Oil phase evaporation-induced self-assembly of hydrophobic nanoparticles into spherical clusters with controlled surface chemistry in an oil-in-water dispersion and comparison of behaviors of individual and clustered iron oxide nanoparticles

We report a general method for preparing nanoparticle clusters (NPCs) in an oil-in-water emulsion system mediated by cetyl trimethylammonium bromide (CTAB), where previously only individual nanoparticles were obtained. NPCs of magnetic, metallic, and semiconductor nanoparticles have been prepared to demonstrate the generality of the method. The NPCs were spherical and composed of densely packed individual nanoparticles. The number density of nanoparticles in the oil phase was found to be critical for the formation, morphology, and yield of NPCs. The method developed here is scalable and can produce NPCs in nearly 100% yield at a concentration of 5 mg/mL in water, which is approximately 5 times higher than the highest value reported in the literature. The surface chemistry of NPCs can also be controlled by replacing CTAB with polymers containing different functional groups via a similar procedure. The reproducible production of NPCs with well-defined shapes has allowed us to compare the properties of individual and clustered iron oxide nanoparticles, including magnetization, magnetic moments, and contrast enhancement in magnetic resonance imaging (MRI). We found that, due to their collective properties, NPCs are more responsive to an external magnetic field and can potentially serve as better contrast enhancement agents than individually dispersed magnetic NPs in MRI.     

Precipitation and selective extraction of human serum endogenous peptides with analysis by quadrupole time-of-flight mass spectrometry reveals posttranslational modifications and low-abundance peptides

The endogenous peptides of human serum may have regulatory functions, have been associated with physiological states, and their modifications may reveal some mechanisms of disease. In order to correlate levels of specific peptides with disease alongside internal standards, the polypeptides must first be reliably extracted and identified. Endogenous blood peptides can be effectively enriched by precipitation of the serum with organic solvents followed by selective extraction of peptides using aqueous solutions modified with organic solvents. Polypeptides on filter paper were assayed with Coomasie brilliant blue binding. The polypeptides were resolved by detergent tricine polyacrylamide electrophoresis and visualized by diamine silver staining. Peptides in the extracts were collected by C18 and analyzed by matrix-assisted laser desorption/ionization and liquid chromatography–electrospray ionization–tandem mass spectrometry (MS/MS) quadrupole time-of-flight MS/MS. Peptides were resolved as multiple isotopic peaks in MS mode with mass deviation of 0.1 Da or less and similar accuracy for fragments. The sensitivity of MS and MS/MS analysis was estimated to be in the picomolar range or less. The peptide composition of the extracts was dependent on solvent formulation. Multiple peptides from apolipoproteins, complement proteins, coagulation factors, and many others were identified by X!Tandem with high mass accuracy of peptide ions and fragments from collision-induced dissociation. Many previously unreported posttranslational modifications of peptides including phosphorylations, oxidations, glycosylations, and others were detected with high mass accuracy and may be of clinical importance. About 4,630 redundant peptides were identified with 99% confidence separately, and together some 1,251 distinct proteins were identified with 99% confidence or greater using the Paragon algorithm.     

Controlling ghost traps in holographic optical tweezers

Computer-generated holograms displayed by phase-modulating spatial light modulators have become a well-established tool for beam shaping purposes in holographic optical tweezers. Still, the generation of light intensity patterns with high spatial symmetry and simultaneously without interfering ghost traps is a challenge. We have implemented an iterative Fourier transform algorithm that is capable of controlling these ghost traps and demonstrate the benefit of this approach in the experiment.     

Modifications of interface chemistry of LSM-YSZ composite by ceria nanoparticles

A porous composite electrode LSM-YSZ (lanthanum strontium manganite and yttria stabilized zirconia) was impregnated with different amounts of SDC (samarium substituted ceria) nanoparticles. The materials were investigated with X-ray diffraction, scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy to determine the microstructure, the interface chemistry and the surface chemistry of the various impregnated samples. The SDC nanoparticles cover the surface of the LSM-YSZ backbone to a large extent; they are approximately 5-20 nm in diameter and have a cubic crystal structure. Low concentrations of lanthanum and manganese originating from LSM were detected within SDC particles. It was also observed that the relative atomic concentration of strontium increased on the LSM-YSZ surface with increasing amount of SDC nanoparticles. These findings are related to the applied nanoparticle impregnation method. It is indicated that interactions between surfactant, nanoparticles, impregnation solution and the LSM-YSZ composite take place which can locally affect the surface and interface chemistry of the investigated materials.     

Analytical modelling of the in-plane compressive behaviour of pile fibres in Spacer Fabric Composites

The mechanical behaviour of Spacer Fabric Composites (SFC) is highly influenced by their pile fibres. Within this work, the in-plane compressive behaviour of pile fibres in SFC is investigated. An analytical model is developed based on rigid hinged struts. Therefore, a representative part of the continuous fibre is chosen. The bending behaviour and the interaction of the pile fibre with facesheet fibres is modelled using rotational and extension springs respectively. The system proposed is fully defined by three generalized coordinates. The total potential energy of the system is determined and used to obtain information about the deformation behaviour. (© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)     

Application of nonlinear continuum dislocation theory to antiplane shear

We apply the nonlinear dislocation theory to the problem of antiplane constrained shear in a single crystal with one slip system. By taking dissipation into account, the relaxed energy functional has to be minimized. We show that, up to a threshold strain, no dislocations are nucleated and therefore the plastic slip is zero. Since this threshold value depends on the width of the specimen, a size effect takes place. The stress strain curve turns out to be a hysteresis loop exhibiting the work hardening due to the dislocation pile-up. It is shown that the Bauschinger effect holds true. (© 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim)     

Forbidden Configurations and Product Constructions

A simple matrix is a (0,1)-matrix with no repeated columns. For a (0,1)-matrix F, we say that a (0,1)-matrix A has F as a configuration if there is a submatrix of A which is a row and column permutation of F (trace is the set system version of a configuration). Let \({\|A\|}\) denote the number of columns of A. We define \({{\rm forb}(m, F) = {\rm max}\{\|A\| \,:\, A}\) is m-rowed simple matrix and has no configuration F. We extend this to a family \({\mathcal{F} = \{F_1, F_2, \ldots , F_t\}}\) and define \({{\rm forb}(m, \mathcal{F}) = {\rm max}\{\|A\| \,:\, A}\) is m-rowed simple matrix and has no configuration \({F \in \mathcal{F}\}}\) . We consider products of matrices. Given an m ₁ × n ₁ matrix A and an m ₂ × n ₂ matrix B, we define the product A × B as the (m ₁ + m ₂) × n ₁ n ₂ matrix whose columns consist of all possible combinations obtained from placing a column of A on top of a column of B. Let I _k denote the k × k identity matrix, let \({I_k^{c}}\) denote the (0,1)-complement of I _k and let T _k denote the k × k upper triangular (0,1)-matrix with a 1 in position i, j if and only if i ≤ j. We show forb(m, {I ₂ × I ₂, T ₂ × T ₂}) is \({\Theta(m^{3/2})}\) while obtaining a linear bound when forbidding all 2-fold products of all 2 × 2 (0,1)-simple matrices. For two matrices F, P, where P is m-rowed, let \({f(F, P) = {\rm max}_{A} \{\|A\| \,:\,A}\) is m-rowed submatrix of P with no configuration F}. We establish f(I ₂ × I ₂, I _m/2 × I _m/2) is \({\Theta(m^{3/2})}\) whereas f(I ₂ × T ₂, I _m/2 × T _m/2) and f(T ₂ × T ₂, T _m/2 × T _m/2) are both \({\Theta(m)}\) . Additional results are obtained. One of the results requires extensive use of a computer program. We use the results on patterns due to Marcus and Tardos and generalizations due to Klazar and Marcus, Balogh, Bollobás and Morris.     

Travelling waves in dilatant non-Newtonian thin films

We prove the existence of a travelling wave solution for a gravity-driven thin film of a viscous and incompressible dilatant fluid coated with an insoluble surfactant. The governing system of second order partial differential equations for the film's height h and the surfactant's concentration γ are derived by means of lubrication theory applied to the non-Newtonian Navier–Stokes equations.     

Smart sampling as the “Spot-on” Method for LC-MS protein analysis from dried blood spots

This perspective explores the feasibility of smart sampling with dried blood spots for the determination of proteins and peptides from human biomatrices using liquid chromatography coupled to mass spectrometry for clinical purposes. The focus is on innovative approaches to transform filter paper from a mere sample carrier to an active element in sample preparation, with the aim of reducing the need for extensive and intensive sample preparation in the conventional sense. Specifically, we discuss the use of modified cellulose to integrate sample preparation at an early stage of sample handling. The use of paper immobilized with either trypsin or monoclonal antibodies for protein digestion and affinity clean-up is discussed as a potential benefit of starting sample preparation instantly at the moment of sampling to optimize time efficiency and enable faster analysis, diagnosis, and follow-up of patients.