Electrophoresis of mutant proteins in inherited diseases

The electrophoretic approaches for detection of mutant proteins in inherited diseases are briefly reviewed and discussed. Mutation of a protein, known to be associated with a specific inherited disease, is detected by immunoblotting, immunoprecipitation or enzyme staining, combined with various electrophoretic techniques. Some instrumental and technological devices for two-dimensional electrophoresis have been reported for the screening of mutant proteins in diseases of currently unknown etiology.     

The application of C-N.M.R. spectroscopy to the determination of the stereochemistry of Diels-Alder adducts

Long-range (³J) ¹³C-H coupling is a reliable probe to evaluate the stereochemistry of cycloaddition products. The method is best applied to carbonyl containing adducts but not restricted to them. Several structures have been revised and new ones evaluated.     

Vinylpolymerisation mit Metall-Komplexen. 27. Mitt. Mechanistische Untersuchung der Vinylpolymerisation mit Polyvinylamin-Cu-(II)-Chelaten in Dimethylsulfoxid-Lösung

Ohne Zusammenfassung     

Analysis of the reactivity of materials having two different kinds of functional groups using the A″-McKay plot method

The hydrogen-isotope exchange reaction between HTO vapor (as a gaseous material) andp-aminophenol (or -alanine) (as a solid material) having two different kinds of functional groups, has been studied to reveal the reactivity of the compounds. The reaction has been analyzed by the A-McKay plot method and the rate constants (k) for each group have been obtained. Comparison ofk leads to the following: (1) the effect of the NH₂ group inp-aminophenol on the reactivity is greater than that in -alanine, and (2) the reactivity of compounds having two different kinds of functional groups can be analyzed by the A-McKay plot method only.     

2-(8-quinolylazo)-7-phenylazochromotropic acid as an analytical reagent

2-(8-Quinolylazo)-7-phenylazochromotropic acid (QAPAC) has been synthesized by coupling diazotized 8-aminoquinoline with 2-phenylazochromotropic acid as well as by coupling diazotized aniline with 2-(8-quinolylazo)chromotropic acid. The properties of QAPAC and the spectrophotometric characteristics of some metal-QAPAC complexes are reported.     

Solid-liquid equilibrium diagrams of common ion binary salt hydrate mixtures involving nitrates and chlorides of magnesium, cobalt, nickel, manganese, and iron(III)

The solid-liquid equilibrium diagrams of binary mixtures involving magnesium nitrate hexahydrate with cobalt nitrate hexahydrate, nickel nitrate hexahydrate (partly), manganese nitrate tetrahydrate, and iron(III) nitrate nonahydrate and of magnesium chloride hexahydrate with cobalt and nickel chlorides hexahydrates and manganese chloride tetrahydrate, and the of two manganese salts were determined. Those diagrams that showed a simple eutectic were fitted by the Ott equation and where the required BET parameters were available, the magnesium salt rich parts of the liquidus were modeled by means of this method.