Lentivirus-mediated transgene delivery to the hippocampus reveals sub-field specific differences in expression

Background  

In the adult hippocampus, the granule cell layer of the dentate gyrus is a heterogeneous structure formed by neurons of different ages, morphologies and electrophysiological properties. Retroviral vectors have been extensively used to transduce cells of the granule cell layer and study their inherent properties in an intact brain environment. In addition, lentivirus-based vectors have been used to deliver transgenes to replicative and non-replicative cells as well, such as post mitotic neurons of the CNS. However, only few studies have been dedicated to address the applicability of these widespread used vectors to hippocampal cells in vivo. Therefore, the aim of this study was to extensively characterize the cell types that are effectively transduced in vivo by VSVg-pseudotyped lentivirus-based vectors in the hippocampus dentate gyrus.     

Analysis of transverse momentum and event shape invN scattering

The transverse momentum distributions of hadrons produced in neutrino-nucleon charged current interactions and their dependence onW are analysed in detail. It is found that the components of the transverse momentum in the event plane and normal to it increase withW at about the same rate throughout the availableW range. A comparison withe ⁺ e ^? data is made. Studies of the energy flow and angular distributions in the events classified as planar do not show clear evidence for high energy, wide angle gluon radiation, in contrast to the conclusion of a previous analysis of similar neutrino data.     

Coherent production of π+ mesons in ν-neon interactions

The coherent production of π⁺ mesons by charged current neutrino interactions on neon nuclei in BEBC is compared to the predictions of the PCAC hypothesis and the meson dominance model. Good agreement is observed.     

Baryon antibaryon production in the central region at 85 GeV/c

In a search for glueballs and exotic states decaying into baryons and antibaryons we have investigated the production of baryon antibaryon pairs produced centrally in the reactions π⁺/pp → π⁺/p(X ⁰)p at 85 GeV/c. In particular, channels whereX ⁰ goes to \(p\bar p,p\bar p\pi ^ + \pi ^ - ,p\bar p2\pi ^ + 2\pi ^ - \) and \(\Lambda \bar \Lambda \) have been observed. No significant new structure is observed in the mass spectra.     

Azimuthal anisotropy of photon and charged parti cle emissionin 208Pb + 208Pb collisions at 158 $\ cdot A$ GeV/ c

The azimuthal distributions of photons and charged particles with respect to the event plane are investigated as a function of centrality in ²⁰⁸Pb + ²⁰⁸Pb collisions at 158 GeV/c in the WA98 experiment at the CERN SPS. The anisotropy of the azimuthal distributions is characterized using a Fourier analysis. For both the photon and charged particle distributions the first two Fourier coefficients are observed to decrease with increasing centrality. The observed anisotropies of the photon distributions compare well with the expectations from the charged particle measurements for all centralities.Received: 22 May 2004, Revised: 14 April 2005, Published online: 4 May 2005PACS: 25.75.Dw     

Dilepton and trilepton production by antineutrinos and neutrinos in neon

A sample of over 25,000 fully measured neutrino and antineutrino charged current interactions in BEBC includes 192 dilepton candidates. The prompt signal after subtraction of background is 41 ±7µ⁺ e ^-, 35±7µ⁺µ^- events from \(\bar v\) interactions, and 32±7µ^-µ⁺ events from ν interactions. There are 2 trileptons, µ^-µ^- e ⁺ and µ^-µ^-µ⁺. Results are compared with other experimental data and with the standard model. Limits to prompt like sign µ⁺ e ⁺, µ⁺µ⁺ and µ^-µ^- signals are given and compared with other experiments and with theoretical calculations.     

Implementation of the moving particle semi-implicit method on GPU

The Moving Particle Semi-implicit (MPS) method performs well in simulating violent free surface flow and hence becomes popular in the area of fluid flow simulation. However, the implementations of searching neighbouring particles and solving the large sparse matrix equations (Poisson-type equation) are very time-consuming. In order to utilize the tremendous power of parallel computation of Graphics Processing Units (GPU), this study has developed a GPU-based MPS model employing the Compute Unified Device Ar...     

An adeno-associated viral vector transduces the rat hypothalamus and amygdala more efficient than a lentiviral vector

Background  

This study compared the transduction efficiencies of an adeno-associated viral (AAV) vector, which was pseudotyped with an AAV1 capsid and encoded the green fluorescent protein (GFP), with a lentiviral (LV) vector, which was pseudotyped with a VSV-G envelop and encoded the discosoma red fluorescent protein (dsRed), to investigate which viral vector transduced the lateral hypothalamus or the amygdala more efficiently. The LV-dsRed and AAV1-GFP vector were mixed and injected into the lateral hypothalamus or into the amygdala of adult rats. The titers that were injected were 1 × 10⁸ or 1 × 10⁹ genomic copies of AAV1-GFP and 1 × 10⁵ transducing units of LV-dsRed.