂｅａｍｓｆｏｒｉｎｅｒｔｉａｌｃｏｎｆｉｎｅｍｅｎｔｆｕｓｉｏｎ（ＩＣＦ）ｅｘｐｅｒｉｍｅｎｔｓｈａｓｂｅｃｏｍｅｉｎｃｒｅａｓｉｎｇｉｍｐｏｒｔ...     

二极管端面泵浦的附加脉冲锁模的Nd：YLF激光器

研究了二极管端面泵浦的附加脉冲锁模的Ｎｄ：ＹＡＧ激光器并分析了脉冲压缩原理及自启动机制,在最大泵浦功率为２．４Ｗ时,得到重复频率为１３４ＭＨｚ、脉宽为２ｐｓ、有效输出功率７６ｍＷ、相应峰值继２８３Ｗ、波长为１．０５３μｍ、波长为１．０５３μｍ的稳定的连续模脉冲序列。     

可用于建筑结构检测的分布式光纤形变片

本文在光时域反射计(OTDR)和光纤形变片的基础上,提出了一种分布式光纤传感器-分布式光纤形变片.该检测方法属于OTDR的损耗调制法,即通过测量粘贴于光纤形变片上的光纤弯曲损耗来获取测量点的应变量或位移量.作者分别在微位移架和悬臂梁进行了位移检测和应变检测,结果表明该分布式光纤形变片提供了一种可检测应变量与位移量的检测方法.值得注意的是,该分布式光纤形变片的传感网络采用时分复用的总线拓扑,能在一根光纤上同时检测多个检测点的变化.