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排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Microencapsulation of DNA Within alginate microspheres and crosslinked chitosan membranes for in vivo application 总被引:4,自引:0,他引:4
T. Alexakis D. K. Boadi D. Quong A. Groboillot I. O’Neill D. Poncelet R. J. Neufeld 《Applied biochemistry and biotechnology》1995,50(1):93-106
Calf thymus DNA was microencapsulated within crosslinked chitosan membranes, or immobilized within chitosan-coated alginate
microspheres. Microcapsules were prepared by interfacial polymerization of chitosan, and alginate microspheres formed by emulsification/
internal gelation. Diameters ranged from 20 to 500 Μm, depending on the formulation conditions. Encapsulated DNA was quantifiedin situ by direct spectrophotometry (260 nm) and ethidium bromide fluorimetry, and compared to DNA measurements on the fractions
following disruption and dissolution of the microspheres. Approximately 84% of the DNA was released upon core dissolution
and membrane disruption, with 12% membrane bound. The yield of encapsulation was 96%. Leakage of DNA from intact microspheres/capsules
was not observed. DNA microcapsules and microspheres were recovered intact from rat feces following gavage and gastrointestinal
transit. Higher recoveries (60%) and reduced shrinkage during transit were obtained with the alginate microspheres. DNA was
recovered and purified from the microcapsules and microspheres by chromatography and differential precipitation with ethanol.
This is the first report of microcapsules or microspheres containing biologically active material (DNA) being passed through
the gastrointestinal tract, with the potential for substantial recovery. 相似文献
992.
We study the conjugate boundary-value problem with boundary conditions whose orders can be as large as the order of the differential equation or larger. We construct the Green's function of the problem over the entire space when the separating surface is a hyperplane, and we describe its properties. Bibliography: 6 titles.Translated fromMatematicheskie Metody i Fiziko-Mekhanicheskie Polya, Issue 30, 1989, pp. 20–24. 相似文献
993.
The microwave subsidiary absorption threshold in tangentially magnetized yttrium-iron-garnet (YIG) films was measured as a function of the static magnetic field, film thickness and decrease in the 0.3–3 μm range is observed. The effect is explained quantitatively by using modified Damon-Eshbach dispersion relations which take into account exchange interactions.
The effect of temperature on hcrit, the smallest threshold field amplitude, in the 250<T< 450 K temperature range can be expressed by an empirical relationship hcrit Ms(T)-1.6 where Ms is the saturation magnetization. This hcrit vs Ms relationship is similar to that obtained for the magnetization dependence of parallel pump thresholds in Li-Ti ferrite compounds. 相似文献
994.
Z. I. Simenog 《Ukrainian Mathematical Journal》1996,48(9):1465-1469
Parasupersymmetric quantum mechanics is generalized to the case of an arbitrary number of parasuperchanrgesN and the order of paraquantizationp. We show that parasuperpotentials can be explicitly expressed via a single arbitrary function.
Institute of Mathematics, Ukrainian Academy of Sciences, Kiev. Translated from Ukrainskii Matematicheskii Zhurnal, Vol. 48,
No. 9, pp. 1291–1294, September, 1996. 相似文献
995.
996.
D. V. Kazakov A. I. Voloshin N. N. Kabal'nova V. V. Shereshovets V. P. Kazakov 《Russian Chemical Bulletin》1996,45(10):2452-2453
Chemiluminescence (CL) was found upon the isomerization of dimethyldioxirane in the gas phase under argon atmosphere. The intensity of CL increases as temperature increases and decreases with time at constant temperature. If Silipor is placed in a cell containing the dimethyldioxirane vapor in argon, the intensity of CL sharply increases (more than 10 times) and then decreases following the exponential law. In all cases tripletly excited methyl acetate is the emitter of chemiluminescence.[/ p]Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 10, pp. 2582–2583, October 1996. 相似文献
997.
998.
Summary A rapid, robust and reproducible method providing excellent separation performance and simplicity using a 0.5% MC-4000 methyl
cellulosic sieving medium in DB-1 coated capillaries has been developed. The method is suitable for qualitative comparison
of DNA restriction profiles for fragments in the size range 100–1000 base pairs (bp). Efficiencies up to 8.5 million plates/m
(1057 bp fragment) were recorded. Peak resolution of 6 bp (291/297 bp, 335/341 bp) and 4 bp (238/242 bp, 341/345 bp) was achieved.
In addition, 1 bp partial resolution of 123/124 bp and 298/297 bp was obtained. Run-to-run (n=15), day-to-day (n=4), and capillary-to-capillary
(n=3) variations of 0.1–0.2% RSD, 0.3–0.5% RSD, and 0.1–0.3% RSD, respectively, were observed. The MC-4000 sieving matrix
was found to be better than hydroxypropyl methyl cellulose and hydroxypropyl cellulose, in terms of both performance and stability
in the DB-1 coated capillaries. The efficiency and resolution in DB-WAX capillaries were inferior to those obtained in DB-1
capillaries. The commercially available DB-1 capillaries were stable for months in the sieving medium at pH 8.3 and could
be regenerated to provide high efficiency after accidental current breaks. 相似文献
999.
1000.
ALEPH Collaboration D. Buskulic D. Casper I. De Bonis D. Decamp P. Ghez C. Goy J. -P. Lees A. Lucotte M. -N. Minard et al. 《Zeitschrift fur Physik C Particles and Fields》1995,69(1):379-392
A data sample of about 3.0 million hadronicZ decays collected by the ALEPH experiment at LEP in the years 1991 through 1994 is used to make an inclusive selection of
B hadron events. In this event sample 4227±140±252B* mesons in the decayB*→Bγ and 1944±108±161B**
u,d
mesons decaying into a B meson and a charged pion are reconstructed. Here and in the followingB**
u,d
denotes the eightL=1(bū) and (bd) states and their charge conjugate.
For the well establishedB* meson the following quantities are obtained:ΔM=M
B*−MB=(45.30±0.35±0.87) MeV/c2 andN
B*/(N
B+N
B*)=(77.1±2.6±7.0)%. The angular distribution of the photons in theB* rest frame is used to measure the relative contribution of longitudinalB* polarization states to beσ
L/(σ
L+σ
T)=(33±6±5)%. 相似文献