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971.
Dr. Enrico Ravera Silvia Ciambellotti Dr. Linda Cerofolini Dr. Tommaso Martelli Dr. Tatiana Kozyreva Dr. Caterina Bernacchioni Stefano Giuntini Prof. Marco Fragai Prof. Paola Turano Prof. Claudio Luchinat 《Angewandte Chemie (International ed. in English)》2016,55(7):2446-2449
PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic‐level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid‐state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use. 相似文献
972.
Marisa Taverna-Porro Claudio A. Pereira Adolfo M. Iribarren 《Tetrahedron letters》2008,49(16):2642-2645
Chemoenzymatic preparation of ribose, deoxyribose and arabinose 5-phosphates was accomplished. These compounds were tested as starting materials in the enzymatic preparation of natural and modified purine and pyrimidine nucleosides, using an overexpressed Escherichia coli phosphopentomutase. 相似文献
973.
Binolfi A Lamberto GR Duran R Quintanar L Bertoncini CW Souza JM Cerveñansky C Zweckstetter M Griesinger C Fernández CO 《Journal of the American Chemical Society》2008,130(35):11801-11812
The aggregation of alpha-synuclein (AS) is a critical step in the etiology of Parkinson's disease (PD) and other neurodegenerative synucleinopathies. Protein-metal interactions play a critical role in AS aggregation and might represent the link between the pathological processes of protein aggregation and oxidative damage. Our previous studies established a hierarchy in AS-metal ion interactions, where Cu(II) binds specifically to the protein and triggers its aggregation under conditions that might be relevant for the development of PD. In this work, we have addressed unresolved structural details related to the binding specificity of Cu(II) through the design of site-directed and domain-truncated mutants of AS and by the characterization of the metal-binding features of its natural homologue beta-synuclein (BS). The structural properties of the Cu(II) complexes were determined by the combined application of nuclear magnetic resonance, electron paramagnetic resonance, UV-vis, circular dichroism spectroscopy, and matrix-assisted laser desorption ionization mass spectrometry (MALDI MS). Two independent, noninteracting copper-binding sites with significantly different affinities for the metal ion were detected in the N-terminal regions of AS and BS. MALDI MS provided unique evidence for the direct involvement of Met1 as the primary anchoring residue for Cu(II) in both proteins. Comparative spectroscopic analysis of the two proteins allowed us to deconvolute the Cu(II) binding modes and unequivocally assign the higher-affinity site to the N-terminal amino group of Met1 and the lower-affinity site to the imidazol ring of the sole His residue. Through the use of competitive chelators, the affinity of the first equivalent of bound Cu(II) was accurately determined to be in the submicromolar range for both AS and BS. Our results prove that Cu(II) binding in the C-terminal region of synucleins represents a nonspecific, very low affinity process. These new insights into the bioinorganic chemistry of PD are central to an understanding of the role of Cu(II) in the fibrillization process of AS and have implications for the molecular mechanism by which BS might inhibit AS amyloid assembly. 相似文献
974.
Gasparrini F Pierini M Villani C Filippi A Speranza M 《Journal of the American Chemical Society》2008,130(2):522-534
The structure, stability, and reactivity of proton-bound diastereomeric [M x H x A]+ complexes between some amino acid derivatives (A) and several chiral tetra-amide macrocycles (M) have been investigated in the gas phase by ESI-FT-ICR and ESI-ITMS-CID mass spectrometry. The displacement of the A guest from the diastereomeric [M x H x A]+ complexes by reaction with the 2-aminobutane enantiomers (B) exhibits a distinct enantioselectivity with regards to the leaving amino acid A and, to a minor extent, to the amine reactant B. The emerging selectivity picture, discussed in the light of molecular mechanics calculations, provides compelling evidence that the most stable conformers of the selected chiral tetra-amide macrocycles M may acquire in the gas phase a different conformation by induced fit on complexation with some representative amino acid derivatives A. This leads to the coexistence in the gas phase of stable diastereomeric [M x H x A]+ eq-eq and ax-ax structures, in proportions depending on the configuration of A and M and characterized by different stability and reactivity toward the 2-aminobutane enantiomers. The enantioselectivity of the gas-phase A-to-B displacement in the diastereomeric [M x H x A]+ complexes essentially reflects the free energy gap between the homo- and heterochiral [M x H x A]+ complexes, except when the tetra-amidic host presents an additional macrocycle generated by a decamethylene chain. In this case, the measured enantioselectivity mostly reflects the stability difference between the relevant diastereomeric transition structures. 相似文献
975.
Sándor Kunsági-Máté Giuseppe Vasapollo Kornélia Szabó István Bitter Giuseppe Mele Luigia Longo László Kollár 《Journal of inclusion phenomena and macrocyclic chemistry》2008,60(1-2):71-78
The molecular encapsulation of functionalized fullerenes (substituted fulleropyrrolidines) with water-soluble calixarenes
was studied by photoluminescence and quantum-chemical methods. The results show that both the thiacalix[4]arene-tetrasulfonate
and calix[6]arene-hexasulfonate are able to overcome the natural water-repulsive character of fullerenes. However, the functionalization
of calixarenes and fullerenes induces significant changes in the molecular encapsulation processes, and the obtained thermodynamic
behavior of the complex formation highlights the importance of the entropy. Our results can contribute to the development
of the synthesis and design of functionalized calixarenes supporting their application in pharmaceutical and food chemistry. 相似文献
976.
Ramos JM Faget O G Felcman J Téllez S CA 《Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy》2008,71(4):1364-1370
The cysteinate glycinate cadmium(II) complex was synthesized and structural analysis was carried out using the following methods: determination of the C, H, N, S and O contents, thermogravimetry, infrared and Raman spectra. The most probable structure for the complex at a minimum of energy was calculated by the density functional theory (DFT):B3LYP/3-21G quantum mechanical method. The infrared and Raman spectra were analyzed and bands assigned through the DFT procedures, the stabilization energy being equal to: E(RB+HF-LYP)= -6442.67784a.u. Features of the infrared and Raman spectra confirm theoretical structural prediction with respect to the metal-ligand bonds: Cd-O, Cd-S and Cd-N. Full assignment of the vibrational spectra was also supported by a carefully analysis of the distorted geometries generated by the normal modes. 相似文献
977.
978.
Bergamot juice (BJ) contains different classes of flavonoids (e.g. flavanones and flavones) that can exert beneficial effects on human health. The aim of this study was to evaluate the qualitative and quantitative composition of a BJ obtained from fruits harvested in Southern Italy (Calabria) at the end of their maturation period. The identity of several flavonoids and furanocoumarins was assessed by co-chromatography, UV spectra and molecular weight comparison. The unknown compounds were dissociated by induced collision (CID-MS) and their identity established through the characteristic ions product. By this approach a complete profile of about twenty compounds (furano-coumarins, flavonoids C- and O-glycosides) present in BJ was obtained. Furthermore, three acylated flavanones, present in amounts of 20.1+/-1.1, 89.3+/-2.2 and 190.1+/-3.1 mg/L, respectively, and which seem to correspond to di-oxalate derivatives of neoeriocitrin, naringin and neohesperidin, were identified for the first time in BJ. The other main flavanones were naringin, neohesperidin and neoeriocitrin, and their content was 167.5+/-1.8, 123.9+/-1.7 and 73.3+/-1.6 mg/L, respectively. Concerning flavones, the total amount in BJ was about 160 mg/L and the main ones were vicenin-2, stellarin-2, rhoifolin and neodiosmin. Bergapten and bergamottin were the primary furanocoumarins in BJ and their amounts were 9.0+/-0.4 and 18.2+/-0.5 mg/L, respectively. 相似文献
979.
The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cmx4.6 mm id, 3 mum particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments. 相似文献
980.
Synthesis, redox, and amphiphilic properties of responsive salycilaldimine-copper(II) soft materials
Hindo SS Shakya R Rannulu NS Allard MM Heeg MJ Rodgers MT da Rocha SR Verani CN 《Inorganic chemistry》2008,47(8):3119-3127
Hydrolysis of the asymmetric pyridine- and phenol-containing ligand HL (1) (2-hydroxy-4-6-di- tert-butylbenzyl-2-pyridylmethyl)imine) led to the use of bis-(3,5-di -tert-butyl-2-phenolato-benzaldehyde)copper(II), [Cu (II)(L (SAL)) 2] ( 1) as a precursor for bis-(2,4-di- tert-butyl-6-octadecyliminomethyl-phenolato)copper(II), [Cu (II)(L (2)) 2] ( 3), bis-(2,4-di- tert-butyl-6-octadecyl aminomethyl-phenolato)copper(II), [Cu (II)(L (2A)) 2] ( 3'), and bis-(2,4-di- tert-butyl-6-[(3,4,5-tris-dodecyloxy-phenylimino)-methyl]-phenolato)copper(II), [Cu (II)(L (3)) 2] ( 4). These complexes exhibit hydrophilic copper-containing head groups, hydrophobic alkyl and alkoxo tails, and present potential as precursors for redox-responsive Langmuir-Blodgett films. All systems were characterized by means of elemental, spectrometric, spectroscopic, and electrochemical techniques, and their amphiphilic properties were probed by means of compression isotherms and Brewster angle microscopy. Good redox activity was observed for 3 with two phenoxyl radical processes between 0.5 and 0.8 V vs Fc (+)/Fc, but this complex lacks amphiphilic behavior. To attain good balance between redox response and amphiphilicity, increased core flexibility in 3' and incorporation of alkoxy chains in 4 were attempted. Film formation with collapse at 14 mN.m (-1) was observed for the alkoxy-derivative but redox-response was seriously compromised. Core flexibility improved Langmuir film formation with a higher formal collapse and showed excellent cyclability of the ligand-based processes. 相似文献