A variety of novel perfluorocycloalkene derivatives e.g. (1) – (5) may be obtained via various fluoride-ion induced reactions involving perfluorocycloalkenes. Also, pyridine-induced oligomerisation of perfluorocyclobutene gives (3), (4), and (6). Some of the chemistry of these and related compounds will be described and compared with chemistry of (7).相似文献
Electrochemical methods can be applied in a number of ways as detection systems for competitive immunoassays, especially through the use of redox-labelled antigens. To overcome the problem of detection of such conjugates at low concentration, a system is described in which a poly(vinylferrocene)-modified glassy carbon electrode is used to accumulate charge from solution glucose/glucose oxidase via the intermediacy of a redox-labelled antigen, cycled repeatedly between the enzyme and the electrode. The charge accumulated by the electrode is measured electrochemically by returning the electrode to its original potential and integrating the resulting current-time transient. The charge is proportional to the concentration of redox-labelled antigen in solution and to the charge-accumulation time. In this way 1,1'-dimethylferrocene-ethan-1-ol-2-amine was reproducibly detected to 0.9 nM, with a 10 min incubation period. The 8-ferrocenyl conjugate of theophylline was characterised as a model system for possible application in a higher sensitivity electrochemical immunoassay. 相似文献
Model compounds related to the important elastomer derived from CH22=CF2/CFCF3 are synthesised from telomers (CF3)2CF(CH2CF2nI, by coupling and by fluorodeiodination reactions. These models, in reactions with bases, give information relating to mechanism of cross-linking of the polymer and indications of factors that limit its working life. Novel use of SbF5 for dehydrofluorination in synthesis of fluorinated mono-enes and di-enes is described. 相似文献
Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules
to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid
by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). Here we describe
an automated microfluidic, microline-based assembly that uses LabView-controlled actuatable valves and a PCR machine, and
which is capable of the selection and synthesis of an anti-lysozyme aptamer as verified by sequence analysis. The microfluidic
prototype described is 1) a simple apparatus that is relatively inexpensive to assemble, making automated aptamer selection
accessible to many investigators, and 2) useful for the continued “morphing” of macro→meso→microfabricated structures until
a convergence to a few functional systems evolves and emerges, partly or completely achieving simpler, smaller and more rapid
SELEX applications. 相似文献