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排序方式: 共有92条查询结果,搜索用时 15 毫秒
41.
H Ding J.R EngelbrechtZ Wang S.-C WangH.-B Yang J.C Campuzano T TakahashiK Kadowaki D.G Hinks 《Journal of Physics and Chemistry of Solids》2002,63(12):2135-2139
We study the doping and temperature dependence of the single-particle coherent weight, zA, for high-Tc superconductors Bi2Sr2CaCu2O8+x using angle-resolved photoemission. We find that at low temperatures the coherent weight zA at (π,0) is proportional to the carrier concentration x and that the temperature-dependence of zA is similar to that of the c-axis superfluid density. We show that, for a wide range of carrier concentration, the superconducting transition temperature scales with the product of the low-temperature coherent weight and the maximum superconducting gap. 相似文献
42.
Vanessa Escamilla‐Gómez Susana Campuzano María Pedrero José M. Pingarrón 《Electroanalysis》2007,19(14):1476-1482
Amperometric immunosensors for the detection and quantification of S. aureus using MPA self‐assembled monolayer modified electrodes for the immobilization of the immunoreagents are reported. Two different immunosensor configurations were compared. A competitive mode, in which protein A‐bearing S. aureus cells and antiRbIgG labeled with horseradish peroxidase (HRP) compete for the binding sites of RbIgG immobilized onto the 3‐mercaptopropionic acid (MPA) modified electrode, was evaluated. Moreover, a sandwich configuration in which S. aureus cells were immobilized onto the MPA SAM, and RbIgG and antiRbIgG labeled with HRP were further linked to the electrode surface, was also tested. In both cases, TTF was used as the redox mediator of the HRP reaction with H2O2, and it was co‐immobilized onto the MPA‐modified gold electrode. After optimization of the working variables for both configurations, the analytical performance of the amperometric measurements carried out at 0.00 V (vs. Ag/AgCl) showed that the competitive immunosensor exhibited a lower limit of detection (1.6×105 S. aureus cells mL?1), as well as a better repeatability and reproducibility of the measurements. 相似文献
43.
A novel approach to construct an electrochemical DNA sensor based on immobilization of a 25 base single-stranded probe, specific to E. coli lac Z gene, onto a gold disk electrode is described. The capture probe is covalently attached using a self-assembled monolayer of 3,3′-dithiodipropionic acid di(N-succinimidyl ester) (DTSP) and mercaptohexanol (MCH) as spacer. Hybridization of the immobilized probe with the target DNA at the electrode surface was monitored by square wave voltammetry (SWV), using methylene blue (MB) as electrochemical indicator. Variables involved in the sensor performance, such as the DTSP concentration in the modification solution, the self-assembled monolayers (SAM) formation time, the DNA probe drying time atop the electrode surface and the amount of probe immobilized, were optimized.
A good stability of the single- and double-stranded oligonucleotides immobilized on the DTSP-modified electrode was demonstrated, and a target DNA detection limit of 45 nM was achieved without signal amplification. Hybridization specificity was checked with non-complementary and mismatch oligonucleotides. A single-base mismatch oligonucleotide gave a hybridization response only 7 ± 3%, higher than the signal obtained for the capture probe before hybridization. The possibility of reusing the electrochemical genosensor was also tested. 相似文献
44.
Vassilios Fessatidis Jay D. Mancini Samuel P. Bowen Mauricio Campuzano 《Journal of mathematical chemistry》2008,44(1):20-27
Here we wish to apply the newly developed Generalized Moments Expansion (GMX) to the well-known potential
which is used to model such molecular systems as formamide (HCONH2) and carbon suboxide (C3O2). Our motivation is to investigate the numerical accuracy as well as the viability of the GMX for evaluating ground-state
energies of quantum Hamiltonian systems. The zero-point energy of this potential is calculated and results are compared to
those of a related Canonical Sequence Method approach (CSM). 相似文献
45.
Óscar A. Loaiza Susana Campuzano María Pedrero José M. Pingaron 《Electroanalysis》2008,20(13):1397-1405
This paper describes specific electrochemical enterobacteriaceae lac Z gene DNA sensors based on immobilization of a thiolated 25 base single stranded probe onto disposable screen printed gold electrodes (gold SPEs). Two configurations have been evaluated. In the first one, the capture probe was attached to the electrode surface through its ? SH moiety, while mercaptohexanol (MCH) was used as spacer for the displacement of nonspecifically adsorbed oligonucleotide molecules. The hybridization event between the probe and target DNA sequences was detected at ?0.20 V by square‐wave voltammetry (SWV), using methylene blue (MB) as electrochemical indicator. The second genosensor configuration involved modification of gold high temperature SPEs with a 3,3′‐dithiodipropionic acid di(N‐succinimidyl ester) (DTSP) self‐assembled monolayer (SAM). Moreover, 2‐aminoethanol was used as blocking agent, and further modification with avidin allowed binding of the biotinylated enterobacteriaceae lac Z gene DNA probe. An enzyme amplified detection scheme was applied, based on the coupling of streptavidin‐peroxidase to the biotinylated complementary target, after the hybridization process, and immobilization of tetrathiafulvalene (TTF) as redox mediator atop the modified electrode. The amperometric response obtained at ?0.15 V after the addition of hydrogen peroxide was used to detect the hybridization process. Experimental variables concerning sensors composition and electrochemical transduction were evaluated in both cases. A better precision and reproducibility in the fabrication process, as well as a higher sensitivity were achieved using the biotinylated probe‐based sensor configuration. A limit of detection of 0.002 ng/μL was obtained without any preconcentration step. 相似文献
46.
Norman MR Kaminski A Rosenkranz S Campuzano JC 《Physical review letters》2010,105(18):189701; author reply 189702
47.
Direct Determination of miR‐21 in Total RNA Extracted from Breast Cancer Samples Using Magnetosensing Platforms and the p19 Viral Protein as Detector Bioreceptor 下载免费PDF全文
S. Campuzano E. López‐Hernández R. Granados J. M. Sánchez‐Puelles J. M. Pingarrón 《Electroanalysis》2014,26(10):2080-2087
A novel magnetobiosensing approach for the rapid, sensitive and selective miR‐21 detection is reported involving the use of a specific RNA probe (antimiR‐21), streptavidin‐magnetic beads (Strep‐MBs), the siRNA Binding Protein p19 as detector bioreceptor, and amperometric detection with the H2O2/hydroquinone (HQ) system at disposable screen‐printed carbon electrodes. The magnetosensor exhibited a dynamic range from 1.4 to 10 nM and a detection limit of 4.2 fmol of synthetic target miR‐21 without any amplification step in 75 min. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic breast cancer cell lines, human tissues and breast cytologies. 相似文献
48.
49.
Electrochemically-propelled nanomotors offer considerable promise for developing new and novel bioanalytical and biosensing strategies based on the direct isolation of target biomolecules or changes in their movement in the presence of target analytes. For example, receptor-functionalized nanomotors offer direct and rapid target isolation from raw biological samples without preparatory and washing steps. Microtube engines functionalized with ss-DNA, aptamer or antibody receptors are particularly useful for the direct isolation of nucleic acids, proteins or cancer cells, respectively. A new nanomotor-based signal transduction involving measurement of speed and distance travelled by nanomotors, offers highly sensitive, rapid, simple and low cost detection of target biomarkers, and a new dimension of analytical information based on motion. The resulting distance signals can be easily visualized by optical microscope (without any sophisticated analytical instrument) to reveal the target presence and concentration. The attractive features of the new micromachine-based target isolation and signal transduction protocols reviewed in this article offer numerous potential applications in biomedical diagnostics, environmental monitoring, and forensic analysis. 相似文献
50.
Cuyckens F Wassvik C Mortishire-Smith RJ Tresadern G Campuzano I Claereboudt J 《Rapid communications in mass spectrometry : RCM》2011,25(23):3497-3503
Travelling wave ion mobility spectrometry - mass spectrometry (TWIMS-MS) was evaluated as a tool for structural identification of metabolites of small molecule drugs in cases where the exact position of the biotransformation could not be identified by conventional tandem mass spectrometry. Test sets of compounds containing biotransformations at aromatic positions were analyzed. These present a problem for traditional MS methods since an atomic level localization of the biotransformation cannot normally be determined from MS(n) spectra. In addition to ion mobility measurements of the intact metabolite ions, ion mobility measurements of product ions were also made and the results compared with calculated values. This approach reduces the complexity of the problem, making theoretical calculations easier and more predictable when a modeled collision cross section (CCS) is required. A good relative correspondence between theoretical and measured CCSs was obtained allowing the identification of the exact position of the biotransformation. It was also demonstrated that authentic standards with substructures identical to those in the unknown can be used to assign the exact position of the biotransformation. In this approach the identification was based on the comparison of the drift times or CCSs for product ions of the standard, with those of the same product ions in the unknown. 相似文献